Investigation of the Neurotoxic Effects of Dimethyl Phthalate and Diisobutyl Phthalate on SH-SY5Y Neuroblastoma Cells

Mehtap Kara; Ayşenur Erdinç; Erkan Gülgen; Yağmur Emre Arıcan; Çiğdem Sevim

Makale detayları

Investigation of the Neurotoxic Effects of Dimethyl Phthalate and Diisobutyl Phthalate on SH-SY5Y Neuroblastoma Cells

Yazarlar:

Mehtap Kara | Ayşenur Erdinç | Erkan Gülgen | Yağmur Emre Arıcan | Çiğdem Sevim

Yayıncı:

Ataturk University

Ana Başlıklar:

Toxicology

Dergi:

Recent Trends in Pharmacology Vol.2 Issue 3, pp.101-108

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001337048

pISSN:

2980-194X

Anahtar kelimeler:

Özet:

 Endocrine disruptors, particularly phthalates like Dimethyl phthalate DMP and Diisobutyl phthalate DiBP, are prevalent environmental contaminants posing significant health risks. This study investigates the combined neurotoxic effects of DMP and DiBP on SH-SY5Y neuroblastoma cells by analyzing cytotoxicity, oxidative stress, and apoptosis. Using MTT and Neutral Red Uptake assays, we determined the IC50 values for DMP and DiBP as 11.35 mM and 1.307 mM, respectively. Flow cytometry revealed increased Reactive Oxygen Species ROS levels, indicating oxidative stress, while apoptosis assays showed enhanced cell death with combined phthalate exposure. The results demonstrate a synergistic effect, exacerbating cytotoxic and oxidative damage beyond individual exposures. This study highlights the compounded risk of phthalate mixtures, urging comprehensive risk assessments and regulatory policies to mitigate human health risks from combined chemical exposures.

 Endocrine disruptors, particularly phthalates like Dimethyl phthalate DMP and Diisobutyl phthalate DiBP, are prevalent environmental contaminants posing significant health risks. This study investigates the combined neurotoxic effects of DMP and DiBP on SH-SY5Y neuroblastoma cells by analyzing cytotoxicity, oxidative stress, and apoptosis. Using MTT and Neutral Red Uptake assays, we determined the IC50 values for DMP and DiBP as 11.35 mM and 1.307 mM, respectively. Flow cytometry revealed increased Reactive Oxygen Species ROS levels, indicating oxidative stress, while apoptosis assays showed enhanced cell death with combined phthalate exposure. The results demonstrate a synergistic effect, exacerbating cytotoxic and oxidative damage beyond individual exposures. This study highlights the compounded risk of phthalate mixtures, urging comprehensive risk assessments and regulatory policies to mitigate human health risks from combined chemical exposures.

 Endocrine disruptors, particularly phthalates like Dimethyl phthalate DMP and Diisobutyl phthalate DiBP, are prevalent environmental contaminants posing significant health risks. This study investigates the combined neurotoxic effects of DMP and DiBP on SH-SY5Y neuroblastoma cells by analyzing cytotoxicity, oxidative stress, and apoptosis. Using MTT and Neutral Red Uptake assays, we determined the IC50 values for DMP and DiBP as 11.35 mM and 1.307 mM, respectively. Flow cytometry revealed increased Reactive Oxygen Species ROS levels, indicating oxidative stress, while apoptosis assays showed enhanced cell death with combined phthalate exposure. The results demonstrate a synergistic effect, exacerbating cytotoxic and oxidative damage beyond individual exposures. This study highlights the compounded risk of phthalate mixtures, urging comprehensive risk assessments and regulatory policies to mitigate human health risks from combined chemical exposures.

 Endocrine disruptors, particularly phthalates like Dimethyl phthalate DMP and Diisobutyl phthalate DiBP, are prevalent environmental contaminants posing significant health risks. This study investigates the combined neurotoxic effects of DMP and DiBP on SH-SY5Y neuroblastoma cells by analyzing cytotoxicity, oxidative stress, and apoptosis. Using MTT and Neutral Red Uptake assays, we determined the IC50 values for DMP and DiBP as 11.35 mM and 1.307 mM, respectively. Flow cytometry revealed increased Reactive Oxygen Species ROS levels, indicating oxidative stress, while apoptosis assays showed enhanced cell death with combined phthalate exposure. The results demonstrate a synergistic effect, exacerbating cytotoxic and oxidative damage beyond individual exposures. This study highlights the compounded risk of phthalate mixtures, urging comprehensive risk assessments and regulatory policies to mitigate human health risks from combined chemical exposures.

Makale detayları

Investigation of the Neurotoxic Effects of Dimethyl Phthalate and Diisobutyl Phthalate on SH-SY5Y Neuroblastoma Cells

Yazarlar:

Mehtap Kara | Ayşenur Erdinç | Erkan Gülgen | Yağmur Emre Arıcan | Çiğdem Sevim

Yayıncı:

Ataturk University

Ana Başlıklar:

Toxicology

Dergi:

Recent Trends in Pharmacology Vol.2 Issue 3, pp.101-108

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001337048

pISSN:

2980-194X

apoptosis

endocrine disruptors

neurotoxicity

oxidative stress

phthalates

Sayı

Makale detayları

Investigation of the Neurotoxic Effects of Dimethyl Phthalate and Diisobutyl Phthalate on SH-SY5Y Neuroblastoma Cells

Yazarlar: Mehtap Kara | Ayşenur Erdinç | Erkan Gülgen | Yağmur Emre Arıcan | Çiğdem Sevim

Yayıncı: Ataturk University

Ana Başlıklar: Toxicology

Dergi: Recent Trends in Pharmacology Vol.2 Issue 3, pp.101-108

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001337048

pISSN: 2980-194X

apoptosis

endocrine disruptors

neurotoxicity

oxidative stress

phthalates

Sayı

Yazarlar:

Mehtap Kara | Ayşenur Erdinç | Erkan Gülgen | Yağmur Emre Arıcan | Çiğdem Sevim

Yayıncı:

Ataturk University

Ana Başlıklar:

Toxicology

Dergi:

Recent Trends in Pharmacology Vol.2 Issue 3, pp.101-108

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001337048

pISSN:

2980-194X