Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection

Gizem ERENSOY; Duygu TAŞKIN; Gamze ÖZGÜL ARTUÇ; Elif ÖZDEMİR; Sumru ÖZKIRIMLI

Makale detayları

Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection

Yazarlar:

Gizem ERENSOY | Duygu TAŞKIN | Gamze ÖZGÜL ARTUÇ | Elif ÖZDEMİR | Sumru ÖZKIRIMLI

Yayıncı:

Istanbul University

Ana Başlıklar:

Farmakoloji ve terapötik | Sağlık teşvikleri

Dergi:

İstanbul Journal of Pharmacy 2020 Vol.50 Issue 2, pp.149-152

Belge Tipi:

Makale

Yayın Tarihi:

8/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000512973

Sıklık:

Aylık

eISSN:

2587-2087

Özet:

 

Background and Aims: An HPLC method with photodiode array detector on a chiral column was proposed for enantioselective determination of chlorpheniramine (CLP) enantiomers in dosage forms. Methods: The enantioselective determination was achieved on amylose tris(3,5-dimethylphenylcarbamate) column, using n-hexane-(propan-2-ol)-diethylamine (97.5:2.5:0.025, v/v/v) mobile phase. The peaks were detected at 258 nm. Diphenhydramine was used as an internal standard (IS). A new sample preparation procedure was developed to avoid the interference of the other ingredients present in the formulations. Results: Limit of quantification of the proposed method was 0.88 and 1.31 μg/mL for S-(+)-CLP and R-(-)-CLP, respectively. Conclusion: The method is linear, sensitive, specific and can be used for the enantioselective assay of CLP enantiomers in pharmaceutical formulations.

Makale detayları

Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection

Yazarlar: Gizem ERENSOY | Duygu TAŞKIN | Gamze ÖZGÜL ARTUÇ | Elif ÖZDEMİR | Sumru ÖZKIRIMLI

Yayıncı: Istanbul University

Ana Başlıklar: Farmakoloji ve terapötik | Sağlık teşvikleri

Dergi: İstanbul Journal of Pharmacy 2020 Vol.50 Issue 2, pp.149-152

Belge Tipi: Makale

Yayın Tarihi: 8/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000512973

Sıklık: Aylık

eISSN: 2587-2087

Sayı

Yazarlar:

Gizem ERENSOY | Duygu TAŞKIN | Gamze ÖZGÜL ARTUÇ | Elif ÖZDEMİR | Sumru ÖZKIRIMLI

Yayıncı:

Istanbul University

Ana Başlıklar:

Farmakoloji ve terapötik | Sağlık teşvikleri

Dergi:

İstanbul Journal of Pharmacy 2020 Vol.50 Issue 2, pp.149-152

Belge Tipi:

Makale

Yayın Tarihi:

8/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000512973

Sıklık:

Aylık

eISSN:

2587-2087