Preparation of PLGA-PEG/Hydroxyapatite Composites via Simple Methodology of Film Formation and Assessment of Their Structural, Thermal, and Biological Features

Fatih Çiftçi; Ali Can Özarslan

Makale detayları

Preparation of PLGA-PEG/Hydroxyapatite Composites via Simple Methodology of Film Formation and Assessment of Their Structural, Thermal, and Biological Features

Yazarlar:

Fatih Çiftçi | Ali Can Özarslan

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Polymer Science and Technologies

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.10 Issue 4, pp.1123-1132

Belge Tipi:

Makale

Yayın Tarihi:

11/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294242

pISSN:

2149-0120

Anahtar kelimeler:

Özet:

 This study aimed to develop polymeric composite films suitable for applications in the field of bone tissue engineering. The preparation of PLGA-PEGHAP composite films was achieved using a simple methodology, including mixing, sonication, and casting-drying stages. Characterization analyses, including FTIR, SEM, TGA-DSC, and XRD, were conducted to assess the properties of the composite films. The results showed that the PEG polymer decreased the glass transition temperature of the composite, while the HAP did not change. Further, weight remaining  values of HAP, PLGA-PEG, and PLGA-PEGHAP were found as 94.04, 88.28, and 90.57, respectively. Thus, it can be concluded that HAP improves the thermal stability of PLGA-PEG. The outcomes of the analysis, encompassing the evaluation of physical, morphological, and thermal properties, demonstrate that the composite structure comprising PLGA and PEG polymers along with HAP ceramic material may attain the intended quality. Moreover, fluorescence microscopy was employed to visualize the interaction between cells and the composite films following DAPI staining to evaluate cell adhesion and proliferation on the PLGA-PEGHAP composite films. PLGA-PEGHAP composite films have no adverse effects on cells, such as toxicity, and they have also exhibited a favorable influence on cell proliferation, supporting an augmentation in cellular growth and adhesion. Overall, the results indicate that the synthesized PLGA-PEGHAP composite films may hold the potential to serve as a promising candidate for applications in the field of bone tissue engineering.

 This study aimed to develop polymeric composite films suitable for applications in the field of bone tissue engineering. The preparation of PLGA-PEGHAP composite films was achieved using a simple methodology, including mixing, sonication, and casting-drying stages. Characterization analyses, including FTIR, SEM, TGA-DSC, and XRD, were conducted to assess the properties of the composite films. The results showed that the PEG polymer decreased the glass transition temperature of the composite, while the HAP did not change. Further, weight remaining  values of HAP, PLGA-PEG, and PLGA-PEGHAP were found as 94.04, 88.28, and 90.57, respectively. Thus, it can be concluded that HAP improves the thermal stability of PLGA-PEG. The outcomes of the analysis, encompassing the evaluation of physical, morphological, and thermal properties, demonstrate that the composite structure comprising PLGA and PEG polymers along with HAP ceramic material may attain the intended quality. Moreover, fluorescence microscopy was employed to visualize the interaction between cells and the composite films following DAPI staining to evaluate cell adhesion and proliferation on the PLGA-PEGHAP composite films. PLGA-PEGHAP composite films have no adverse effects on cells, such as toxicity, and they have also exhibited a favorable influence on cell proliferation, supporting an augmentation in cellular growth and adhesion. Overall, the results indicate that the synthesized PLGA-PEGHAP composite films may hold the potential to serve as a promising candidate for applications in the field of bone tissue engineering.

 This study aimed to develop polymeric composite films suitable for applications in the field of bone tissue engineering. The preparation of PLGA-PEGHAP composite films was achieved using a simple methodology, including mixing, sonication, and casting-drying stages. Characterization analyses, including FTIR, SEM, TGA-DSC, and XRD, were conducted to assess the properties of the composite films. The results showed that the PEG polymer decreased the glass transition temperature of the composite, while the HAP did not change. Further, weight remaining  values of HAP, PLGA-PEG, and PLGA-PEGHAP were found as 94.04, 88.28, and 90.57, respectively. Thus, it can be concluded that HAP improves the thermal stability of PLGA-PEG. The outcomes of the analysis, encompassing the evaluation of physical, morphological, and thermal properties, demonstrate that the composite structure comprising PLGA and PEG polymers along with HAP ceramic material may attain the intended quality. Moreover, fluorescence microscopy was employed to visualize the interaction between cells and the composite films following DAPI staining to evaluate cell adhesion and proliferation on the PLGA-PEGHAP composite films. PLGA-PEGHAP composite films have no adverse effects on cells, such as toxicity, and they have also exhibited a favorable influence on cell proliferation, supporting an augmentation in cellular growth and adhesion. Overall, the results indicate that the synthesized PLGA-PEGHAP composite films may hold the potential to serve as a promising candidate for applications in the field of bone tissue engineering.

 This study aimed to develop polymeric composite films suitable for applications in the field of bone tissue engineering. The preparation of PLGA-PEGHAP composite films was achieved using a simple methodology, including mixing, sonication, and casting-drying stages. Characterization analyses, including FTIR, SEM, TGA-DSC, and XRD, were conducted to assess the properties of the composite films. The results showed that the PEG polymer decreased the glass transition temperature of the composite, while the HAP did not change. Further, weight remaining  values of HAP, PLGA-PEG, and PLGA-PEGHAP were found as 94.04, 88.28, and 90.57, respectively. Thus, it can be concluded that HAP improves the thermal stability of PLGA-PEG. The outcomes of the analysis, encompassing the evaluation of physical, morphological, and thermal properties, demonstrate that the composite structure comprising PLGA and PEG polymers along with HAP ceramic material may attain the intended quality. Moreover, fluorescence microscopy was employed to visualize the interaction between cells and the composite films following DAPI staining to evaluate cell adhesion and proliferation on the PLGA-PEGHAP composite films. PLGA-PEGHAP composite films have no adverse effects on cells, such as toxicity, and they have also exhibited a favorable influence on cell proliferation, supporting an augmentation in cellular growth and adhesion. Overall, the results indicate that the synthesized PLGA-PEGHAP composite films may hold the potential to serve as a promising candidate for applications in the field of bone tissue engineering.

Makale detayları

Preparation of PLGA-PEG/Hydroxyapatite Composites via Simple Methodology of Film Formation and Assessment of Their Structural, Thermal, and Biological Features

Yazarlar:

Fatih Çiftçi | Ali Can Özarslan

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Polymer Science and Technologies

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.10 Issue 4, pp.1123-1132

Belge Tipi:

Makale

Yayın Tarihi:

11/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294242

pISSN:

2149-0120

Bone tissue engineering

PLGA

PEG

Hydroxyapatite

Composite

Film

Sayı

Makale detayları

Preparation of PLGA-PEG/Hydroxyapatite Composites via Simple Methodology of Film Formation and Assessment of Their Structural, Thermal, and Biological Features

Yazarlar: Fatih Çiftçi | Ali Can Özarslan

Yayıncı: Turkish Chemical Society

Ana Başlıklar: Polymer Science and Technologies

Dergi: Journal of the Turkish Chemical Society Section A: Chemistry Vol.10 Issue 4, pp.1123-1132

Belge Tipi: Makale

Yayın Tarihi: 11/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001294242

pISSN: 2149-0120

Bone tissue engineering

PLGA

PEG

Hydroxyapatite

Composite

Film

Sayı

Yazarlar:

Fatih Çiftçi | Ali Can Özarslan

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Polymer Science and Technologies

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.10 Issue 4, pp.1123-1132

Belge Tipi:

Makale

Yayın Tarihi:

11/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294242

pISSN:

2149-0120