Synthesis, Characterization, and Use of Lanthanide Chelate of β-Diketonate Based Ligand as a Luminescent Biolabel

Semanur ŞEN YUVAYAPAN; Alper Tolga ÇOLAK; Onur ŞAHİN; Cumali CELIK

doi:https://doi.org/10.18596/jotcsa.949970

Makale detayları

Synthesis, Characterization, and Use of Lanthanide Chelate of β-Diketonate Based Ligand as a Luminescent Biolabel

Yazarlar:

Semanur ŞEN YUVAYAPAN | Alper Tolga ÇOLAK | Onur ŞAHİN | Cumali CELIK

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

İnorganik kimya

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry 2021 Vol.8 Issue 3, pp.835-850

Belge Tipi:

Makale

Yayın Tarihi:

8/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000502223

Sıklık:

Üç Aylık

eISSN:

2149-0120

DOI:

https://doi.org/10.18596/jotcsa.949970

Özet:

 


In this study, we aimed to synthesize a β-diketonate-based ligand and its Eu(III) complex which are used for luminescent biolabel. For this purpose, we chose acetophenone as the starting material which contains methyl group at the alpha position of the ketone group. Firstly, we obtained 4,4,5,5,6,6,7,7-octafluoro-3,8-dihydroxy-1,10-diphenyldeca-2,8-diene-1,10-dione (H2ODIT) ligand in a reaction between acetophenone and diethyloctafluoroadipate with Claisen condensation. This ligand was characterized by 1H-NMR, 13C-NMR, and mass spectral analyses. We obtained a single crystal H2ODIT as characterized by X-ray analysis. At the second step, in order to bind H2ODIT to the antibody, it is reacted with chlorosulfonic acid. As a result of this reaction, the functional group of -ClSO2 was bound to the structure. The structure of H2CODIT was characterized by NMR and mass spectral analysis. In the third step, to understand the usability of the ligand as a biolabel, the complex compound was synthesized with EuCl3. The complex compound was excited with UV light at 306 nm wavelength, specific hypersensitive 5D0 → 7F2 phosphorescence electronic transition of the Eu(III) was observed which proved that the luminescent H2CODIT molecule can work as a biolabel.

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Makale detayları

Synthesis, Characterization, and Use of Lanthanide Chelate of β-Diketonate Based Ligand as a Luminescent Biolabel

Yazarlar: Semanur ŞEN YUVAYAPAN | Alper Tolga ÇOLAK | Onur ŞAHİN | Cumali CELIK

Yayıncı: Turkish Chemical Society

Ana Başlıklar: İnorganik kimya

Dergi: Journal of the Turkish Chemical Society Section A: Chemistry 2021 Vol.8 Issue 3, pp.835-850

Belge Tipi: Makale

Yayın Tarihi: 8/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000502223

Sıklık: Üç Aylık

eISSN: 2149-0120

DOI: https://doi.org/10.18596/jotcsa.949970

Sayı

Yazarlar:

Semanur ŞEN YUVAYAPAN | Alper Tolga ÇOLAK | Onur ŞAHİN | Cumali CELIK

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

İnorganik kimya

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry 2021 Vol.8 Issue 3, pp.835-850

Belge Tipi:

Makale

Yayın Tarihi:

8/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000502223

Sıklık:

Üç Aylık

eISSN:

2149-0120

DOI:

https://doi.org/10.18596/jotcsa.949970