Determination of Antioxidant and Some Biochemical Properties of Different Extracts of Tribulus Terrestris

Müslüm Kuzu

Makale detayları

Determination of Antioxidant and Some Biochemical Properties of Different Extracts of Tribulus Terrestris

Yazarlar:

Müslüm Kuzu

Yayıncı:

Karabuk University

Ana Başlıklar:

Beslenme ve Diyetetik (Diğer)

Dergi:

Unika Sağlık Bilimleri Dergisi Vol.4 Issue 1, pp.694-708

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001315799

pISSN:

2757-6817

Anahtar kelimeler:

Özet:

 Objective: This study determined the antioxidant and total phenolic substance amounts of different extracts petroleum ether, chloroform, methanol, ethanol obtained from the iron Tribulus terrestris plant. Methods: Antioxidant activities of the extracts were examined using CUPRAC, FRAP, DPPH, and ABTS methods. In addition, its effects on acetylcholine esterase AChE and butyrylcholine esterase BChE activities were evaluated in vitro. Results: According to the findings, methanol extract had the highest total phenolic substance amount 63.594.33 μg gallic acidmg extract. In terms of CUPRAC reducing capacity, it was determined that methanol extract had the most substantial effect among the extracts, although it was lower than standard antioxidants. According to the FRAP method, methanol extract had the highest reduction capacity with a 1.47 μmol Fe2mg extract value. Similar results were seen with radical scavenging effects. It was the methanol extract with the highest radical scavenging capacity, according to DPPH and ABTS methods. It showed this effect with an IC50 value of 24.39 μgmL according to the DPPH method and an extract value of 0.47 μgμg according to the ABTS method. When the enzyme inhibition results were examined, it was seen that the methanol extract had the most potent inhibition effect with an IC50 value of 17.29 μgmL for the AChE enzyme and 18.23 μgmL for BChE. Conclusions: According to the results obtained, evidence was obtained that the extracts obtained from the iron thistle plant have an antioxidant effect and may be an option in the treatment of Alzheimers disease with their inhibition effect on AChE and BChE enzyme activities.

 Objective: This study determined the antioxidant and total phenolic substance amounts of different extracts petroleum ether, chloroform, methanol, ethanol obtained from the iron Tribulus terrestris plant. Methods: Antioxidant activities of the extracts were examined using CUPRAC, FRAP, DPPH, and ABTS methods. In addition, its effects on acetylcholine esterase AChE and butyrylcholine esterase BChE activities were evaluated in vitro. Results: According to the findings, methanol extract had the highest total phenolic substance amount 63.594.33 μg gallic acidmg extract. In terms of CUPRAC reducing capacity, it was determined that methanol extract had the most substantial effect among the extracts, although it was lower than standard antioxidants. According to the FRAP method, methanol extract had the highest reduction capacity with a 1.47 μmol Fe2mg extract value. Similar results were seen with radical scavenging effects. It was the methanol extract with the highest radical scavenging capacity, according to DPPH and ABTS methods. It showed this effect with an IC50 value of 24.39 μgmL according to the DPPH method and an extract value of 0.47 μgμg according to the ABTS method. When the enzyme inhibition results were examined, it was seen that the methanol extract had the most potent inhibition effect with an IC50 value of 17.29 μgmL for the AChE enzyme and 18.23 μgmL for BChE. Conclusions: According to the results obtained, evidence was obtained that the extracts obtained from the iron thistle plant have an antioxidant effect and may be an option in the treatment of Alzheimers disease with their inhibition effect on AChE and BChE enzyme activities.

