Molecular Breeding of Selçuklu-97 Durum Wheat Cultivar for Some Genes  Affecting Pasta Quality

Özlem ATEŞ SÖNMEZOĞLU; Ahmet YILDIRIM; Tuğba ESERKAYA GÜLEÇ; Nejdet KANDEMİR; Abdulvahit SAYASLAN; Mehmet KOYUNCU

Makale detayları

Molecular Breeding of Selçuklu-97 Durum Wheat Cultivar for Some Genes Affecting Pasta Quality

Yazarlar:

Özlem ATEŞ SÖNMEZOĞLU | Ahmet YILDIRIM | Tuğba ESERKAYA GÜLEÇ | Nejdet KANDEMİR | Abdulvahit SAYASLAN | Mehmet KOYUNCU

Yayıncı:

Nobel Bilim ve Araştırma Merkezi Limited

Dergi:

Journal of Applied Biological Sciences 2010 Vol.4 Issue 2, pp.51-55

Belge Tipi:

Makale

Yayın Tarihi:

6/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000831621

Sıklık:

Üç Yıllık

eISSN:

2146-0108

Özet:

 

The most efficient way of producing and supplying proper raw material desired by the pasta industry is the development of high quality durum wheat varieties. Therefore, quality of Turkish durum wheat varieties should be improved using modern breeding methods without adversely affecting their yields.In this study, important genes (y-gliadin 45 and LMW-2 glutenin) affecting the quality of pasta products were transferred to a Turkish durum wheat variety, Selçuklu-97, in a backcross breeding method in combination with marker assisted selection (MAS). A Canadian durum wheat cultivar with high quality, Kyle, was used as the donor parent. Each of F1 and backcross (BC) plants was backcrossed four times to the recurrent parent and in all of the generations, backcrossed plants carrying the targeted QTLs were selected by the MAS. The MAS method was employed in combination with embryo culture and rapid plant growth in a controlled greenhouse conditions. In identifying and transferring processes of the gene regions, molecular DNA markers (SSR, STS and GAG) were employed with A-PAGE and SDS-PAGE methods. A-PAGE was used for selection of y-gliadin 45, SDS-PAGE for selection of LMW-2 glutenins, and four SSR primers (Xgwm550, Xgwm608, Stm553actc, Stm542acag), one STS primer and one PCR primer  linked to Gli-B1 and Glu-B3 loci for selection of y-gliadin 45 and LMW-2 glutenins either all together or alternatively. As a result, the study was completed in three years instead of six years required in a classical backcross breeding study, meaning about 50 % time saving, and obtained a high quality candidate variety.

Makale detayları

Molecular Breeding of Selçuklu-97 Durum Wheat Cultivar for Some Genes Affecting Pasta Quality

Yazarlar: Özlem ATEŞ SÖNMEZOĞLU | Ahmet YILDIRIM | Tuğba ESERKAYA GÜLEÇ | Nejdet KANDEMİR | Abdulvahit SAYASLAN | Mehmet KOYUNCU

Yayıncı: Nobel Bilim ve Araştırma Merkezi Limited

Dergi: Journal of Applied Biological Sciences 2010 Vol.4 Issue 2, pp.51-55

Belge Tipi: Makale

Yayın Tarihi: 6/2010

Belge Dili: Türkçe

Document ID (Org No.): 2000831621

Sıklık: Üç Yıllık

eISSN: 2146-0108

Sayı

Yazarlar:

Özlem ATEŞ SÖNMEZOĞLU | Ahmet YILDIRIM | Tuğba ESERKAYA GÜLEÇ | Nejdet KANDEMİR | Abdulvahit SAYASLAN | Mehmet KOYUNCU

Yayıncı:

Nobel Bilim ve Araştırma Merkezi Limited

Dergi:

Journal of Applied Biological Sciences 2010 Vol.4 Issue 2, pp.51-55

Belge Tipi:

Makale

Yayın Tarihi:

6/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000831621

Sıklık:

Üç Yıllık

eISSN:

2146-0108