Role of KU70, SIRT1, and SIRT6 Proteins in Diabetic Rat Testis Tissue

Selim Demirtaş; Onur Ersoy; Gülnur Kızılay

Makale detayları

Role of KU70, SIRT1, and SIRT6 Proteins in Diabetic Rat Testis Tissue

Yazarlar:

Selim Demirtaş | Onur Ersoy | Gülnur Kızılay

Yayıncı:

Bingol University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi:

Türk Doğa ve Fen Dergisi Vol.13 Issue 3, pp.82-91

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328008

pISSN:

2149-6366

Anahtar kelimeler:

Özet:

 Diabetic male infertilitysubfertility is an important complication of diabetes. The molecular mechanisms responsible for this complication have not been thoroughly investigated. We aimed to elucidate the role of KU70, SIRT1, and SIRT6 proteins in diabetic testis. SpragueDawley male rats were maintained under stable laboratory conditions. The rats were divided into a control group n8 and a diabetes group n8 treated with a single dose of 50 mgkg streptozotocin. At the end of the 1-month experimental period, animals were sacrificed under anesthesia. Both testicles were removed, processed lightly, and studied through electron transmission microscopy and western blotting. Blood samples were collected for biochemical analysis. Histopathological analysis revealed that, in the diabetes group, the diaphragmatic tubule diameters and serum testosterone levels were decreased. KU70 immunoreactivity was statistically significantly increased, whereas SIRT1 and SIRT6 expression was significantly decreased compared with that observed in the control group. This is the first study to examine the expression of KU70, SIRT1, and SIRT6 in diabetic testicular tissue for the first time. According to the results, KU70, SIRT1, and SIRT6 may play an important role in cell apoptosis in diabetic testicular tissue. Importance of these proteins should be investigated further in additional quantitative studies.

 Diabetic male infertilitysubfertility is an important complication of diabetes. The molecular mechanisms responsible for this complication have not been thoroughly investigated. We aimed to elucidate the role of KU70, SIRT1, and SIRT6 proteins in diabetic testis. SpragueDawley male rats were maintained under stable laboratory conditions. The rats were divided into a control group n8 and a diabetes group n8 treated with a single dose of 50 mgkg streptozotocin. At the end of the 1-month experimental period, animals were sacrificed under anesthesia. Both testicles were removed, processed lightly, and studied through electron transmission microscopy and western blotting. Blood samples were collected for biochemical analysis. Histopathological analysis revealed that, in the diabetes group, the diaphragmatic tubule diameters and serum testosterone levels were decreased. KU70 immunoreactivity was statistically significantly increased, whereas SIRT1 and SIRT6 expression was significantly decreased compared with that observed in the control group. This is the first study to examine the expression of KU70, SIRT1, and SIRT6 in diabetic testicular tissue for the first time. According to the results, KU70, SIRT1, and SIRT6 may play an important role in cell apoptosis in diabetic testicular tissue. Importance of these proteins should be investigated further in additional quantitative studies.

 Diabetic male infertilitysubfertility is an important complication of diabetes. The molecular mechanisms responsible for this complication have not been thoroughly investigated. We aimed to elucidate the role of KU70, SIRT1, and SIRT6 proteins in diabetic testis. SpragueDawley male rats were maintained under stable laboratory conditions. The rats were divided into a control group n8 and a diabetes group n8 treated with a single dose of 50 mgkg streptozotocin. At the end of the 1-month experimental period, animals were sacrificed under anesthesia. Both testicles were removed, processed lightly, and studied through electron transmission microscopy and western blotting. Blood samples were collected for biochemical analysis. Histopathological analysis revealed that, in the diabetes group, the diaphragmatic tubule diameters and serum testosterone levels were decreased. KU70 immunoreactivity was statistically significantly increased, whereas SIRT1 and SIRT6 expression was significantly decreased compared with that observed in the control group. This is the first study to examine the expression of KU70, SIRT1, and SIRT6 in diabetic testicular tissue for the first time. According to the results, KU70, SIRT1, and SIRT6 may play an important role in cell apoptosis in diabetic testicular tissue. Importance of these proteins should be investigated further in additional quantitative studies.

 Diabetic male infertilitysubfertility is an important complication of diabetes. The molecular mechanisms responsible for this complication have not been thoroughly investigated. We aimed to elucidate the role of KU70, SIRT1, and SIRT6 proteins in diabetic testis. SpragueDawley male rats were maintained under stable laboratory conditions. The rats were divided into a control group n8 and a diabetes group n8 treated with a single dose of 50 mgkg streptozotocin. At the end of the 1-month experimental period, animals were sacrificed under anesthesia. Both testicles were removed, processed lightly, and studied through electron transmission microscopy and western blotting. Blood samples were collected for biochemical analysis. Histopathological analysis revealed that, in the diabetes group, the diaphragmatic tubule diameters and serum testosterone levels were decreased. KU70 immunoreactivity was statistically significantly increased, whereas SIRT1 and SIRT6 expression was significantly decreased compared with that observed in the control group. This is the first study to examine the expression of KU70, SIRT1, and SIRT6 in diabetic testicular tissue for the first time. According to the results, KU70, SIRT1, and SIRT6 may play an important role in cell apoptosis in diabetic testicular tissue. Importance of these proteins should be investigated further in additional quantitative studies.

Makale detayları

Role of KU70, SIRT1, and SIRT6 Proteins in Diabetic Rat Testis Tissue

Yazarlar:

Selim Demirtaş | Onur Ersoy | Gülnur Kızılay

Yayıncı:

Bingol University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi:

Türk Doğa ve Fen Dergisi Vol.13 Issue 3, pp.82-91

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328008

pISSN:

2149-6366

Bax/Bcl2-ratio

TUNEL assay

Rat

Apoptosis

Histology

Sayı

Makale detayları

Role of KU70, SIRT1, and SIRT6 Proteins in Diabetic Rat Testis Tissue

Yazarlar: Selim Demirtaş | Onur Ersoy | Gülnur Kızılay

Yayıncı: Bingol University

Ana Başlıklar: Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi: Türk Doğa ve Fen Dergisi Vol.13 Issue 3, pp.82-91

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001328008

pISSN: 2149-6366

Bax/Bcl2-ratio

TUNEL assay

Rat

Apoptosis

Histology

Sayı

Yazarlar:

Selim Demirtaş | Onur Ersoy | Gülnur Kızılay

Yayıncı:

Bingol University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi:

Türk Doğa ve Fen Dergisi Vol.13 Issue 3, pp.82-91

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328008

pISSN:

2149-6366