 Objective: This study determined the antioxidant and total phenolic substance amounts of different extracts petroleum ether, chloroform, methanol, ethanol obtained from the iron Tribulus terrestris plant. Methods: Antioxidant activities of the extracts were examined using CUPRAC, FRAP, DPPH, and ABTS methods. In addition, its effects on acetylcholine esterase AChE and butyrylcholine esterase BChE activities were evaluated in vitro. Results: According to the findings, methanol extract had the highest total phenolic substance amount 63.594.33 μg gallic acidmg extract. In terms of CUPRAC reducing capacity, it was determined that methanol extract had the most substantial effect among the extracts, although it was lower than standard antioxidants. According to the FRAP method, methanol extract had the highest reduction capacity with a 1.47 μmol Fe2mg extract value. Similar results were seen with radical scavenging effects. It was the methanol extract with the highest radical scavenging capacity, according to DPPH and ABTS methods. It showed this effect with an IC50 value of 24.39 μgmL according to the DPPH method and an extract value of 0.47 μgμg according to the ABTS method. When the enzyme inhibition results were examined, it was seen that the methanol extract had the most potent inhibition effect with an IC50 value of 17.29 μgmL for the AChE enzyme and 18.23 μgmL for BChE. Conclusions: According to the results obtained, evidence was obtained that the extracts obtained from the iron thistle plant have an antioxidant effect and may be an option in the treatment of Alzheimers disease with their inhibition effect on AChE and BChE enzyme activities.

 Amaç: Bu çalışmada demir Tribulus terrestris demir diken bitkisinden elde edilen farklı ekstraktların petrol eteri, kloroform, metanol, etanol antioksidan ve toplam fenolik madde miktarını belirlendi. Gereç ve Yöntem: Ekstraktların antioksidan aktiviteleri CUPRAC, FRAP, DPPH ve ABTS yöntemleriyle incelendi. Bunun yanında asetilkolin esteraz AChE ve bütirilkolin esteraz BChE aktiviteleri üzerine etkileri in vitro olarak değerlendirildi. Bulgular: Elde edilen bulgulara göre toplam fenolik madde miktarı en fazla olan ekstraktın metanol ekstraktı olduğu görüldü 63,594,33 μg gallik asitmg ekstrakt. CUPRAC indirgeme kapasitesi bakımından standart antioksidanlara kıyasla düşük olmakla birlikte ekstraktlar arasında en güçlü etkiye metanol ekstraktının olduğu belirlendi. FRAP yöntemine göre yine metanol ekstraktı 1,47 μmol Fe2mg ekstrakt değeri ile en yüksek indirgeme kapasitesine sahipti. Benzer sonuçlar radikal giderme etkilerinde de görüldü. Hem DPPH hem de ABTS yöntemlerine göre radikal giderme kapasitesi en yüksek olan metanol ekstraktı idi. DPPH yöntemine göre 24,39 μgmL IC50 değeri ile, ABTS yöntemine göre ise 0,47 μg μg ekstrakt değerleri ile bu etkiyi gösterdi. Enzim inhibisyon sonuçlarına bakıldığında AChE enzimi için 17,29 μgmL, BChE için 18,23 μgmL IC50 değeri ile en güçlü inhibisyon etkisinin yine metanol ekstraktına sahip olduğu görüldü. Sonuç: Elde edilen sonuçlara göre demir diken bitkisinden elde edilen ekstraktların antioksidan etki gösterdiği, AChE ve BChE enzim aktiviteleri üzerine gösterdiği inhibisyon etkisi ile Alzheimer hastalığı tedavisinde bir seçenek olabileceği noktasında ön bilgiler elde edilmiş oldu.

Makale detayları

Determination of Antioxidant and Some Biochemical Properties of Different Extracts of Tribulus Terrestris

Yazarlar:

Müslüm Kuzu

Yayıncı:

Karabuk University

Ana Başlıklar:

Beslenme ve Diyetetik (Diğer)

Dergi:

Unika Sağlık Bilimleri Dergisi Vol.4 Issue 1, pp.694-708

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001315799

pISSN:

2757-6817

Antioxidant

Acetylcholine esterase

Butyrylcholine esterase

Tribulus terrestrisAntioksidan

Asetilkolin esteraz

Bütirilkolin esteraz

Tribulus terrestris

Sayı

Makale detayları

Yazarlar: Müslüm Kuzu

Yayıncı: Karabuk University

Ana Başlıklar: Beslenme ve Diyetetik (Diğer)

Dergi: Unika Sağlık Bilimleri Dergisi Vol.4 Issue 1, pp.694-708

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001315799

pISSN: 2757-6817

Sayı

Yazarlar:

Müslüm Kuzu

Yayıncı:

Karabuk University

Ana Başlıklar:

Beslenme ve Diyetetik (Diğer)

Dergi:

Unika Sağlık Bilimleri Dergisi Vol.4 Issue 1, pp.694-708

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001315799

pISSN:

2757-6817