SIRT1 Gene Polymorphisms and the Risk of Vitiligo: Molecular Association and in Silico Approach

Oktay Kuru; Nilgün Solak Tekin; Ümmühani Özel Türkcü; Sevim Karakaş Çelik; Tuba Edgünlü

Makale detayları

SIRT1 Gene Polymorphisms and the Risk of Vitiligo: Molecular Association and in Silico Approach

Yazarlar:

Oktay Kuru | Nilgün Solak Tekin | Ümmühani Özel Türkcü | Sevim Karakaş Çelik | Tuba Edgünlü

Yayıncı:

Zonguldak Bulent Ecevit University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Batı Karadeniz Tıp Dergisi Vol.7 Issue 1, pp.1-8

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001288833

pISSN:

2822-4302

Anahtar kelimeler:

Özet:

 Aim: The aim of our study is to analyze the SIRT1 gene rs2273773, rs7895833 and rs7069102 polymorphisms and the association of SIRT1 gene and interacting genes with vitiligo disease by molecular and in silico methods. Material and Methods: The study group consisted of 78 vitiligo patients and 85 unrelated healthy controls. SIRT1 polymorphisms were determined using the Polymerase chain reaction confronting twopair primers PCR-CTPP method. In addition, other genes with which the SIRT1 gene interacts and gene ontology GO were determined using the GeneMANIA and GeneCodis 4 tools, respectively. Results: We have determined a significant difference in genotypes of rs7895833 in SIRT1 gene. Especially, the AG genotype was observed more in the group with vitiligo. It was determined that the rs7895833 G allele had a protective effect in terms of vitiligo p0.001. Intergene interaction analysis was also performed by in silico method, and it was shown that SIRT 1 is co-expressed with 16 genes and shares an area with only 12 genes physically interacting with 19 genes. We showed gene ontology and pathway analyzed with all relevant genes. It was determined that especially apoptosis and systemic sclerosis were associated with these genes. Conclusion: The SIRT1 rs7895833 SNP genotype and allele frequencies of vitiligo patients are significantly different from healthy controls. Our study shows that the rs7895833 polymorphism of the SIRT1 gene may be associated with vitiligo susceptibility. Considering the role of sirtuin and related genes, especially in the apoptotic pathway, its effect on vitiligo can be further investigated to elucidate the molecular aspect of the disease.

 Aim: The aim of our study is to analyze the SIRT1 gene rs2273773, rs7895833 and rs7069102 polymorphisms and the association of SIRT1 gene and interacting genes with vitiligo disease by molecular and in silico methods. Material and Methods: The study group consisted of 78 vitiligo patients and 85 unrelated healthy controls. SIRT1 polymorphisms were determined using the Polymerase chain reaction confronting twopair primers PCR-CTPP method. In addition, other genes with which the SIRT1 gene interacts and gene ontology GO were determined using the GeneMANIA and GeneCodis 4 tools, respectively. Results: We have determined a significant difference in genotypes of rs7895833 in SIRT1 gene. Especially, the AG genotype was observed more in the group with vitiligo. It was determined that the rs7895833 G allele had a protective effect in terms of vitiligo p0.001. Intergene interaction analysis was also performed by in silico method, and it was shown that SIRT 1 is co-expressed with 16 genes and shares an area with only 12 genes physically interacting with 19 genes. We showed gene ontology and pathway analyzed with all relevant genes. It was determined that especially apoptosis and systemic sclerosis were associated with these genes. Conclusion: The SIRT1 rs7895833 SNP genotype and allele frequencies of vitiligo patients are significantly different from healthy controls. Our study shows that the rs7895833 polymorphism of the SIRT1 gene may be associated with vitiligo susceptibility. Considering the role of sirtuin and related genes, especially in the apoptotic pathway, its effect on vitiligo can be further investigated to elucidate the molecular aspect of the disease.

