Detection and molecular characterization of Macrophomina phaseolina isolates in sesame using species-specific markers

Amjad Ali; Muhammed Tatar; Fatih Ölmez; Muhammad Azhar Nadeem; Faheem Baloch

Makale detayları

Detection and molecular characterization of Macrophomina phaseolina isolates in sesame using species-specific markers

Yazarlar:

Amjad Ali | Muhammed Tatar | Fatih Ölmez | Muhammad Azhar Nadeem | Faheem Baloch

Yayıncı:

Tokat Gaziosmanpasa University

Ana Başlıklar:

Fitopatoloji

Dergi:

Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi Vol.42 Issue 2, pp.89-94

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357339

pISSN:

1300-2910

Anahtar kelimeler:

Özet:

 Sesame Sesamum indicum L. is a vital oilseed crop cultivated globally, yet its production is significantly threatened by charcoal rot disease caused by Macrophomina phaseolina. This soilborne pathogen leads to severe yield losses, affecting sesame-growing regions globally. In this study, we conducted a molecular characterization of M. phaseolina isolates collected from infected sesame plants in Diyarbakır and Şanlıurfa, Türkiye. During the field survey, the characteristic symptoms such as wilting, stunted growth, and blackened stems were observed and samples were collected to isolate the targeted pathogen. Seven isolates were successfully isolated and analyzed for their morphological and microscopic features, confirming the presence of M. phaseolina. The DNA extraction and PCR amplification using species-specific primers MpKRIMpKFI successfully detected the pathogen in all isolates, producing a distinct 350 bp amplicon. The results validate the reliability of molecular markers in identifying M. phaseolina with high specificity. This study highlights the importance of molecular tools for precise pathogen detection, aiding in disease management strategies. The findings contribute to a better understanding of M. phaseolinas genetic diversity and offer valuable insights for breeding programs aimed at developing resistant sesame cultivars. Future research should focus on integrating molecular diagnostics with breeding efforts to enhance sesames resilience against charcoal rot disease.

 Sesame Sesamum indicum L. is a vital oilseed crop cultivated globally, yet its production is significantly threatened by charcoal rot disease caused by Macrophomina phaseolina. This soilborne pathogen leads to severe yield losses, affecting sesame-growing regions globally. In this study, we conducted a molecular characterization of M. phaseolina isolates collected from infected sesame plants in Diyarbakır and Şanlıurfa, Türkiye. During the field survey, the characteristic symptoms such as wilting, stunted growth, and blackened stems were observed and samples were collected to isolate the targeted pathogen. Seven isolates were successfully isolated and analyzed for their morphological and microscopic features, confirming the presence of M. phaseolina. The DNA extraction and PCR amplification using species-specific primers MpKRIMpKFI successfully detected the pathogen in all isolates, producing a distinct 350 bp amplicon. The results validate the reliability of molecular markers in identifying M. phaseolina with high specificity. This study highlights the importance of molecular tools for precise pathogen detection, aiding in disease management strategies. The findings contribute to a better understanding of M. phaseolinas genetic diversity and offer valuable insights for breeding programs aimed at developing resistant sesame cultivars. Future research should focus on integrating molecular diagnostics with breeding efforts to enhance sesames resilience against charcoal rot disease.

 Sesame Sesamum indicum L. is a vital oilseed crop cultivated globally, yet its production is significantly threatened by charcoal rot disease caused by Macrophomina phaseolina. This soilborne pathogen leads to severe yield losses, affecting sesame-growing regions globally. In this study, we conducted a molecular characterization of M. phaseolina isolates collected from infected sesame plants in Diyarbakır and Şanlıurfa, Türkiye. During the field survey, the characteristic symptoms such as wilting, stunted growth, and blackened stems were observed and samples were collected to isolate the targeted pathogen. Seven isolates were successfully isolated and analyzed for their morphological and microscopic features, confirming the presence of M. phaseolina. The DNA extraction and PCR amplification using species-specific primers MpKRIMpKFI successfully detected the pathogen in all isolates, producing a distinct 350 bp amplicon. The results validate the reliability of molecular markers in identifying M. phaseolina with high specificity. This study highlights the importance of molecular tools for precise pathogen detection, aiding in disease management strategies. The findings contribute to a better understanding of M. phaseolinas genetic diversity and offer valuable insights for breeding programs aimed at developing resistant sesame cultivars. Future research should focus on integrating molecular diagnostics with breeding efforts to enhance sesames resilience against charcoal rot disease.

