INVESTIGATION OF THE EFFECTS OF FOOD ADDITIVE SODIUM BENZOATE ON OXIDATIVE STRESS AND GENOTOXICITY

İlter İlhan; Duygu Doguc; Muhammet Yusuf Tepebaşı; Okan Sancer; Halil İbrahim Büyükbayram

Makale detayları

INVESTIGATION OF THE EFFECTS OF FOOD ADDITIVE SODIUM BENZOATE ON OXIDATIVE STRESS AND GENOTOXICITY

Yazarlar:

İlter İlhan | Duygu Doguc | Muhammet Yusuf Tepebaşı | Okan Sancer | Halil İbrahim Büyükbayram

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.276-283

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001299099

pISSN:

1300-7416

Anahtar kelimeler:

Özet:

 Objective Recently, there has been a significant increase in the use of food additives. Generation of possible side effects depending on the long-term consumption of these substances from birth to death has gained importance in humans. In our study, we aimed to evaluate the exposure to sodium benzoate in terms of oxidative stress and genotoxicity, especially in prepubertal rats. Material and Method In our study, 42 male rats 4 weeks-old divided into three groups as the control n14, experiment 1 sodium benzoate Acceptable Daily Intake ADI dose, n14 and experiment 2 sodium benzoate No Advers Effect Level NOAELdose, n14 groups and sodium benzoate was administration by oral gavage for 6 weeks. At the end of the study, rats were sacrified and biochemical parameters glucose, creatinine, AST, ALT, ALP, lipid profile, total protein, albumin, total oxidant status TOS, total antioxidant status TAS, ischemia modified albumin IMA were measured, and DNA damage was evaluated by Comet analysis. Oxidative stress index OSI was calculated from the TOS and TAS parameters. Results ALT and creatinine levels were increased with the NOAEL dosage of sodium benzoate. In addition, TOS, IMA and OSI levels were elevated, while TAS levels were decreased in the NOAEL group. Also, NOAEL dose of sodium benzoate significantly caused DNA damage. Conclusions In our study, no adverse effect was observed in the administration of sodium benzoate at the dose of ADI in prepubertal rats. On the other hand, we found that NOAEL dose of sodium benzoate impairs kidney and liver functions and causes oxidative stress and genotoxicity.

 Objective Recently, there has been a significant increase in the use of food additives. Generation of possible side effects depending on the long-term consumption of these substances from birth to death has gained importance in humans. In our study, we aimed to evaluate the exposure to sodium benzoate in terms of oxidative stress and genotoxicity, especially in prepubertal rats. Material and Method In our study, 42 male rats 4 weeks-old divided into three groups as the control n14, experiment 1 sodium benzoate Acceptable Daily Intake ADI dose, n14 and experiment 2 sodium benzoate No Advers Effect Level NOAELdose, n14 groups and sodium benzoate was administration by oral gavage for 6 weeks. At the end of the study, rats were sacrified and biochemical parameters glucose, creatinine, AST, ALT, ALP, lipid profile, total protein, albumin, total oxidant status TOS, total antioxidant status TAS, ischemia modified albumin IMA were measured, and DNA damage was evaluated by Comet analysis. Oxidative stress index OSI was calculated from the TOS and TAS parameters. Results ALT and creatinine levels were increased with the NOAEL dosage of sodium benzoate. In addition, TOS, IMA and OSI levels were elevated, while TAS levels were decreased in the NOAEL group. Also, NOAEL dose of sodium benzoate significantly caused DNA damage. Conclusions In our study, no adverse effect was observed in the administration of sodium benzoate at the dose of ADI in prepubertal rats. On the other hand, we found that NOAEL dose of sodium benzoate impairs kidney and liver functions and causes oxidative stress and genotoxicity.

