Soil bacteria conferred a positive relationship and improved salt stress tolerance in transgenic pea (Pisum sativum L.) harboring Na+/H+ antiporter

Zahid ALI; Nasr ULLAH; Saadia NASEEM; Muhammad İnam Ul HAQ; Hans Joerg JACOBSEN

Makale detayları

Soil bacteria conferred a positive relationship and improved salt stress tolerance in transgenic pea (Pisum sativum L.) harboring Na+/H+ antiporter

Yazarlar:

Zahid ALI | Nasr ULLAH | Saadia NASEEM | Muhammad İnam Ul HAQ | Hans Joerg JACOBSEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2015 Vol.39 Issue 6, pp.962-972

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000815648

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Anahtar kelimeler:

Özet:

 

Among grain legumes, peas (Pisum sativum L.) are highly sensitive to salt stress. Acclimatization of plants to such conditions is mandatory. We provide improved salt stress tolerance response of transgenic pea plants overexpressing the Na+/H+ gene from Arabidopsis thaliana and a positive association with salt-tolerant plant growth-promoting rhizobacteria (PGPR). In addition to salt stress tolerance and phosphate solubilization, the selected rhizobacterial isolates were identified for indole acetic acid and proline production ability. Seed germination percentage in transgenic pea plants was significantly higher under NaCl challenge. The wild-type (WT) pea plants inoculated with known numbers of viable cells of salt-tolerant PGPR and transgenic pea plants without any inoculation showed better growth performance under salt stress. However, the PGPR-inoculated transgenic plants showed significant increase in growth and biomass compared to the WT counterpart. An increase in antioxidant enzymes, i.e. Superoxide dismutase and peroxidases, was observed in PGPR-inoculated transgenic plants under salt stress. We could not see any negative effect of the transgene in pea plants on the growth of associated PGPR. The overall impact of microbe-mediated elicitation responses in transgenic plants, whether at the biochemical or molecular level, may lead to protection against salt stress.

 

Among grain legumes, peas (Pisum sativum L.) are highly sensitive to salt stress. Acclimatization of plants to such conditions is mandatory. We provide improved salt stress tolerance response of transgenic pea plants overexpressing the Na+/H+ gene from Arabidopsis thaliana and a positive association with salt-tolerant plant growth-promoting rhizobacteria (PGPR). In addition to salt stress tolerance and phosphate solubilization, the selected rhizobacterial isolates were identified for indole acetic acid and proline production ability. Seed germination percentage in transgenic pea plants was significantly higher under NaCl challenge. The wild-type (WT) pea plants inoculated with known numbers of viable cells of salt-tolerant PGPR and transgenic pea plants without any inoculation showed better growth performance under salt stress. However, the PGPR-inoculated transgenic plants showed significant increase in growth and biomass compared to the WT counterpart. An increase in antioxidant enzymes, i.e. Superoxide dismutase and peroxidases, was observed in PGPR-inoculated transgenic plants under salt stress. We could not see any negative effect of the transgene in pea plants on the growth of associated PGPR. The overall impact of microbe-mediated elicitation responses in transgenic plants, whether at the biochemical or molecular level, may lead to protection against salt stress.

Makale detayları

Soil bacteria conferred a positive relationship and improved salt stress tolerance in transgenic pea (Pisum sativum L.) harboring Na+/H+ antiporter

Yazarlar:

Zahid ALI | Nasr ULLAH | Saadia NASEEM | Muhammad İnam Ul HAQ | Hans Joerg JACOBSEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2015 Vol.39 Issue 6, pp.962-972

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000815648

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Salt stress tolerance

transgenic plants

Na+/H+ antiporter

plant growth-promoting rhizobacteria

Pisum sativum L.

Sayı

Makale detayları

Soil bacteria conferred a positive relationship and improved salt stress tolerance in transgenic pea (Pisum sativum L.) harboring Na+/H+ antiporter

Yazarlar: Zahid ALI | Nasr ULLAH | Saadia NASEEM | Muhammad İnam Ul HAQ | Hans Joerg JACOBSEN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Botany 2015 Vol.39 Issue 6, pp.962-972

Belge Tipi: Makale

Yayın Tarihi: 12/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000815648

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Salt stress tolerance

transgenic plants

Na+/H+ antiporter

plant growth-promoting rhizobacteria

Pisum sativum L.

Sayı

Yazarlar:

Zahid ALI | Nasr ULLAH | Saadia NASEEM | Muhammad İnam Ul HAQ | Hans Joerg JACOBSEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2015 Vol.39 Issue 6, pp.962-972

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000815648

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X