MORPHOMETRIC EFFECT OF METHANOLONE ENANTHATE ON SCAPULA IN ADOLESCENT RATS

Mehmet ÖZDEMİR; İbrahim BOZKURT; Sadullah BAHAR; Özgür ÖZDEMİR

doi:https://doi.org/10.22282/ojrs.2020.60

Makale detayları

MORPHOMETRIC EFFECT OF METHANOLONE ENANTHATE ON SCAPULA IN ADOLESCENT RATS

Yazarlar:

Mehmet ÖZDEMİR | İbrahim BOZKURT | Sadullah BAHAR | Özgür ÖZDEMİR

Yayıncı:

Metin YAMAN

Dergi:

The Online Journal of Recreation and Sports 2020 Vol.9 Issue 1, pp.22-29

Belge Tipi:

Makale

Yayın Tarihi:

12/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000668076

Sıklık:

Üç Aylık

eISSN:

2146-9598

pISSN:

2146-9598

DOI:

https://doi.org/10.22282/ojrs.2020.60

Özet:

 

This study was carried out to determine the effect of Methenolone enanthate  ME  on the doping list on male and female rat scapula during puberty. In this study, three groups  ME, FY, K  were created for males and females, each containing 8 rats, and a total of 48 rats were used. Methenolone enanthate  Primobolan Depot ® Bayer  was administered to the experimental groups at a dose of 0.5 mg / kg  0.5 ml, diluted in peanut oil  5 times a week intraperitoneally for 4 weeks. All rats were euthanized under thiopental  40 mg / kg  anesthesia after 4 weeks. After necropsy, both scapula bones were dissected and macerated. At the end of this process, the bones were dried in a 37 C oven for 24 hours. Morphometric measurements of right and left scapula bones of male and female rats in each group were opened in the semiautomatic image analysis program imageJ  Ver. 1.44, National Institutes of Health . After calibrating the images, the height of the scapula and then the largest distance perpendicular to the height axis were measured by using the freehand line feature of the program  Fig 1 . Area measurements of the medial surfaces of the bones were made using the point counting method  Başoglu et  al, 2007, Akosman and Özdemir, 2010; Bolat et al, 2011 .  For this purpose, by using grid function of imagej program   plugins-analyze-grid , grids with 2 mm between two  points and 4 mm2 of area represented by each point were  created. These grids were superimposed on the bone  images and the points falling on the bone surface were  counted  Fig 2 . The number of points obtained was  multiplied by the area represented by each point and the  bone surface areas of the animals in the groups were  calculated separately in mm2.vAs a result, it was found  that the effect of ME on rat scapula height, width and  surface area was similar to other AAS’s suppressed bone  development in male pubers and encouraged bone  development in females. The negative effects of the  commonly used doping agents on bones in males in the  medium and long term have been tried to be demonstrated  with rat models.

Makale detayları

MORPHOMETRIC EFFECT OF METHANOLONE ENANTHATE ON SCAPULA IN ADOLESCENT RATS

Yazarlar: Mehmet ÖZDEMİR | İbrahim BOZKURT | Sadullah BAHAR | Özgür ÖZDEMİR

Yayıncı: Metin YAMAN

Dergi: The Online Journal of Recreation and Sports 2020 Vol.9 Issue 1, pp.22-29

Belge Tipi: Makale

Yayın Tarihi: 12/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000668076

Sıklık: Üç Aylık

eISSN: 2146-9598

pISSN: 2146-9598

DOI: https://doi.org/10.22282/ojrs.2020.60

Sayı

Yazarlar:

Mehmet ÖZDEMİR | İbrahim BOZKURT | Sadullah BAHAR | Özgür ÖZDEMİR

Yayıncı:

Metin YAMAN

Dergi:

The Online Journal of Recreation and Sports 2020 Vol.9 Issue 1, pp.22-29

Belge Tipi:

Makale

Yayın Tarihi:

12/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000668076

Sıklık:

Üç Aylık

eISSN:

2146-9598

pISSN:

2146-9598

DOI:

https://doi.org/10.22282/ojrs.2020.60