ASSESSMENT OF IN VITRO GENOTOXICITY AND ANTIGENOTOXICITY OF LUTEOLIN BY USING COMET ASSAY

Esra ERIKEL; Deniz YÜZBAŞIOĞLU; Fatma ÜNAL

Makale detayları

ASSESSMENT OF IN VITRO GENOTOXICITY AND ANTIGENOTOXICITY OF LUTEOLIN BY USING COMET ASSAY

Yazarlar:

Esra ERIKEL | Deniz YÜZBAŞIOĞLU | Fatma ÜNAL

Yayıncı:

Engin TUTKUN

Dergi:

The Turkish Journal Of Occupational / Environmental Medicine and Safety 2017 Vol.Volume 2 Issue İssue 1 (1), pp.1-174

Belge Tipi:

Makale

Yayın Tarihi:

2/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000835360

Sıklık:

Üç Aylık

eISSN:

2149-4711

pISSN:

2149-4711

Özet:

 


Luteolin is a
flavone which occurs in medicinal plants as well as in some vegetables and
spices. Celery, green pepper, carrots, olives and artichokes are major
nutritional luteolin sources. Many studies have demonstrated that luteolin has
numerous biological activities such as anti-oxidant, anti-inflammatory,
cardioprotective, anti-diabetic, anti-allergic and anti-cancer. The purpose of
this study is to investigate genotoxic and antigenotoxic effect of luteolin
against H2O2 induced DNA damage by using comet assay in
human lymphocyte in vitro. The lymphocytes isolated from 2 healthy volunteer (1
male and 1 female) were incubated with five different concentrations of
luteolin (0.39, 0.78, 1.56, 3.12 and 6.25 µg/mL) alone and simultaneously with
H2O2 (100 mM) at 37oC for 1 hour. A negative
(distilled water), a solvent (50% methanol) and a positive control (H2O2) were
also maintained. A total of 200 cells were evaluated per concentration for tail
intensity (%), tail length (μm), and tail moment by using Comet Assay IV,
Perceptive Instruments Ltd., UK. Luteolin alone did not induce significant DNA
damage in all the concentrations compared to negative and solvent controls.
Luteolin+ H2O2 treatment significantly reduced DNA damage
at all the concentrations compared to H2O2 treatment
alone (positive control) for all comet parameters. Our results have indicated
that luteolin exhibited chemopreventive activity against DNA damage induced by
H2O2 which has oxidative effects.

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Makale detayları

ASSESSMENT OF IN VITRO GENOTOXICITY AND ANTIGENOTOXICITY OF LUTEOLIN BY USING COMET ASSAY

Yazarlar: Esra ERIKEL | Deniz YÜZBAŞIOĞLU | Fatma ÜNAL

Yayıncı: Engin TUTKUN

Dergi: The Turkish Journal Of Occupational / Environmental Medicine and Safety 2017 Vol.Volume 2 Issue İssue 1 (1), pp.1-174

Belge Tipi: Makale

Yayın Tarihi: 2/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000835360

Sıklık: Üç Aylık

eISSN: 2149-4711

pISSN: 2149-4711

Sayı

Yazarlar:

Esra ERIKEL | Deniz YÜZBAŞIOĞLU | Fatma ÜNAL

Yayıncı:

Engin TUTKUN

Dergi:

The Turkish Journal Of Occupational / Environmental Medicine and Safety 2017 Vol.Volume 2 Issue İssue 1 (1), pp.1-174

Belge Tipi:

Makale

Yayın Tarihi:

2/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000835360

Sıklık:

Üç Aylık

eISSN:

2149-4711

pISSN:

2149-4711