 Susam Sesamum indicum L., dünya genelinde yetiştirilen önemli bir yağ bitkisi olup, üretimi Macrophomina phaseolinanın neden olduğu kömür çürüklüğü hastalığı tarafından ciddi şekilde tehdit edilmektedir. Toprak kökenli bu patojen, susam yetiştirilen bölgelerde önemli verim kayıplarına yol açmaktadır. Bu çalışmada, Diyarbakır ve Şanlıurfa, Türkiye susam üretim alanlarındaki enfekte susam bitkilerinden izole edilen M. phaseolina izolatlarının moleküler karakterizasyonu gerçekleştirilmiştir. Arazi çalışmaları sırasında solgunluk, bodur büyüme ve siyahlaşmış gövdeler gibi karakteristik semptomlar tespit edilmiştir. Yedi izolat başarıyla elde edilerek morfolojik ve mikroskobik özellikleri incelenmiş ve M. phaseolinanın varlığı doğrulanmıştır. Türlere özgü primerler MpKRIMpKFI kullanılarak gerçekleştirilen DNA ekstraksiyonu ve PCR amplifikasyonu, tüm izolatlarda patojeni başarıyla tespit etmiş ve 350 bplik özgün bir amplikon ürünü üretmiştir. Sonuçlar, moleküler markörlerin M. phaseolinanın yüksek özgüllükle tanımlanmasındaki güvenilirliğini doğrulamaktadır. Bu çalışma, hastalık yönetim stratejilerine katkı sağlamak için moleküler araçların hassas patojen tespitindeki önemini vurgulamaktadır. Bulgular, M. phaseolinanın genetik çeşitliliğinin daha iyi anlaşılmasına katkıda bulunarak, kömür çürüklüğüne dayanıklı susam çeşitlerinin geliştirilmesine yönelik ıslah programları için değerli bilgiler sunmaktadır. Gelecek araştırmalar, susamın kömür çürüklüğüne karşı direncini artırmak amacıyla moleküler tanı yöntemleri ile ıslah çalışmalarının entegrasyonuna odaklanmalıdır.

Makale detayları

Detection and molecular characterization of Macrophomina phaseolina isolates in sesame using species-specific markers

Yazarlar:

Amjad Ali | Muhammed Tatar | Fatih Ölmez | Muhammad Azhar Nadeem | Faheem Baloch

Yayıncı:

Tokat Gaziosmanpasa University

Ana Başlıklar:

Fitopatoloji

Dergi:

Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi Vol.42 Issue 2, pp.89-94

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357339

pISSN:

1300-2910

Sesamum indicum

Macrophomina phaseolina

molecular characterization

fungal pathogens

sesame breedingSesamum indicum

Macrophomina phaseolina

moleküler karakterizasyon

fungal patojenler

Sayı

Makale detayları

Yazarlar: Amjad Ali | Muhammed Tatar | Fatih Ölmez | Muhammad Azhar Nadeem | Faheem Baloch

Yayıncı: Tokat Gaziosmanpasa University

Ana Başlıklar: Fitopatoloji

Dergi: Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi Vol.42 Issue 2, pp.89-94

Belge Tipi: Makale

Yayın Tarihi: 8/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001357339

pISSN: 1300-2910

Sayı

Yazarlar:

Amjad Ali | Muhammed Tatar | Fatih Ölmez | Muhammad Azhar Nadeem | Faheem Baloch

Yayıncı:

Tokat Gaziosmanpasa University

Ana Başlıklar:

Fitopatoloji

Dergi:

Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi Vol.42 Issue 2, pp.89-94

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357339

pISSN:

1300-2910