Determination of N-acetylcysteine in the presence of ciprofloxacin or   levofloxacin in microparticulate dry powder inhalers

Yağmur Akdağ ÇAYLI; Selma ŞAHİN; Emirhan NEMUTLU; Levent ÖNER

Makale detayları

Determination of N-acetylcysteine in the presence of ciprofloxacin or levofloxacin in microparticulate dry powder inhalers

Yazarlar:

Yağmur Akdağ ÇAYLI | Selma ŞAHİN | Emirhan NEMUTLU | Levent ÖNER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2019 Vol.43 Issue 3, pp.846-856

Belge Tipi:

Makale

Yayın Tarihi:

5/2019

Belge Dili:

Türkçe

Document ID (Org No.):

2000725731

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

A fast and easy method was validated for simultaneous determination of   ciprofloxacin hydrochloride monohydrate (CP), levofloxacin hemihydrate (LV),   and N-acetylcysteine (NAC) in samples. The analysis was performed on a   C1818_{18} column (250 x 4.6 mm, 5 μm) (Inertsil ODS-3V) using   an isocratic elution method with a mobile phase composed of 25 mM   KH22_{2}PO44_{4} (pH 3.0) and methanol (72:28, v/v) at a flow rate of 1   mL/min. UV detection was performed at 214 nm for NAC and 293 nm for CP and   LV. The method was validated for linearity, accuracy, precision   (repeatability and reproducibility), specificity, sensitivity, and   stability. The calibration study using several media demonstrated that the   calibration curves were linear for all compounds in all media (R22^{2}   > 0.9993). The limit of detection was 0.098 μg/mL for CP,   0.049 μg/mL for LV, and 0.487 μg/mL for NAC. The limit of   quantification was 0.328 μg/mL for CP, 0.165 μg/mL for LV, and   1.624 μg/mL for NAC. Precision and accuracy values of the method   fulfilled the required limits. All these outcomes demonstrate that the   validated HPLC method is appropriate for simultaneous analysis of CP, LV,   and NAC in samples for content uniformity of dry powder inhaler and   permeability studies.

Makale detayları

Determination of N-acetylcysteine in the presence of ciprofloxacin or levofloxacin in microparticulate dry powder inhalers

Yazarlar: Yağmur Akdağ ÇAYLI | Selma ŞAHİN | Emirhan NEMUTLU | Levent ÖNER

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2019 Vol.43 Issue 3, pp.846-856

Belge Tipi: Makale

Yayın Tarihi: 5/2019

Belge Dili: Türkçe

Document ID (Org No.): 2000725731

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

Yağmur Akdağ ÇAYLI | Selma ŞAHİN | Emirhan NEMUTLU | Levent ÖNER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2019 Vol.43 Issue 3, pp.846-856

Belge Tipi:

Makale

Yayın Tarihi:

5/2019

Belge Dili:

Türkçe

Document ID (Org No.):

2000725731

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527