Cytogenetic effects of endogenous sex hormones depending on smoking habits

Handan ERBOĞA; Hasan Basri İLA

Makale detayları

Cytogenetic effects of endogenous sex hormones depending on smoking habits

Yazarlar:

Handan ERBOĞA | Hasan Basri İLA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 6, pp.709-715

Belge Tipi:

Makale

Yayın Tarihi:

5/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812334

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

This study was conducted with the aim of determining the effects of periodical hormonal fluctuations related to the menstrual cycle on chromosome sensitivity and cytotoxicity. The study group consisted of 8 healthy donors (4 nonsmokers and 4 smokers). Cytogenetic tests were done in vitro (in test tubes), and the known mutagenic effect of mitomycin C was added to determine chromosome sensitivity. Blood was drawn from the donors at specified time intervals (follicular phase, ovulation phase, and luteal phase), and control groups and mitomycin C-treated groups were formed. In the controls, the highest sister chromatid exchange frequency was detected in the follicular phase and the lowest frequency was detected in the luteal phase of nonsmokers. In smokers, the highest sister chromatid exchange frequency was detected in the ovulation phase and the lowest frequency was detected in the luteal phase. In terms of chromosomal aberrations, the highest values were detected in the follicular phase and the lowest values were detected in the luteal phase in nonsmokers. On the contrary, the highest rate of anomaly was detected in the luteal phase and the lowest rate of anomaly was detected in the follicular phase in smokers. However, there was no statistically significant difference between these findings. The data of the MMC application were similar in both groups. In this study, both the follicular phase and the ovulation phase showed slightly higher chromosome sensitivity, while the chromosomes in the luteal phase were the most stable. These results are probably due to hormonal fluctuation.

 

This study was conducted with the aim of determining the effects of periodical hormonal fluctuations related to the menstrual cycle on chromosome sensitivity and cytotoxicity. The study group consisted of 8 healthy donors (4 nonsmokers and 4 smokers). Cytogenetic tests were done in vitro (in test tubes), and the known mutagenic effect of mitomycin C was added to determine chromosome sensitivity. Blood was drawn from the donors at specified time intervals (follicular phase, ovulation phase, and luteal phase), and control groups and mitomycin C-treated groups were formed. In the controls, the highest sister chromatid exchange frequency was detected in the follicular phase and the lowest frequency was detected in the luteal phase of nonsmokers. In smokers, the highest sister chromatid exchange frequency was detected in the ovulation phase and the lowest frequency was detected in the luteal phase. In terms of chromosomal aberrations, the highest values were detected in the follicular phase and the lowest values were detected in the luteal phase in nonsmokers. On the contrary, the highest rate of anomaly was detected in the luteal phase and the lowest rate of anomaly was detected in the follicular phase in smokers. However, there was no statistically significant difference between these findings. The data of the MMC application were similar in both groups. In this study, both the follicular phase and the ovulation phase showed slightly higher chromosome sensitivity, while the chromosomes in the luteal phase were the most stable. These results are probably due to hormonal fluctuation.

Makale detayları

Cytogenetic effects of endogenous sex hormones depending on smoking habits

Yazarlar:

Handan ERBOĞA | Hasan Basri İLA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 6, pp.709-715

Belge Tipi:

Makale

Yayın Tarihi:

5/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812334

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Menstrual cycles

endogenous sex hormones

chromosome sensitivity

sister chromatid exchange

chromosome aberrations

Sayı

Makale detayları

Cytogenetic effects of endogenous sex hormones depending on smoking habits

Yazarlar: Handan ERBOĞA | Hasan Basri İLA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2013 Vol.37 Issue 6, pp.709-715

Belge Tipi: Makale

Yayın Tarihi: 5/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000812334

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Menstrual cycles

endogenous sex hormones

chromosome sensitivity

sister chromatid exchange

chromosome aberrations

Sayı

Yazarlar:

Handan ERBOĞA | Hasan Basri İLA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 6, pp.709-715

Belge Tipi:

Makale

Yayın Tarihi:

5/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812334

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152