Effect of cell-conditioned media on biomass production of Leishmania parasites

Melahat BAGIROVA; Rabia ÇAKIR KOÇ; Adil ALLAHVERDİYEV; Melike ERSÖZ

Makale detayları

Effect of cell-conditioned media on biomass production of Leishmania parasites

Yazarlar:

Melahat BAGIROVA | Rabia ÇAKIR KOÇ | Adil ALLAHVERDİYEV | Melike ERSÖZ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 6, pp.653-657

Belge Tipi:

Makale

Yayın Tarihi:

5/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812546

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

A large amount of parasite biomass production necessitates a higher volume of culture media, and this further increases the overall cost. For this reason, novel and alternative culture supplementary materials have been continuously investigated. The aim of this study was therefore to evaluate the effect of cell-conditioned media, collected as waste material during routine cell culture, on the growth and development of Leishmania parasites in vitro. In this study, for the first time, we show that cell-conditioned medium obtained from MCF-7 culture increased the growth of Leishmania parasites in vitro. These results show that the metabolites that are present in cell-conditioned media may play an important role in in vitro parasite culture. Cell-conditioned media may be used as an economic tool for isolation, cultivation, and obtaining biomass of Leishmania parasites in laboratory conditions.

 

A large amount of parasite biomass production necessitates a higher volume of culture media, and this further increases the overall cost. For this reason, novel and alternative culture supplementary materials have been continuously investigated. The aim of this study was therefore to evaluate the effect of cell-conditioned media, collected as waste material during routine cell culture, on the growth and development of Leishmania parasites in vitro. In this study, for the first time, we show that cell-conditioned medium obtained from MCF-7 culture increased the growth of Leishmania parasites in vitro. These results show that the metabolites that are present in cell-conditioned media may play an important role in in vitro parasite culture. Cell-conditioned media may be used as an economic tool for isolation, cultivation, and obtaining biomass of Leishmania parasites in laboratory conditions.

Makale detayları

Effect of cell-conditioned media on biomass production of Leishmania parasites

Yazarlar:

Melahat BAGIROVA | Rabia ÇAKIR KOÇ | Adil ALLAHVERDİYEV | Melike ERSÖZ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 6, pp.653-657

Belge Tipi:

Makale

Yayın Tarihi:

5/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812546

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Culture

medium

Leishmania

large-scale

biomass

Sayı

Makale detayları

Effect of cell-conditioned media on biomass production of Leishmania parasites

Yazarlar: Melahat BAGIROVA | Rabia ÇAKIR KOÇ | Adil ALLAHVERDİYEV | Melike ERSÖZ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2012 Vol.36 Issue 6, pp.653-657

Belge Tipi: Makale

Yayın Tarihi: 5/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000812546

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Culture

medium

Leishmania

large-scale

biomass

Sayı

Yazarlar:

Melahat BAGIROVA | Rabia ÇAKIR KOÇ | Adil ALLAHVERDİYEV | Melike ERSÖZ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 6, pp.653-657

Belge Tipi:

Makale

Yayın Tarihi:

5/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812546

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152