Evaluation of the Anti-Cancer Effect of TRAF2 and NCK In teracting Protein Kinase (TNIK) Inhibition in Breast Cancer Cells

Selin Zeynep Özçelik; Kaan Furkan Hamarat; Gamze Güney Eskiler; Süleyman Kaleli

Makale detayları

Evaluation of the Anti-Cancer Effect of TRAF2 and NCK In teracting Protein Kinase (TNIK) Inhibition in Breast Cancer Cells

Yazarlar:

Selin Zeynep Özçelik | Kaan Furkan Hamarat | Gamze Güney Eskiler | Süleyman Kaleli

Yayıncı:

Sakarya University

Ana Başlıklar:

Tıbbi Genetik (Kanser Genetiği hariç)

Dergi:

Sakarya Tıp Dergisi Vol.14 Issue 4, pp.398-406

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001334116

pISSN:

2146-409X

Anahtar kelimeler:

Özet:

 Objective: This study aimed to evaluate the anticancer effect of NCB-0846, a TNIK inhibitor, in MCF-7 cells and to assess its impact on the expression levels of NF-κB and TNFA at the gene level. Materials and Methods: The MCF-7 cell line was cultured at 37C in a 5 CO2 atmosphere using Dulbeccos Modified Eagle Medium DMEM supplemented with 10 fetal bovine serum FBS and antibiotics 50 IUmL penicillin and 50 mgmL streptomycin. Cell viability was analyzed using the CCK-8 assay to determine the cytotoxic effect of NCB- 0846. Acridine OrangePropidium Iodide AOPI staining was performed to evaluate the effect of NCB-0846 on cellular morphology in the MCF-7 cell line. Total RNA was isolated from cells treated with NCB-0846. Data analysis was performed using the SPSS 22.0 statistical program. Results: The data indicated that NCB-0846 significantly decreased the viability rates of MCF-7 cells in a dose-dependent manner 1-3 μM, p0.01. RT-PCR analysis revealed that the expression level of NFKB1 increased 5.4-fold compared to the control group in MCF-7 cells treated with NCB-0846 at the effective dose and duration p0.01. In contrast, the expression level of TNFA decreased to 0.4-fold compared to the control group p0.01. Conclusion: The results demonstrate that NCB-0846 induces changes in the mRNA levels of the NFKB1 and TNFA genes, which are associated with inflammatory signalling pathways in MCF-7 cells. However, further molecular analyses are necessary to clarify the effect of NCB-0846 on inflammation in breast cancer and other cancer types.

 Objective: This study aimed to evaluate the anticancer effect of NCB-0846, a TNIK inhibitor, in MCF-7 cells and to assess its impact on the expression levels of NF-κB and TNFA at the gene level. Materials and Methods: The MCF-7 cell line was cultured at 37C in a 5 CO2 atmosphere using Dulbeccos Modified Eagle Medium DMEM supplemented with 10 fetal bovine serum FBS and antibiotics 50 IUmL penicillin and 50 mgmL streptomycin. Cell viability was analyzed using the CCK-8 assay to determine the cytotoxic effect of NCB- 0846. Acridine OrangePropidium Iodide AOPI staining was performed to evaluate the effect of NCB-0846 on cellular morphology in the MCF-7 cell line. Total RNA was isolated from cells treated with NCB-0846. Data analysis was performed using the SPSS 22.0 statistical program. Results: The data indicated that NCB-0846 significantly decreased the viability rates of MCF-7 cells in a dose-dependent manner 1-3 μM, p0.01. RT-PCR analysis revealed that the expression level of NFKB1 increased 5.4-fold compared to the control group in MCF-7 cells treated with NCB-0846 at the effective dose and duration p0.01. In contrast, the expression level of TNFA decreased to 0.4-fold compared to the control group p0.01. Conclusion: The results demonstrate that NCB-0846 induces changes in the mRNA levels of the NFKB1 and TNFA genes, which are associated with inflammatory signalling pathways in MCF-7 cells. However, further molecular analyses are necessary to clarify the effect of NCB-0846 on inflammation in breast cancer and other cancer types.

