Overexpression of the tomato pathogenesis-related gene SlPR-1.9 confers increased tolerance to salt stress in Arabidopsis thaliana

Kubra Yildiz; M. Aydin Akbudak

Makale detayları

Overexpression of the tomato pathogenesis-related gene SlPR-1.9 confers increased tolerance to salt stress in Arabidopsis thaliana

Yazarlar:

Kubra Yildiz | M. Aydin Akbudak

Yayıncı:

Akdeniz University

Ana Başlıklar:

Plant Biotechnology in Agriculture

Dergi:

Mediterranean Agricultural Sciences Vol.37 Issue 3, pp.147-154

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001332738

pISSN:

2528-9675

Anahtar kelimeler:

Özet:

 Pathogenesis-related PR proteins are essential components of plant defense mechanisms, responding to both biotic and abiotic stresses. Among these, PR-1 proteins feature a CAP Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 domain, which is crucial for immune responses and pathogen defense due to its ability to stabilize protein structures and interact with various molecules. This study investigated the role of the tomato PR-1 gene SlPR-1.9 in enhancing salt tolerance in Arabidopsis thaliana. The genes coding sequence was cloned and transferred into Arabidopsis to create SlPR-1.9 overexpression lines. These transgenic lines, alongside wild-type plants, were exposed to salt stress 150 mM NaCl to assess their tolerance. Morphological analysis revealed that the transgenic lines demonstrated greater resilience to salt stress compared to wild-type plants, with less severe leaf curling and color changes. Additionally, lower proline accumulation, a stress marker, in the transgenic lines indicated an enhanced adaptive response. Bioinformatics analysis of the protein encoded by SlPR-1.9, A0A3Q7HSC4, suggested a strong interaction with galactolipase. Expression analysis showed that SlPR-1.9 was mainly expressed in roots and during early fruit development, suggesting a significant role in root physiology and stress response. These findings indicate that overexpression of SlPR-1.9 can improve plant tolerance to salt stress, offering potential applications for enhancing crop resilience to environmental challenges.

 Pathogenesis-related PR proteins are essential components of plant defense mechanisms, responding to both biotic and abiotic stresses. Among these, PR-1 proteins feature a CAP Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 domain, which is crucial for immune responses and pathogen defense due to its ability to stabilize protein structures and interact with various molecules. This study investigated the role of the tomato PR-1 gene SlPR-1.9 in enhancing salt tolerance in Arabidopsis thaliana. The genes coding sequence was cloned and transferred into Arabidopsis to create SlPR-1.9 overexpression lines. These transgenic lines, alongside wild-type plants, were exposed to salt stress 150 mM NaCl to assess their tolerance. Morphological analysis revealed that the transgenic lines demonstrated greater resilience to salt stress compared to wild-type plants, with less severe leaf curling and color changes. Additionally, lower proline accumulation, a stress marker, in the transgenic lines indicated an enhanced adaptive response. Bioinformatics analysis of the protein encoded by SlPR-1.9, A0A3Q7HSC4, suggested a strong interaction with galactolipase. Expression analysis showed that SlPR-1.9 was mainly expressed in roots and during early fruit development, suggesting a significant role in root physiology and stress response. These findings indicate that overexpression of SlPR-1.9 can improve plant tolerance to salt stress, offering potential applications for enhancing crop resilience to environmental challenges.

 Pathogenesis-related PR proteins are essential components of plant defense mechanisms, responding to both biotic and abiotic stresses. Among these, PR-1 proteins feature a CAP Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 domain, which is crucial for immune responses and pathogen defense due to its ability to stabilize protein structures and interact with various molecules. This study investigated the role of the tomato PR-1 gene SlPR-1.9 in enhancing salt tolerance in Arabidopsis thaliana. The genes coding sequence was cloned and transferred into Arabidopsis to create SlPR-1.9 overexpression lines. These transgenic lines, alongside wild-type plants, were exposed to salt stress 150 mM NaCl to assess their tolerance. Morphological analysis revealed that the transgenic lines demonstrated greater resilience to salt stress compared to wild-type plants, with less severe leaf curling and color changes. Additionally, lower proline accumulation, a stress marker, in the transgenic lines indicated an enhanced adaptive response. Bioinformatics analysis of the protein encoded by SlPR-1.9, A0A3Q7HSC4, suggested a strong interaction with galactolipase. Expression analysis showed that SlPR-1.9 was mainly expressed in roots and during early fruit development, suggesting a significant role in root physiology and stress response. These findings indicate that overexpression of SlPR-1.9 can improve plant tolerance to salt stress, offering potential applications for enhancing crop resilience to environmental challenges.

 Pathogenesis-related PR proteins are essential components of plant defense mechanisms, responding to both biotic and abiotic stresses. Among these, PR-1 proteins feature a CAP Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 domain, which is crucial for immune responses and pathogen defense due to its ability to stabilize protein structures and interact with various molecules. This study investigated the role of the tomato PR-1 gene SlPR-1.9 in enhancing salt tolerance in Arabidopsis thaliana. The genes coding sequence was cloned and transferred into Arabidopsis to create SlPR-1.9 overexpression lines. These transgenic lines, alongside wild-type plants, were exposed to salt stress 150 mM NaCl to assess their tolerance. Morphological analysis revealed that the transgenic lines demonstrated greater resilience to salt stress compared to wild-type plants, with less severe leaf curling and color changes. Additionally, lower proline accumulation, a stress marker, in the transgenic lines indicated an enhanced adaptive response. Bioinformatics analysis of the protein encoded by SlPR-1.9, A0A3Q7HSC4, suggested a strong interaction with galactolipase. Expression analysis showed that SlPR-1.9 was mainly expressed in roots and during early fruit development, suggesting a significant role in root physiology and stress response. These findings indicate that overexpression of SlPR-1.9 can improve plant tolerance to salt stress, offering potential applications for enhancing crop resilience to environmental challenges.

Makale detayları

Overexpression of the tomato pathogenesis-related gene SlPR-1.9 confers increased tolerance to salt stress in Arabidopsis thaliana

Yazarlar:

Kubra Yildiz | M. Aydin Akbudak

Yayıncı:

Akdeniz University

Ana Başlıklar:

Plant Biotechnology in Agriculture

Dergi:

Mediterranean Agricultural Sciences Vol.37 Issue 3, pp.147-154

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001332738

pISSN:

2528-9675

SlPR-1.9

Salt stress

Arabidopsis thaliana

Pathogenesis-related proteins

GalactolipaseSlPR-1.9

Salt stress

Arabidopsis thaliana

Pathogenesis-related proteins

Galactolipase

Sayı

Makale detayları

Yazarlar: Kubra Yildiz | M. Aydin Akbudak

Yayıncı: Akdeniz University

Ana Başlıklar: Plant Biotechnology in Agriculture

Dergi: Mediterranean Agricultural Sciences Vol.37 Issue 3, pp.147-154

Belge Tipi: Makale

Yayın Tarihi: 6/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001332738

pISSN: 2528-9675

Sayı

Yazarlar:

Kubra Yildiz | M. Aydin Akbudak

Yayıncı:

Akdeniz University

Ana Başlıklar:

Plant Biotechnology in Agriculture

Dergi:

Mediterranean Agricultural Sciences Vol.37 Issue 3, pp.147-154

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001332738

pISSN:

2528-9675