PCR detection of Brucella abortus in cow milk samples collected from Erzurum, Turkey

Tülin ARASOĞLU; Medine GÜLLÜCE; Hakan ÖZKAN; Ahmet ADIGÜZEL; Fikrettin ŞAHİN

Makale detayları

PCR detection of Brucella abortus in cow milk samples collected from Erzurum, Turkey

Yazarlar:

Tülin ARASOĞLU | Medine GÜLLÜCE | Hakan ÖZKAN | Ahmet ADIGÜZEL | Fikrettin ŞAHİN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 4, pp.501-508

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000753628

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

In this study, we designed and used 2 different type-specific polymerase chain reaction (PCR) methods for the detection of Brucella abortus.   Materials and methods: There were 2 different primer sets (B4/B5 and AF/AR) selected and used for the amplification of 2 different genes that are present in all biovars of Brucella species, including bcsp31, encoding 31-kDa immunogenic cell surface proteins, and omp25, which is known to be one of the virulence factors encoding outer membrane proteins of 26–23 kDa.    Results: The results showed that 273 (81.7%) and 317 (94.9%) out of 334 milk samples were positive for brucellosis, as detected by either both or one of the primer sets used in the present study, respectively. The detection limit of PCR assays for Brucella in milk samples was determined as 5 pg DNA for both of the primer sets.    Conclusion: These results suggest that the use of the specific PCR assay with the primer sets used in the study is a rapid, reliable, and accurate technique in comparison to traditional and conventional methods for the detection and diagnosis of Brucella spp. in milk samples.  Kew words: Brucella abortus, bovine milk, bcsp31-PCR, omp25-PCR.

 

In this study, we designed and used 2 different type-specific polymerase chain reaction (PCR) methods for the detection of Brucella abortus.   Materials and methods: There were 2 different primer sets (B4/B5 and AF/AR) selected and used for the amplification of 2 different genes that are present in all biovars of Brucella species, including bcsp31, encoding 31-kDa immunogenic cell surface proteins, and omp25, which is known to be one of the virulence factors encoding outer membrane proteins of 26–23 kDa.    Results: The results showed that 273 (81.7%) and 317 (94.9%) out of 334 milk samples were positive for brucellosis, as detected by either both or one of the primer sets used in the present study, respectively. The detection limit of PCR assays for Brucella in milk samples was determined as 5 pg DNA for both of the primer sets.    Conclusion: These results suggest that the use of the specific PCR assay with the primer sets used in the study is a rapid, reliable, and accurate technique in comparison to traditional and conventional methods for the detection and diagnosis of Brucella spp. in milk samples.  Kew words: Brucella abortus, bovine milk, bcsp31-PCR, omp25-PCR.

Makale detayları

PCR detection of Brucella abortus in cow milk samples collected from Erzurum, Turkey

Yazarlar:

Tülin ARASOĞLU | Medine GÜLLÜCE | Hakan ÖZKAN | Ahmet ADIGÜZEL | Fikrettin ŞAHİN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 4, pp.501-508

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000753628

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Turk J Med Sci 2013; 43(4): 501-508. Full text: pdf Other articles published in the same issue: Turk J Med Sci

vol.43

iss.4.

Sayı

Makale detayları

PCR detection of Brucella abortus in cow milk samples collected from Erzurum, Turkey

Yazarlar: Tülin ARASOĞLU | Medine GÜLLÜCE | Hakan ÖZKAN | Ahmet ADIGÜZEL | Fikrettin ŞAHİN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2013 Vol.43 Issue 4, pp.501-508

Belge Tipi: Makale

Yayın Tarihi: 3/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000753628

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Turk J Med Sci 2013; 43(4): 501-508. Full text: pdf Other articles published in the same issue: Turk J Med Sci

vol.43

iss.4.

Sayı

Yazarlar:

Tülin ARASOĞLU | Medine GÜLLÜCE | Hakan ÖZKAN | Ahmet ADIGÜZEL | Fikrettin ŞAHİN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 4, pp.501-508

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000753628

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144