Preliminary in vitro assessment of cytotoxic and genotoxic effects of avocado (Persea Americano) oil in breast cancer cell line (MCF-7)

Ayça Aktaş Şüküroğlu

Makale detayları

Preliminary in vitro assessment of cytotoxic and genotoxic effects of avocado (Persea Americano) oil in breast cancer cell line (MCF-7)

Yazarlar:

Ayça Aktaş Şüküroğlu

Yayıncı:

Adıyaman University

Ana Başlıklar:

Onkoloji ve Karsinogenez (Diğer)

Dergi:

Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.9 Issue 3, pp.162-168

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001305472

pISSN:

2458-9179

Anahtar kelimeler:

Özet:

 Aim: Toxicological evaluation is required to understand the safety of avocado Persea Americana oil for use as a food supplement. In this study, cytotoxic and genotoxic effects of avocado oil in MCF-7 cell line were evaluated. Materials and Methods: In this study, the MCF-7 was exposed to avocado oil 1, 10, 25 and 100 ppm for 24, 48 and 72 hrs to assess the cytotoxic and genotoxic effects. Results: IC50 of avocado oil were found to be 68.1, 62.8 and 64.3 ppm for 24, 48 and 72 hrs, respectively. There was a statistically significant decrease in cell polferation between the control and exposed groups p0.05. Micronucleus frequency was significantly increased compared with negative control p0.005. Conclusion: Results of the study, avocado oil had cytotoxic and genotoxic effects in a time and concentration dependent manner. Regular use of avocado oil as a dietary supplement has been shown to have a protective effect.

 Aim: Toxicological evaluation is required to understand the safety of avocado Persea Americana oil for use as a food supplement. In this study, cytotoxic and genotoxic effects of avocado oil in MCF-7 cell line were evaluated. Materials and Methods: In this study, the MCF-7 was exposed to avocado oil 1, 10, 25 and 100 ppm for 24, 48 and 72 hrs to assess the cytotoxic and genotoxic effects. Results: IC50 of avocado oil were found to be 68.1, 62.8 and 64.3 ppm for 24, 48 and 72 hrs, respectively. There was a statistically significant decrease in cell polferation between the control and exposed groups p0.05. Micronucleus frequency was significantly increased compared with negative control p0.005. Conclusion: Results of the study, avocado oil had cytotoxic and genotoxic effects in a time and concentration dependent manner. Regular use of avocado oil as a dietary supplement has been shown to have a protective effect.

 Aim: Toxicological evaluation is required to understand the safety of avocado Persea Americana oil for use as a food supplement. In this study, cytotoxic and genotoxic effects of avocado oil in MCF-7 cell line were evaluated. Materials and Methods: In this study, the MCF-7 was exposed to avocado oil 1, 10, 25 and 100 ppm for 24, 48 and 72 hrs to assess the cytotoxic and genotoxic effects. Results: IC50 of avocado oil were found to be 68.1, 62.8 and 64.3 ppm for 24, 48 and 72 hrs, respectively. There was a statistically significant decrease in cell polferation between the control and exposed groups p0.05. Micronucleus frequency was significantly increased compared with negative control p0.005. Conclusion: Results of the study, avocado oil had cytotoxic and genotoxic effects in a time and concentration dependent manner. Regular use of avocado oil as a dietary supplement has been shown to have a protective effect.

 Amaç: Avokado Persea Americana yağının gıda takviyesi olarak kullanımında güvenliğinin anlaşılması için toksikolojik değerlendirilme yapılması gerekmektedir. Planlanan bu çalışmada avokado yağının MCF-7 hücre hattındaki sitotoksik ve genotoksik etkileri değerlendirilmiştir. Gereç ve Yöntem: Bu çalışmada MCF-7 hücre hattı, avokado yağına 1, 10, 25 ve 100 ppm ile 24, 48 ve 72 maruz bırakılarak sitotoksik ve genotoksik etkisi değerlendirilmiştir. Bulgular: Avokado yağının IC50 değerleri 24, 48 ve 72 saat için sırasıyla; 68.1, 62.8 ve 64.3 ppm olarak bulunmuştur. Avokado yağının bütün maruziyet sürelerinde kontrol grubu ile maruziyet grupları arasında hücre poliferasyonundaki azalma istatiksel olarak anlamlı bulunmuştur p0,05. Avokado yağına maruziyetine bağlı mikroçekirdek frekansında, tüm dozlarda negatif kontrole göre önemli artış görülmüştür p0,005. Sonuç: Çalışmanın sonucunda avokado yağının MCF-7 hücre hattında zamana ve konstrasyona bağımlı olarak sitotoksik ve genotoksik etkilerinin olduğu görülmüştür. Avokado yağının gıda takviyesi olarak düzenli kullanılması sonucunda koruyucu etkisinin olabileceği görülmüştür.

Makale detayları

Preliminary in vitro assessment of cytotoxic and genotoxic effects of avocado (Persea Americano) oil in breast cancer cell line (MCF-7)

Yazarlar:

Ayça Aktaş Şüküroğlu

Yayıncı:

Adıyaman University

Ana Başlıklar:

Onkoloji ve Karsinogenez (Diğer)

Dergi:

Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.9 Issue 3, pp.162-168

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001305472

pISSN:

2458-9179

Avocado oil

Cytotoxicity

Genotoxicity

MCF-7

Cytokinesis-block micronucleus assayAvokado yağı

Sitotoksisite

Genotoksisite

MCF-7

Sitokinezin durdurulduğu mikroçekirdek yöntemi

Sayı

Makale detayları

Yazarlar: Ayça Aktaş Şüküroğlu

Yayıncı: Adıyaman University

Ana Başlıklar: Onkoloji ve Karsinogenez (Diğer)

Dergi: Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.9 Issue 3, pp.162-168

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001305472

pISSN: 2458-9179

Sayı

Yazarlar:

Ayça Aktaş Şüküroğlu

Yayıncı:

Adıyaman University

Ana Başlıklar:

Onkoloji ve Karsinogenez (Diğer)

Dergi:

Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.9 Issue 3, pp.162-168

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001305472

pISSN:

2458-9179