 Aim: The aim of our study is to analyze the SIRT1 gene rs2273773, rs7895833 and rs7069102 polymorphisms and the association of SIRT1 gene and interacting genes with vitiligo disease by molecular and in silico methods. Material and Methods: The study group consisted of 78 vitiligo patients and 85 unrelated healthy controls. SIRT1 polymorphisms were determined using the Polymerase chain reaction confronting twopair primers PCR-CTPP method. In addition, other genes with which the SIRT1 gene interacts and gene ontology GO were determined using the GeneMANIA and GeneCodis 4 tools, respectively. Results: We have determined a significant difference in genotypes of rs7895833 in SIRT1 gene. Especially, the AG genotype was observed more in the group with vitiligo. It was determined that the rs7895833 G allele had a protective effect in terms of vitiligo p0.001. Intergene interaction analysis was also performed by in silico method, and it was shown that SIRT 1 is co-expressed with 16 genes and shares an area with only 12 genes physically interacting with 19 genes. We showed gene ontology and pathway analyzed with all relevant genes. It was determined that especially apoptosis and systemic sclerosis were associated with these genes. Conclusion: The SIRT1 rs7895833 SNP genotype and allele frequencies of vitiligo patients are significantly different from healthy controls. Our study shows that the rs7895833 polymorphism of the SIRT1 gene may be associated with vitiligo susceptibility. Considering the role of sirtuin and related genes, especially in the apoptotic pathway, its effect on vitiligo can be further investigated to elucidate the molecular aspect of the disease.

 Amaç: Çalışmamızın amacı, SIRT1 geni rs2273773, rs7895833 ve rs7069102 polimorfizmlerinin ve SIRT1 geni ile etkileşimli genlerin vitiligo hastalığı ile ilişkilisinin moleküler ve in silico yöntemler ile analizini yapmaktır Gereç ve Yöntemler: Çalışma grubu 78 vitiligo hastası ve 85 sağlıklı kontrol katılımcısını kapsamaktadır. SIRT1 polimorfizmleri, iki çift primer PCR-CTPP yöntemiyle karşılıklı Polimeraz zincir reaksiyonu kullanılarak belirlendi. Ayrıca SIRT1 geninin etkileştiği diğer genler ve gen ontolojisi GO sırasıyla GeneMANIA ve GeneCodis 4 araçları kullanılarak belirlendi. Bulgular: SIRT1 geninde rs7895833 genotipinin analiz edilen gruplar arasında anlamlı bir farklılık gösterdiğini belirledik. Özellikle AG genotipi vitiligolu grupta daha fazla gözlendi. rs7895833 G allellin vitiligo açısından koruyucu bir etki gösterdiği tespit edilmiştir p0.001. In silico yöntemle genler arası etkileşim analizi de yapılarak SIRT 1in 16 gen ile birlikte eksprese edildiğini ve 19 gen ile sadece 12 gen fiziksel etkileşimi olan bir alanı paylaştığı gösterildi. İlgili tüm genlerle analiz edilen gen ontolojisini ve yolunu gösterdik. Özellikle apoptoz ve sistemik sklerozun bu genlerle ilişkili olduğunu belirlendi. Sonuç: Vitiligo hastalarının SIRT1 rs7895833 SNP genotipi ve allel frekansları, sağlıklı kontrollerden önemli ölçüde farklıdır. Çalışmamız, SIRT1 geninin rs7895833 polimorfizmiyle vitiligo duyarlılığının ilişkili olabileceğini göstermektedir. Sirtuin ve ilgili genlerin özellikle apoptotik yolaktaki görevleri göz önüne alındığında vitiligoya etkisi, hastalığın moleküler yönünü aydınlatmak için daha fazla araştırılabilir.

Makale detayları

SIRT1 Gene Polymorphisms and the Risk of Vitiligo: Molecular Association and in Silico Approach

Yazarlar:

Oktay Kuru | Nilgün Solak Tekin | Ümmühani Özel Türkcü | Sevim Karakaş Çelik | Tuba Edgünlü

Yayıncı:

Zonguldak Bulent Ecevit University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Batı Karadeniz Tıp Dergisi Vol.7 Issue 1, pp.1-8

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001288833

pISSN:

2822-4302

Gene polymorphism

In silico

SIRT1

VitiligoGen polimorfizmi

In silico

SIRT1

Vitiligo

Sayı

Makale detayları

Yazarlar: Oktay Kuru | Nilgün Solak Tekin | Ümmühani Özel Türkcü | Sevim Karakaş Çelik | Tuba Edgünlü

Yayıncı: Zonguldak Bulent Ecevit University

Ana Başlıklar: Sağlık Kurumları Yönetimi

Dergi: Batı Karadeniz Tıp Dergisi Vol.7 Issue 1, pp.1-8

Belge Tipi: Makale

Yayın Tarihi: 4/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001288833

pISSN: 2822-4302

Sayı

Yazarlar:

Oktay Kuru | Nilgün Solak Tekin | Ümmühani Özel Türkcü | Sevim Karakaş Çelik | Tuba Edgünlü

Yayıncı:

Zonguldak Bulent Ecevit University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Batı Karadeniz Tıp Dergisi Vol.7 Issue 1, pp.1-8

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001288833

pISSN:

2822-4302