In Vitro Establishment Procedures of Dog Rose (Rosa canina)

Mehdi SHİRDEL; Ali Reza MOTALLEBİ-AZAR; Mansour MATLOOBİ; Sam MOKHTARZADEH; Fethi Ahmet ÖZDEMİR

Makale detayları

In Vitro Establishment Procedures of Dog Rose (Rosa canina)

Yazarlar:

Mehdi SHİRDEL | Ali Reza MOTALLEBİ-AZAR | Mansour MATLOOBİ | Sam MOKHTARZADEH | Fethi Ahmet ÖZDEMİR

Yayıncı:

Nobel Bilim ve Araştırma Merkezi Limited

Dergi:

Journal of Applied Biological Sciences 2017 Vol.11 Issue 2, pp.6-9

Belge Tipi:

Makale

Yayın Tarihi:

8/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000831087

Sıklık:

Üç Yıllık

eISSN:

2146-0108

Özet:

 


Dog rose (Rosa canina) is one of the medicinal plants with important role on vitamin C and active ingredients but a few researches has been conducted on the in vitro efficient establishment of this plant. Therefore, for optimization of establishment stage of R. canina, this study was carried out by 8 decontamination procedures included T1: 5% Sodium Hypochlorite, T2: 10% Povidone Iodine, T3: commercial Dettol, T4: commercial Banzalkonium Chloride, T5: Mercury (II) Chloride for 5 min, T6: Mercury (II) Chloride for 8 min, T7: 2.5% Sodium Hypochlorite with Cefotaxime and Tetracycline, T8: Mercury (II) Chloride for 2 min with Cefotaxime and Tetracycline. for elimination of bacteria and fungi contamination. In this study minimum browning percentage was observed in T7 and T8 (6 and 8% respectively), Bacterial contamination was completely controlled in T7 and T8, Fungi contamination percentage was completely controlled (100%) by T2, T3, T4, T6 and partially controlled by T8 and non-controlled by T7. Maximum bud break percentage was observed in T8, T7 and T2. The highest shoot length in T2 and T7 but the lowest shoot lengths were observed in T6. In T2, T3 and T7, node numbers were more than other decontamination treatments. T8 on the base of browning percentage, bacteria and fungi contamination percentage, bud break percentage was better than other procedures. T7 and T2 on the base of shoot length and node numbers were decontamination procedure better than other procedures. In this investigation decontamination procedures are considered as one the most important steps for successful implementation of biotechnological techniques for R. canina

.

Makale detayları

In Vitro Establishment Procedures of Dog Rose (Rosa canina)

Yazarlar: Mehdi SHİRDEL | Ali Reza MOTALLEBİ-AZAR | Mansour MATLOOBİ | Sam MOKHTARZADEH | Fethi Ahmet ÖZDEMİR

Yayıncı: Nobel Bilim ve Araştırma Merkezi Limited

Dergi: Journal of Applied Biological Sciences 2017 Vol.11 Issue 2, pp.6-9

Belge Tipi: Makale

Yayın Tarihi: 8/2017

Belge Dili: İngilizce

Document ID (Org No.): 2000831087

Sıklık: Üç Yıllık

eISSN: 2146-0108

Sayı

Yazarlar:

Mehdi SHİRDEL | Ali Reza MOTALLEBİ-AZAR | Mansour MATLOOBİ | Sam MOKHTARZADEH | Fethi Ahmet ÖZDEMİR

Yayıncı:

Nobel Bilim ve Araştırma Merkezi Limited

Dergi:

Journal of Applied Biological Sciences 2017 Vol.11 Issue 2, pp.6-9

Belge Tipi:

Makale

Yayın Tarihi:

8/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000831087

Sıklık:

Üç Yıllık

eISSN:

2146-0108