Cyclic Voltammetry Based Microfluidic System Design and Analysis

N.A.

Makale detayları

Cyclic Voltammetry Based Microfluidic System Design and Analysis

Yazarlar:

N.A.

Yayıncı:

Cukurova University

Ana Başlıklar:

Mühendislik

Dergi:

Çukurova Üniversitesi Mühendislik Fakültesi Dergisi Vol.38 Issue 1, pp.185-194

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001287009

pISSN:

2757-9255

Anahtar kelimeler:

Özet:

 In this study, the determination of glucose in the fluid was made by monitoring the redox reactions occurring on the biosensor integrated into a microfluidic system by cyclic voltammetry method. Comsol Multiphysics v3.5a was used for the design and finite element analysis of the microchannel integrated biosensor. In this context, an electrode was placed on a microchannel surface with a height of 300 µm and a length of 1500 µm. Mathematical modeling was primarily performed without fluid flow through the microchannel. A cyclic voltammetry-based microfluidic system was designed by continuing the analyzes by defining a liquid inlet to the microchannel inlet under laminar flow conditions. The efficiency of the biosensor was tested at different glucose values such as 0.25mM, 0.5mM, 1mM, 2mM, 4mM and 8mM. In order to determine the effect of the flow rate of the laminar flow defined at the entrance of the microchannel on the response from the biosensor, analyzes were made at different flow rates such as 25 µlmin, 50 µlmin, 100 µlmin and 200 µlmin. In order to examine the effect of electrode length on the response from cyclic voltammetry, the electrode lengths were changed to 100µm, 200µm and 300µm. In this study, it was observed that the maximum current obtained from cyclic voltammetry increased in the presence of laminar flow. Simulations have shown that increasing the fluid flow rate increases the efficiency of the biosensor. As a result, the feasibility of glucose determination with a cyclic voltammetry-based microfluidic system has been demonstrated with this study.

 In this study, the determination of glucose in the fluid was made by monitoring the redox reactions occurring on the biosensor integrated into a microfluidic system by cyclic voltammetry method. Comsol Multiphysics v3.5a was used for the design and finite element analysis of the microchannel integrated biosensor. In this context, an electrode was placed on a microchannel surface with a height of 300 µm and a length of 1500 µm. Mathematical modeling was primarily performed without fluid flow through the microchannel. A cyclic voltammetry-based microfluidic system was designed by continuing the analyzes by defining a liquid inlet to the microchannel inlet under laminar flow conditions. The efficiency of the biosensor was tested at different glucose values such as 0.25mM, 0.5mM, 1mM, 2mM, 4mM and 8mM. In order to determine the effect of the flow rate of the laminar flow defined at the entrance of the microchannel on the response from the biosensor, analyzes were made at different flow rates such as 25 µlmin, 50 µlmin, 100 µlmin and 200 µlmin. In order to examine the effect of electrode length on the response from cyclic voltammetry, the electrode lengths were changed to 100µm, 200µm and 300µm. In this study, it was observed that the maximum current obtained from cyclic voltammetry increased in the presence of laminar flow. Simulations have shown that increasing the fluid flow rate increases the efficiency of the biosensor. As a result, the feasibility of glucose determination with a cyclic voltammetry-based microfluidic system has been demonstrated with this study.

