Isolation and Characterization of Bacillus spp. Producing Indole-3-Acetic Acid (IAA) and Solubilizing Inorganic Phosphate from the Rhizosphere of Medicinal Sage (Salvia officinalis)

Murat Güler

Makale detayları

Isolation and Characterization of Bacillus spp. Producing Indole-3-Acetic Acid (IAA) and Solubilizing Inorganic Phosphate from the Rhizosphere of Medicinal Sage (Salvia officinalis)

Yazarlar:

Murat Güler

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Medicinal and Aromatic Plants | Soil Sciences and Plant Nutrition (Other)

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 3, pp.716-726

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001355246

pISSN:

2148-3647

Anahtar kelimeler:

Özet:

 The production of indole-3-acetic acid IAA is a key characteristic of plant growth-promoting bacteria PGPR. This hormone, synthesized by PGPR in the rhizosphere, is responsible for the division, elongation, and differentiation of plant cells and plays a crucial role in various physiological mechanisms in plants. 21 bacterial isolates were obtained from the rhizosphere of medicinal sage Salvia officinalis L. in this study. This study aimed to characterize the indigenous bacterial community in the rhizosphere of S. officinalis and evaluate their potential as microbial fertilizers, focusing on their IAA production and phosphate solubilization capabilities. Among these isolates, 9 were identified as Bacillus sp. through morphological and biochemical tests as well as the MALDI-TOF MS method. Furthermore,9 isolates, 5 BCM-3, BTM-1, BN-5, BCM-4, and BGM-13 demonstrated the ability to dissolve inorganic phosphate, while 6 BCM-3, BCM-4, BSM-1, BSM-2, BTM-1, and BN-5 produced indole-3-acetic acid IAA in varying percentages. IAA production was assessed in nutrient broth NB medium supplemented with 0.2 L-tryptophan and measured at different incubation times. The results revealed that maximum IAA production by Bacillus cereus BCM-3 and BCM-4 was achieved after 3 days of incubation, with the highest production observed in BCM-3 129.8 µg ml¹. Furthermore, B. cereus BCM-3 and BCM-4 exhibited the highest inorganic phosphate solubilization performance among all tested Bacillus isolates. Our findings demonstrated that BCM-3 and BCM-4, isolated from the rhizosphere of medicinal sage, have significant potential for use as microbial fertilizer applications due to their high IAA production and inorganic phosphate solubilization abilities.

 The production of indole-3-acetic acid IAA is a key characteristic of plant growth-promoting bacteria PGPR. This hormone, synthesized by PGPR in the rhizosphere, is responsible for the division, elongation, and differentiation of plant cells and plays a crucial role in various physiological mechanisms in plants. 21 bacterial isolates were obtained from the rhizosphere of medicinal sage Salvia officinalis L. in this study. This study aimed to characterize the indigenous bacterial community in the rhizosphere of S. officinalis and evaluate their potential as microbial fertilizers, focusing on their IAA production and phosphate solubilization capabilities. Among these isolates, 9 were identified as Bacillus sp. through morphological and biochemical tests as well as the MALDI-TOF MS method. Furthermore,9 isolates, 5 BCM-3, BTM-1, BN-5, BCM-4, and BGM-13 demonstrated the ability to dissolve inorganic phosphate, while 6 BCM-3, BCM-4, BSM-1, BSM-2, BTM-1, and BN-5 produced indole-3-acetic acid IAA in varying percentages. IAA production was assessed in nutrient broth NB medium supplemented with 0.2 L-tryptophan and measured at different incubation times. The results revealed that maximum IAA production by Bacillus cereus BCM-3 and BCM-4 was achieved after 3 days of incubation, with the highest production observed in BCM-3 129.8 µg ml¹. Furthermore, B. cereus BCM-3 and BCM-4 exhibited the highest inorganic phosphate solubilization performance among all tested Bacillus isolates. Our findings demonstrated that BCM-3 and BCM-4, isolated from the rhizosphere of medicinal sage, have significant potential for use as microbial fertilizer applications due to their high IAA production and inorganic phosphate solubilization abilities.

