Is leptin receptor expression triggered in the case of embryo transfer to endometrium coculture?

İskender KAPLANOĞLU; Gülnur Take KAPLANOĞLU; Özgür ÇINAR; Güleser GÖKTAŞ; Serdar DİLBAZ; Cemile Merve SEYMEN

Makale detayları

Is leptin receptor expression triggered in the case of embryo transfer to endometrium coculture?

Yazarlar:

İskender KAPLANOĞLU | Gülnur Take KAPLANOĞLU | Özgür ÇINAR | Güleser GÖKTAŞ | Serdar DİLBAZ | Cemile Merve SEYMEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2019 Vol.49 Issue 4, pp.1014-1018

Belge Tipi:

Makale

Yayın Tarihi:

7/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000749960

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Özet:

 

Background/aim: A  synchronized  dialogue  between  maternal  and  embryonic  tissues  is  required  for  successful  implantation.  Low  uterine  receptivity  is  responsible  for  two-thirds  of  implantation  failures  and  leptin  is  effective  in  the  physiology  of  reproduction  by  binding to specific receptors. In this study, we investigateleptin receptor expression in cases of embryo transfer to endometrial coculture. Materials and methods: Biopsy materials were taken from 20 females with indication for coculture application and were cultured in an appropriate medium after the epithelial cells were isolated. The grown cells were cultured in chamber slides as the first group. For the second group, day 3 embryo was added to chamber slides and the development was observed. The embryo was transferred 1 or 2 days later and other cells (after the transfer process) were used to form the second group. After fixation, immunohistochemical staining was performed with anti-leptin primary antibody. Results:  Regarding  the  coculture  without  embryo  transfer,  moderate  leptin  receptor  immunoreactivity  was  seen  in  the  perinuclear  region and the cell membrane. Also, regarding the coculture with embryo transfer, moderate leptin receptor immunoreactivity was seen in the cytoplasm and strong leptin receptor immunoreactivity was seen in the cell membrane.Conclusion: Embryo transfer to endometrium coculture triggers leptin receptor expression.

Makale detayları

Is leptin receptor expression triggered in the case of embryo transfer to endometrium coculture?

Yazarlar: İskender KAPLANOĞLU | Gülnur Take KAPLANOĞLU | Özgür ÇINAR | Güleser GÖKTAŞ | Serdar DİLBAZ | Cemile Merve SEYMEN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2019 Vol.49 Issue 4, pp.1014-1018

Belge Tipi: Makale

Yayın Tarihi: 7/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000749960

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Sayı

Yazarlar:

İskender KAPLANOĞLU | Gülnur Take KAPLANOĞLU | Özgür ÇINAR | Güleser GÖKTAŞ | Serdar DİLBAZ | Cemile Merve SEYMEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2019 Vol.49 Issue 4, pp.1014-1018

Belge Tipi:

Makale

Yayın Tarihi:

7/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000749960

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144