 In this study, the determination of glucose in the fluid was made by monitoring the redox reactions occurring on the biosensor integrated into a microfluidic system by cyclic voltammetry method. Comsol Multiphysics v3.5a was used for the design and finite element analysis of the microchannel integrated biosensor. In this context, an electrode was placed on a microchannel surface with a height of 300 µm and a length of 1500 µm. Mathematical modeling was primarily performed without fluid flow through the microchannel. A cyclic voltammetry-based microfluidic system was designed by continuing the analyzes by defining a liquid inlet to the microchannel inlet under laminar flow conditions. The efficiency of the biosensor was tested at different glucose values such as 0.25mM, 0.5mM, 1mM, 2mM, 4mM and 8mM. In order to determine the effect of the flow rate of the laminar flow defined at the entrance of the microchannel on the response from the biosensor, analyzes were made at different flow rates such as 25 µlmin, 50 µlmin, 100 µlmin and 200 µlmin. In order to examine the effect of electrode length on the response from cyclic voltammetry, the electrode lengths were changed to 100µm, 200µm and 300µm. In this study, it was observed that the maximum current obtained from cyclic voltammetry increased in the presence of laminar flow. Simulations have shown that increasing the fluid flow rate increases the efficiency of the biosensor. As a result, the feasibility of glucose determination with a cyclic voltammetry-based microfluidic system has been demonstrated with this study.

 Bu çalışmada bir mikroakışkan sisteme entegre edilmiş biyosensörün üzerinde oluşan redoks tepkimeler çevrimsel voltammetri yöntemiyle izlenerek akışkan içerisindeki glukozun tayini yapılmıştır. Mikrokanala entegre biyosensörün tasarımı ve sonlu elemanlar analizi için Comsol Multiphysics v3.5a kullanılmıştır. Bu bağlamda, yüksekliği 300 µm ve uzunluğu 1500 µm olan bir mikrokanal yüzeyine elektrot yerleştirilmiştir. Matematiksel modelleme öncelikli olarak mikrokanal boyunca sıvı akışı olmadan gerçekleştirilmiştir. Analizlere mikrokanal girişine laminer akış koşulları altında bir sıvı girişi tanımlanarak devam edilerek çevrimsel voltammetri tabanlı bir mikroakışkan sistem tasarımı yapılmıştır. Biyosensörün etkinliği 0.25mM, 0.5mM, 1mM, 2mM, 4mM ve 8mM gibi farklı glukoz değerlerinde test edilmiştir. Mikrokanalın girişine tanımlanan laminar akışın debisinin biyosensörden alınan yanıta etkisinin belirlenmesi için 25 µldk, 50 µldk, 100 µldk ve 200 µldk gibi farklı debi değerlerinde çözümlemeler yapılmıştır. Elektrot uzunluğunun çevrimsel voltammetriden alınan cevaba etkisinin incelebilmesi için elektrot uzunluğu 100µm, 200µm ve 300µm olarak değiştirilmiştir. Bu çalışmada laminer akış varlığında çevrimsel voltammetriden elde edilen maksimum akımın yükseldiği gözlemlenmiştir. Simülasyonlar, sıvı akış debisinin arttırılmasının biyosensörün etkinliğini arttırdığını göstermiştir. Sonuç olarak, bu çalışma ile çevrimsel voltammetri tabanlı bir mikroakışkan sistem ile glukoz tayininin yapılabilirliği ortaya konulmuştur.

Makale detayları

Cyclic Voltammetry Based Microfluidic System Design and Analysis

Yazarlar:

N.A.

Yayıncı:

Cukurova University

Ana Başlıklar:

Mühendislik

Dergi:

Çukurova Üniversitesi Mühendislik Fakültesi Dergisi Vol.38 Issue 1, pp.185-194

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001287009

pISSN:

2757-9255

Glucose determination

Cyclic voltammetry

Biosensor

Finite element analysis

Glukoz Tayini

Çevrimsel voltammetri

Biyosensör

Sonlu elemanlar analizi

Sayı

Makale detayları

Yazarlar: N.A.

Yayıncı: Cukurova University

Ana Başlıklar: Mühendislik

Dergi: Çukurova Üniversitesi Mühendislik Fakültesi Dergisi Vol.38 Issue 1, pp.185-194

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001287009

pISSN: 2757-9255

Sayı

Yazarlar:

N.A.

Yayıncı:

Cukurova University

Ana Başlıklar:

Mühendislik

Dergi:

Çukurova Üniversitesi Mühendislik Fakültesi Dergisi Vol.38 Issue 1, pp.185-194

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001287009

pISSN:

2757-9255