 The production of indole-3-acetic acid IAA is a key characteristic of plant growth-promoting bacteria PGPR. This hormone, synthesized by PGPR in the rhizosphere, is responsible for the division, elongation, and differentiation of plant cells and plays a crucial role in various physiological mechanisms in plants. 21 bacterial isolates were obtained from the rhizosphere of medicinal sage Salvia officinalis L. in this study. This study aimed to characterize the indigenous bacterial community in the rhizosphere of S. officinalis and evaluate their potential as microbial fertilizers, focusing on their IAA production and phosphate solubilization capabilities. Among these isolates, 9 were identified as Bacillus sp. through morphological and biochemical tests as well as the MALDI-TOF MS method. Furthermore,9 isolates, 5 BCM-3, BTM-1, BN-5, BCM-4, and BGM-13 demonstrated the ability to dissolve inorganic phosphate, while 6 BCM-3, BCM-4, BSM-1, BSM-2, BTM-1, and BN-5 produced indole-3-acetic acid IAA in varying percentages. IAA production was assessed in nutrient broth NB medium supplemented with 0.2 L-tryptophan and measured at different incubation times. The results revealed that maximum IAA production by Bacillus cereus BCM-3 and BCM-4 was achieved after 3 days of incubation, with the highest production observed in BCM-3 129.8 µg ml¹. Furthermore, B. cereus BCM-3 and BCM-4 exhibited the highest inorganic phosphate solubilization performance among all tested Bacillus isolates. Our findings demonstrated that BCM-3 and BCM-4, isolated from the rhizosphere of medicinal sage, have significant potential for use as microbial fertilizer applications due to their high IAA production and inorganic phosphate solubilization abilities.

 İndol-3-asetik asit IAA üretimi, bitki büyümesini teşvik eden bakterilerin PGPR temel bir özelliğidir. Rizosferde bitki gelişimini teşvik eden rizobakteri PGPR tarafından sentezlenen bu hormon, bitki hücrelerinin bölünmesinden, uzamasından ve farklılaşmasından sorumludur ve bitkilerde çeşitli fizyolojik mekanizmalarda önemli bir rol oynar. Bu çalışmada, tıbbi adaçayı Salvia officinalis L. rizosferinden 21 bakteri izolatı elde edilmiştir. Bu izolatlardan 9 tanesinin morfolojik, biyokimyasal testler ve MALDI TOF MS yöntemi ile Bacillus sp. olduğu belirlenmiştir. Dokuz izolat arasından beş tanesinin BCM-3, BTM-1, BN-5, BCM-4 ve BGM-13 inorganik fosfat çözdüğü, 6 tanesinin BCM-3, BCM-4, BSM-1, BSM-2, BTM-1 ve BN-5 farklı oranlarda IAA ürettiği belirlenmiştir. IAA üretimi,  0,2 L-triptofan eklenmiş nutrient broth NB ortamında farklı inkübasyon sürelerinde gerçekleşmiştir. Sonuçlar, Bacillus cereus BCM-3 ve BCM-4ün maksimum IAA üretiminin 3 günlük inkübasyon süresinde gerçekleştiğini ortaya koymuştur. En iyi IAA üretimi, 3 günlük inkübasyon süresinde B. cereus BCM-3ten 129.8 µg ml¹ elde edilmiştir. Ayrıca, B. cereus BCM-3 ve BCM-4 izolatlarının test edilen tüm Basillus izolatları arasında en iyi inorganik fosfat çözme performansını sergilemiştir. Bulgularımız, tıbbi adaçayı topraklarından izole edilen BCM-3 ve BCM-4 izolatlarının yüksek IAA üretimi ve inorganik fosfat çözme yetenekleri nedeniyle mikrobiyal gübre uygulamaları için önemli bir potansiyele sahip olduğunu göstermiştir. Bu çalışma, Salvia officinalis adaçayı rizosferindeki yerel bakteriyel topluluğu karakterize etmeyi, IAA üretme ve inorganik fosfat çözme yetenekleri ile mikrobiyal gübre potansiyellerini değerlendirmeyi amaçlamıştır.

Makale detayları

Isolation and Characterization of Bacillus spp. Producing Indole-3-Acetic Acid (IAA) and Solubilizing Inorganic Phosphate from the Rhizosphere of Medicinal Sage (Salvia officinalis)

Yazarlar:

Murat Güler

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Medicinal and Aromatic Plants | Soil Sciences and Plant Nutrition (Other)

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 3, pp.716-726

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001355246

pISSN:

2148-3647

Medicinal Sage

Bacillus spp.

PGPR

microbial fertilizersTıbbi adaçayı

Bacillus spp.

PGPR

mikrobiyal gübre

Sayı

Makale detayları

Yazarlar: Murat Güler

Yayıncı: Mevlüt AKÇURA

Ana Başlıklar: Medicinal and Aromatic Plants | Soil Sciences and Plant Nutrition (Other)

Dergi: Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 3, pp.716-726

Belge Tipi: Makale

Yayın Tarihi: 7/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001355246

pISSN: 2148-3647

Sayı

Yazarlar:

Murat Güler

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Medicinal and Aromatic Plants | Soil Sciences and Plant Nutrition (Other)

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 3, pp.716-726

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001355246

pISSN:

2148-3647