The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea

Yusuf Haydar ERTEKİN; Sedat ARIKAN; İhsan KARABOGA

doi:https://doi.org/10.22391/fppc.658034

Makale detayları

The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea

Yazarlar:

Yusuf Haydar ERTEKİN | Sedat ARIKAN | İhsan KARABOGA

Ana Başlıklar:

Hastalıklar

Dergi:

Family Practice and Palliative Care 2019 Vol.4 Issue 3, pp.126-129

Belge Tipi:

Makale

Yayın Tarihi:

12/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000429385

DOI:

https://doi.org/10.22391/fppc.658034

Özet:

 


Introduction: To investigate the effect
of ozone (O3) therapy on cornea subjected to systemically used high dose
methylprednisolone (MP) in a rabbit model. 


Methods:  Twenty-four New Zealand White adult male
rabbits were randomly divided into three equal groups as containing eight
animals. The first group (n = 8) was used as the control group and nothing was
applied to them, whereas the other 2 groups named as steroid groups were
subjected to IM methylprednisolone injection at a dose of 20 mg/kg/day for
three days. After three days of MP administration, only the third group was
treated with 50-µg/mL O3 (20 mL O3) through the rectal insufflation for 14
sessions. The histopathological examination of corneas of three groups were
made, and they were also assessed regarding the expression of hypoxia-inducible
factor-1 α (HIF-1α).


Results: It was determined that systemically
administered high dose MP caused erosion and necrosis in corneal epithelium and
stromal disintegrations in corneal stroma in steroid groups (Group 2 and Group
3). In the MP + O3 group (Group 3), the histopathological findings were mild.
The expression of HIF1-α in the cornea of Group1 (control group), Group 2 (MP),
and Group 3 (MP-O3) was measured as, 17.9±9.6%, 3.1±1.0% and 6.4±1.9% respectively.


Conclusions: MP and MP-O3 therapy decreased HIF-1a expression in rabbit cornea in
both intervention groups. Between these two groups, HIF-1α expression remained
relatively high in the MP-O3 group than in the MP group alone.


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Makale detayları

The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea

Yazarlar: Yusuf Haydar ERTEKİN | Sedat ARIKAN | İhsan KARABOGA

Ana Başlıklar: Hastalıklar

Dergi: Family Practice and Palliative Care 2019 Vol.4 Issue 3, pp.126-129

Belge Tipi: Makale

Yayın Tarihi: 12/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000429385

DOI: https://doi.org/10.22391/fppc.658034

Sayı

Yazarlar:

Yusuf Haydar ERTEKİN | Sedat ARIKAN | İhsan KARABOGA

Ana Başlıklar:

Hastalıklar

Dergi:

Family Practice and Palliative Care 2019 Vol.4 Issue 3, pp.126-129

Belge Tipi:

Makale

Yayın Tarihi:

12/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000429385

DOI:

https://doi.org/10.22391/fppc.658034