 Objective: This study aimed to evaluate the anticancer effect of NCB-0846, a TNIK inhibitor, in MCF-7 cells and to assess its impact on the expression levels of NF-κB and TNFA at the gene level. Materials and Methods: The MCF-7 cell line was cultured at 37C in a 5 CO2 atmosphere using Dulbeccos Modified Eagle Medium DMEM supplemented with 10 fetal bovine serum FBS and antibiotics 50 IUmL penicillin and 50 mgmL streptomycin. Cell viability was analyzed using the CCK-8 assay to determine the cytotoxic effect of NCB- 0846. Acridine OrangePropidium Iodide AOPI staining was performed to evaluate the effect of NCB-0846 on cellular morphology in the MCF-7 cell line. Total RNA was isolated from cells treated with NCB-0846. Data analysis was performed using the SPSS 22.0 statistical program. Results: The data indicated that NCB-0846 significantly decreased the viability rates of MCF-7 cells in a dose-dependent manner 1-3 μM, p0.01. RT-PCR analysis revealed that the expression level of NFKB1 increased 5.4-fold compared to the control group in MCF-7 cells treated with NCB-0846 at the effective dose and duration p0.01. In contrast, the expression level of TNFA decreased to 0.4-fold compared to the control group p0.01. Conclusion: The results demonstrate that NCB-0846 induces changes in the mRNA levels of the NFKB1 and TNFA genes, which are associated with inflammatory signalling pathways in MCF-7 cells. However, further molecular analyses are necessary to clarify the effect of NCB-0846 on inflammation in breast cancer and other cancer types.

 Amaç: Bu çalışmanın amacı, TNIK inhibitörü olan NCB-0846nın MCF-7 hücrelerindeki antikanser etkisini değerlendirmek ve gen düzeyinde NF-κB ve TNFA ifade seviyeleri üzerindeki etkisini değerlendirmektir. Materyal ve Metot: MCF-7 hücre hattı, 10 fetal sığır serumu FBS ve antibiyotiklerle 50 IUmL penisilin ve 50 mgmL streptomisin desteklenmiş Dulbeccos Modified Eagle Medium DMEM kullanılarak 5 CO2 atmosferinde 37Cde kültüre edildi. Hücre canlılığı, NCB-0846nın sitotoksik etkisini belirlemek için CCK-8 testi kullanılarak analiz edildi. Akridin OranjPropidyum İyodür AOPI boyama, NCB-0846nın MCF-7 hücre hattındaki hücresel morfoloji üzerindeki etkisini değerlendirmek için gerçekleştirildi. Toplam RNA, NCB-0846 ile tedavi edilen hücrelerden izole edildi. Veri analizi SPSS 22.0 istatistik programı kullanılarak gerçekleştirildi. Bulgular: Veriler, NCB-0846nın MCF-7 hücrelerinin canlılık oranlarını doza bağlı bir şekilde önemli ölçüde azalttığını gösterdi 1-3 µM, p0,01. RT-PCR analizi, etkili doz ve sürede NCB-0846 ile tedavi edilen MCF-7 hücrelerinde NFKB1 ekspresyon düzeyinin kontrol grubuna kıyasla 5,4 kat arttığını ortaya koydu p0,01. Buna karşılık, TNFA ekspresyon düzeyi kontrol grubuna kıyasla 0,4 kat azaldı p0,01. Sonuç: Sonuçlar, NCB-0846nın MCF-7 hücrelerinde inflamatuar sinyal yollarıyla ilişkili olan NFKB1 ve TNFA genlerinin mRNA düzeylerinde değişikliklere neden olduğunu göstermektedir. Ancak, NCB-0846nın meme kanseri ve diğer kanser türlerindeki inflamasyon üzerindeki etkisini açıklığa kavuşturmak için daha fazla moleküler analiz gereklidir.

Makale detayları

Evaluation of the Anti-Cancer Effect of TRAF2 and NCK In teracting Protein Kinase (TNIK) Inhibition in Breast Cancer Cells

Yazarlar:

Selin Zeynep Özçelik | Kaan Furkan Hamarat | Gamze Güney Eskiler | Süleyman Kaleli

Yayıncı:

Sakarya University

Ana Başlıklar:

Tıbbi Genetik (Kanser Genetiği hariç)

Dergi:

Sakarya Tıp Dergisi Vol.14 Issue 4, pp.398-406

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001334116

pISSN:

2146-409X

Breast cancer

Inflammation

NCB-0846

TNIKMeme kanseri

Enflamasyon

NCB-0846

TNIK

Sayı

Makale detayları

Yazarlar: Selin Zeynep Özçelik | Kaan Furkan Hamarat | Gamze Güney Eskiler | Süleyman Kaleli

Yayıncı: Sakarya University

Ana Başlıklar: Tıbbi Genetik (Kanser Genetiği hariç)

Dergi: Sakarya Tıp Dergisi Vol.14 Issue 4, pp.398-406

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001334116

pISSN: 2146-409X

Sayı

Yazarlar:

Selin Zeynep Özçelik | Kaan Furkan Hamarat | Gamze Güney Eskiler | Süleyman Kaleli

Yayıncı:

Sakarya University

Ana Başlıklar:

Tıbbi Genetik (Kanser Genetiği hariç)

Dergi:

Sakarya Tıp Dergisi Vol.14 Issue 4, pp.398-406

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001334116

pISSN:

2146-409X