 Objective Recently, there has been a significant increase in the use of food additives. Generation of possible side effects depending on the long-term consumption of these substances from birth to death has gained importance in humans. In our study, we aimed to evaluate the exposure to sodium benzoate in terms of oxidative stress and genotoxicity, especially in prepubertal rats. Material and Method In our study, 42 male rats 4 weeks-old divided into three groups as the control n14, experiment 1 sodium benzoate Acceptable Daily Intake ADI dose, n14 and experiment 2 sodium benzoate No Advers Effect Level NOAELdose, n14 groups and sodium benzoate was administration by oral gavage for 6 weeks. At the end of the study, rats were sacrified and biochemical parameters glucose, creatinine, AST, ALT, ALP, lipid profile, total protein, albumin, total oxidant status TOS, total antioxidant status TAS, ischemia modified albumin IMA were measured, and DNA damage was evaluated by Comet analysis. Oxidative stress index OSI was calculated from the TOS and TAS parameters. Results ALT and creatinine levels were increased with the NOAEL dosage of sodium benzoate. In addition, TOS, IMA and OSI levels were elevated, while TAS levels were decreased in the NOAEL group. Also, NOAEL dose of sodium benzoate significantly caused DNA damage. Conclusions In our study, no adverse effect was observed in the administration of sodium benzoate at the dose of ADI in prepubertal rats. On the other hand, we found that NOAEL dose of sodium benzoate impairs kidney and liver functions and causes oxidative stress and genotoxicity.

 Amaç Son yıllarda gıda katkı maddelerinin kullanımında belirgin artış vardır. Doğumdan ölüme kadar maruz kalınan bu maddelerin, insanlarda uzun süreli tüketime bağlı olarak oluşturabileceği yan etkiler oldukça önem kazanmıştır. Çalışmamızda özellikle puberte öncesi sıçanlarda sodyum benzoata maruziyetin oksidatif stres ve genotoksisite açılarından değerlendirilmesini amaçladık. Gereç ve Yöntem Kırk iki adet, 4 haftalık erkek sıçan, kontrol n14, deney 1 Acceptable Daily Intake ADI dozunda sodyum benzoat, n14 ve deney 2 No Advers Effect Level NOAEL dozunda sodyum benzoat, n14 grupları şeklinde 3 gruba ayrılmıştır. Altı hafta süresince oral gavaj uygulanmıştır. Çalışma sonunda sakrifiye edilen sıçanların kan örneklerinden biyokimyasal parametreler glukoz, kreatinin, AST, ALT, ALP, lipid profili, total protein, albümin, total oksidan status TOS, total antioksidan status TAS, iskemi modifiye albümin İMA ölçülmüş ve Comet yöntemi ile DNA hasarı değerlendirilmiştir. Ölçülen TOS ve TAS parametrelerinden oksidatif stres indeksi OSİ hesaplanmıştır. Bulgular NOAEL dozu sodyum benzoat uygulanan sıçanlarda ALT ve kreatinin seviyeleri yükselmiştir. Ayrıca TOS, IMA ve OSİ seviyeleri NOAEL grubunda yüksek iken TAS seviyeleri düşük bulunmuştur. Bunlara ek olarak NOAEL dozunda sodyum benzoat DNA hasarına yol açmıştır. Sonuç Çalışmamızda, prepubertal dönem sıçanlarda ADI dozunda sodyum benzoat uygulamasının, olumsuz bir etkisi gözlemlenmemiştir. Diğer taraftan NOAEL dozunda sodyum benzoat uygulamasının böbrek ve karaciğer fonksiyonlarını bozduğunu ve oksidatif stres ile genotoksisiteye yol açabileceği ortaya konmuştur.

Makale detayları

INVESTIGATION OF THE EFFECTS OF FOOD ADDITIVE SODIUM BENZOATE ON OXIDATIVE STRESS AND GENOTOXICITY

Yazarlar:

İlter İlhan | Duygu Doguc | Muhammet Yusuf Tepebaşı | Okan Sancer | Halil İbrahim Büyükbayram

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.276-283

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001299099

pISSN:

1300-7416

Comet

Food additive

Oxidative stress

Sodium benzoateComet

Gıda katkı maddesi

Oksidatif stres

Sodyum benzoat

Sayı

Makale detayları

Yazarlar: İlter İlhan | Duygu Doguc | Muhammet Yusuf Tepebaşı | Okan Sancer | Halil İbrahim Büyükbayram

Yayıncı: Süleyman Demirel University

Ana Başlıklar: Clinical Sciences

Dergi: Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.276-283

Belge Tipi: Makale

Yayın Tarihi: 9/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001299099

pISSN: 1300-7416

Sayı

Yazarlar:

İlter İlhan | Duygu Doguc | Muhammet Yusuf Tepebaşı | Okan Sancer | Halil İbrahim Büyükbayram

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.276-283

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001299099

pISSN:

1300-7416