Impact of melamine exposure on apoptotic proteins and oxidative stress markers in mouse hepatic tissue

Alparslan Kadir Devrim; Tuba Devrim; Hüsamettin Ekici; Mahmut Sözmen; Ali Şenol; Nilüfer Kuruca; Mert Sudağıdan

Makale detayları

Impact of melamine exposure on apoptotic proteins and oxidative stress markers in mouse hepatic tissue

Yazarlar:

Alparslan Kadir Devrim | Tuba Devrim | Hüsamettin Ekici | Mahmut Sözmen | Ali Şenol | Nilüfer Kuruca | Mert Sudağıdan

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Sciences (Other)

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.72 Issue 2, pp.211-217

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343153

pISSN:

1300-0861

Anahtar kelimeler:

Özet:

 Most melamine studies have focused on renal toxicity and its effects on the liver are still not well known. We investigated the apoptotic and oxidative effects of melamine on the liver using thirty BALBc mice, divided into three groups. The control group received saline, while the low-dose melamine LDM group was given 400 mgkg 18 LD50 and the high-dose melamine HDM group received 1600 mgkg 12 LD50 intragastrically 0.25 ml for 5 consecutive days. Liver Bcl-2 and caspase-3 expressions were analyzed at the protein level by immunohistochemistry and ELISA, and also at the gene level by quantitative Real-Time PCR. In addition, total antioxidant TAS, total oxidant TOS, and oxidative stress index OSI levels in liver tissues were measured spectrophotometrically. The immunohistochemical expression of caspase-3 was higher in the LDM and HDM groups compared to the control group p  0.002. TOS and OSI levels were increased significantly P 0.05 in the HDM group as compared to controls. Bcl-2 ELISA levels in the HDM group increased significantly compared to the control P  0.0024. Caspase-3 values increased significantly in the HDM group compared to the control P  0.0001 and LDM P  0.0016 groups. This study provides evidence that exposure to melamine induces oxidative stress and increases apoptosis in the liver. In conclusion, we suggest that both apoptotic and anti-apoptotic mechanisms may be disrupted at high melamine exposures, which has not been reported extensively in previous publications.

 Most melamine studies have focused on renal toxicity and its effects on the liver are still not well known. We investigated the apoptotic and oxidative effects of melamine on the liver using thirty BALBc mice, divided into three groups. The control group received saline, while the low-dose melamine LDM group was given 400 mgkg 18 LD50 and the high-dose melamine HDM group received 1600 mgkg 12 LD50 intragastrically 0.25 ml for 5 consecutive days. Liver Bcl-2 and caspase-3 expressions were analyzed at the protein level by immunohistochemistry and ELISA, and also at the gene level by quantitative Real-Time PCR. In addition, total antioxidant TAS, total oxidant TOS, and oxidative stress index OSI levels in liver tissues were measured spectrophotometrically. The immunohistochemical expression of caspase-3 was higher in the LDM and HDM groups compared to the control group p  0.002. TOS and OSI levels were increased significantly P 0.05 in the HDM group as compared to controls. Bcl-2 ELISA levels in the HDM group increased significantly compared to the control P  0.0024. Caspase-3 values increased significantly in the HDM group compared to the control P  0.0001 and LDM P  0.0016 groups. This study provides evidence that exposure to melamine induces oxidative stress and increases apoptosis in the liver. In conclusion, we suggest that both apoptotic and anti-apoptotic mechanisms may be disrupted at high melamine exposures, which has not been reported extensively in previous publications.

 Most melamine studies have focused on renal toxicity and its effects on the liver are still not well known. We investigated the apoptotic and oxidative effects of melamine on the liver using thirty BALBc mice, divided into three groups. The control group received saline, while the low-dose melamine LDM group was given 400 mgkg 18 LD50 and the high-dose melamine HDM group received 1600 mgkg 12 LD50 intragastrically 0.25 ml for 5 consecutive days. Liver Bcl-2 and caspase-3 expressions were analyzed at the protein level by immunohistochemistry and ELISA, and also at the gene level by quantitative Real-Time PCR. In addition, total antioxidant TAS, total oxidant TOS, and oxidative stress index OSI levels in liver tissues were measured spectrophotometrically. The immunohistochemical expression of caspase-3 was higher in the LDM and HDM groups compared to the control group p  0.002. TOS and OSI levels were increased significantly P 0.05 in the HDM group as compared to controls. Bcl-2 ELISA levels in the HDM group increased significantly compared to the control P  0.0024. Caspase-3 values increased significantly in the HDM group compared to the control P  0.0001 and LDM P  0.0016 groups. This study provides evidence that exposure to melamine induces oxidative stress and increases apoptosis in the liver. In conclusion, we suggest that both apoptotic and anti-apoptotic mechanisms may be disrupted at high melamine exposures, which has not been reported extensively in previous publications.

 Most melamine studies have focused on renal toxicity and its effects on the liver are still not well known. We investigated the apoptotic and oxidative effects of melamine on the liver using thirty BALBc mice, divided into three groups. The control group received saline, while the low-dose melamine LDM group was given 400 mgkg 18 LD50 and the high-dose melamine HDM group received 1600 mgkg 12 LD50 intragastrically 0.25 ml for 5 consecutive days. Liver Bcl-2 and caspase-3 expressions were analyzed at the protein level by immunohistochemistry and ELISA, and also at the gene level by quantitative Real-Time PCR. In addition, total antioxidant TAS, total oxidant TOS, and oxidative stress index OSI levels in liver tissues were measured spectrophotometrically. The immunohistochemical expression of caspase-3 was higher in the LDM and HDM groups compared to the control group p  0.002. TOS and OSI levels were increased significantly P 0.05 in the HDM group as compared to controls. Bcl-2 ELISA levels in the HDM group increased significantly compared to the control P  0.0024. Caspase-3 values increased significantly in the HDM group compared to the control P  0.0001 and LDM P  0.0016 groups. This study provides evidence that exposure to melamine induces oxidative stress and increases apoptosis in the liver. In conclusion, we suggest that both apoptotic and anti-apoptotic mechanisms may be disrupted at high melamine exposures, which has not been reported extensively in previous publications.

Makale detayları

Impact of melamine exposure on apoptotic proteins and oxidative stress markers in mouse hepatic tissue

Yazarlar:

Alparslan Kadir Devrim | Tuba Devrim | Hüsamettin Ekici | Mahmut Sözmen | Ali Şenol | Nilüfer Kuruca | Mert Sudağıdan

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Sciences (Other)

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.72 Issue 2, pp.211-217

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343153

pISSN:

1300-0861

Apoptosis

Bcl-2

Caspase-3

Melamine

Oxidative stress

Sayı

Makale detayları

Impact of melamine exposure on apoptotic proteins and oxidative stress markers in mouse hepatic tissue

Yazarlar: Alparslan Kadir Devrim | Tuba Devrim | Hüsamettin Ekici | Mahmut Sözmen | Ali Şenol | Nilüfer Kuruca | Mert Sudağıdan

Yayıncı: Ankara University

Ana Başlıklar: Veterinary Sciences (Other)

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.72 Issue 2, pp.211-217

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001343153

pISSN: 1300-0861

Apoptosis

Bcl-2

Caspase-3

Melamine

Oxidative stress

Sayı

Yazarlar:

Alparslan Kadir Devrim | Tuba Devrim | Hüsamettin Ekici | Mahmut Sözmen | Ali Şenol | Nilüfer Kuruca | Mert Sudağıdan

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Sciences (Other)

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.72 Issue 2, pp.211-217

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343153

pISSN:

1300-0861