Analysis of Frozen Bull Spermatozoa After Direct Washing with The Brackett Oliphant Medium

Numan Akyol; Abdulkadir Kaya

Makale detayları

Analysis of Frozen Bull Spermatozoa After Direct Washing with The Brackett Oliphant Medium

Yazarlar:

Numan Akyol | Abdulkadir Kaya

Yayıncı:

Kastamonu University

Ana Başlıklar:

Veterinary Sciences (Other)

Dergi:

Veterinary Journal of Kastamonu University Vol.3 Issue 1, pp.9-16

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001321532

pISSN:

2822-4922

Anahtar kelimeler:

Özet:

 Aim to study: The purpose of this study was to investigate the effects of the method of direct washing with the Brackett Oliphant BO medium which is used for purposes of in vitro fertilization on some spermatological parameters in Holstein and Brown Swiss bulls. Material and methods: The study used cryopreserved sperm obtained from the Holstein n5 and Brown Swiss n5 breeds. After this procedure, Computer Assisted Sperm Analysis CASA and oxidative status analyses were conducted both before and after direct washing method with BO. The data were analyzed using the statistical methods of paired-samples t-test and independent-samples t-test. Results: After direct washing with BO, the total motility in the Brown Swiss group decreased from 62.67 to 33.33 P0.011. After washing with BO, the total antioxidant level decreased from 1.45 to 0.11 in Group 1 P  0.000 and from 0.83 to 0.09 in Group 2 P0.000. Additionally, the total oxidant level increased from 5.54 to 5.70 in Group 1 P  0.024 and from 4.94 to 5.12 in Group 2 P0.019. Conclusion: According to the findings, the direct washing method with BO can negatively affect Brown Swiss spermatozoa motility. Additionally, after washing, the antioxidant level significantly decreases, and the oxidant levels increase due to oxidative stress.

 Aim to study: The purpose of this study was to investigate the effects of the method of direct washing with the Brackett Oliphant BO medium which is used for purposes of in vitro fertilization on some spermatological parameters in Holstein and Brown Swiss bulls. Material and methods: The study used cryopreserved sperm obtained from the Holstein n5 and Brown Swiss n5 breeds. After this procedure, Computer Assisted Sperm Analysis CASA and oxidative status analyses were conducted both before and after direct washing method with BO. The data were analyzed using the statistical methods of paired-samples t-test and independent-samples t-test. Results: After direct washing with BO, the total motility in the Brown Swiss group decreased from 62.67 to 33.33 P0.011. After washing with BO, the total antioxidant level decreased from 1.45 to 0.11 in Group 1 P  0.000 and from 0.83 to 0.09 in Group 2 P0.000. Additionally, the total oxidant level increased from 5.54 to 5.70 in Group 1 P  0.024 and from 4.94 to 5.12 in Group 2 P0.019. Conclusion: According to the findings, the direct washing method with BO can negatively affect Brown Swiss spermatozoa motility. Additionally, after washing, the antioxidant level significantly decreases, and the oxidant levels increase due to oxidative stress.

 Aim to study: The purpose of this study was to investigate the effects of the method of direct washing with the Brackett Oliphant BO medium which is used for purposes of in vitro fertilization on some spermatological parameters in Holstein and Brown Swiss bulls. Material and methods: The study used cryopreserved sperm obtained from the Holstein n5 and Brown Swiss n5 breeds. After this procedure, Computer Assisted Sperm Analysis CASA and oxidative status analyses were conducted both before and after direct washing method with BO. The data were analyzed using the statistical methods of paired-samples t-test and independent-samples t-test. Results: After direct washing with BO, the total motility in the Brown Swiss group decreased from 62.67 to 33.33 P0.011. After washing with BO, the total antioxidant level decreased from 1.45 to 0.11 in Group 1 P  0.000 and from 0.83 to 0.09 in Group 2 P0.000. Additionally, the total oxidant level increased from 5.54 to 5.70 in Group 1 P  0.024 and from 4.94 to 5.12 in Group 2 P0.019. Conclusion: According to the findings, the direct washing method with BO can negatively affect Brown Swiss spermatozoa motility. Additionally, after washing, the antioxidant level significantly decreases, and the oxidant levels increase due to oxidative stress.

 Aim to study: The purpose of this study was to investigate the effects of the method of direct washing with the Brackett Oliphant BO medium which is used for purposes of in vitro fertilization on some spermatological parameters in Holstein and Brown Swiss bulls. Material and methods: The study used cryopreserved sperm obtained from the Holstein n5 and Brown Swiss n5 breeds. After this procedure, Computer Assisted Sperm Analysis CASA and oxidative status analyses were conducted both before and after direct washing method with BO. The data were analyzed using the statistical methods of paired-samples t-test and independent-samples t-test. Results: After direct washing with BO, the total motility in the Brown Swiss group decreased from 62.67 to 33.33 P0.011. After washing with BO, the total antioxidant level decreased from 1.45 to 0.11 in Group 1 P  0.000 and from 0.83 to 0.09 in Group 2 P0.000. Additionally, the total oxidant level increased from 5.54 to 5.70 in Group 1 P  0.024 and from 4.94 to 5.12 in Group 2 P0.019. Conclusion: According to the findings, the direct washing method with BO can negatively affect Brown Swiss spermatozoa motility. Additionally, after washing, the antioxidant level significantly decreases, and the oxidant levels increase due to oxidative stress.

Makale detayları

Analysis of Frozen Bull Spermatozoa After Direct Washing with The Brackett Oliphant Medium

Yazarlar:

Numan Akyol | Abdulkadir Kaya

Yayıncı:

Kastamonu University

Ana Başlıklar:

Veterinary Sciences (Other)

Dergi:

Veterinary Journal of Kastamonu University Vol.3 Issue 1, pp.9-16

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001321532

pISSN:

2822-4922

Brackett-Oliphant

CASA

frozen bull semen

oxidative status

spermatozoa

Sayı

Makale detayları

Analysis of Frozen Bull Spermatozoa After Direct Washing with The Brackett Oliphant Medium

Yazarlar: Numan Akyol | Abdulkadir Kaya

Yayıncı: Kastamonu University

Ana Başlıklar: Veterinary Sciences (Other)

Dergi: Veterinary Journal of Kastamonu University Vol.3 Issue 1, pp.9-16

Belge Tipi: Makale

Yayın Tarihi: 6/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001321532

pISSN: 2822-4922

Brackett-Oliphant

CASA

frozen bull semen

oxidative status

spermatozoa

Sayı

Yazarlar:

Numan Akyol | Abdulkadir Kaya

Yayıncı:

Kastamonu University

Ana Başlıklar:

Veterinary Sciences (Other)

Dergi:

Veterinary Journal of Kastamonu University Vol.3 Issue 1, pp.9-16

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001321532

pISSN:

2822-4922