Modeling of TaqI Endonuclease Purification by HPLC

Mehmet DÜZENLİ and Kutlu Ö. ÜLGEN

Makale detayları

Modeling of TaqI Endonuclease Purification by HPLC

Yazarlar:

Mehmet DÜZENLİ and Kutlu Ö. ÜLGEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2002 Vol.26 Issue 6, pp.877-888

Belge Tipi:

Makale

Yayın Tarihi:

5/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000729723

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

The present work is based on the previously reported purification  study of TaqI endonuclease5 and aimed to simulate the  experimentally obtained chromatograms in order to reduce the time and  cost in the design, optimization and scaling up of purification processes.   Three different models depending on equilibrium, rate and plate  theories are used in the mathematical description of experimental elution  profiles and the solutions of the model equations are compared.  The  equilibrium-dispersive theory provides excellent results in predicting  the experimental chromatograms.  It is thus recognized that band  dispersion takes place in the column through axial dispersion and  nonequilibrium effects.  The user-friendly software written in Visual  Basic can be used in the design of purification processes as well as in  the determination of optimal separation conditions for many other  enzymes.

 

The present work is based on the previously reported purification  study of TaqI endonuclease5 and aimed to simulate the  experimentally obtained chromatograms in order to reduce the time and  cost in the design, optimization and scaling up of purification processes.   Three different models depending on equilibrium, rate and plate  theories are used in the mathematical description of experimental elution  profiles and the solutions of the model equations are compared.  The  equilibrium-dispersive theory provides excellent results in predicting  the experimental chromatograms.  It is thus recognized that band  dispersion takes place in the column through axial dispersion and  nonequilibrium effects.  The user-friendly software written in Visual  Basic can be used in the design of purification processes as well as in  the determination of optimal separation conditions for many other  enzymes.

Makale detayları

Modeling of TaqI Endonuclease Purification by HPLC

Yazarlar: Mehmet DÜZENLİ and Kutlu Ö. ÜLGEN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2002 Vol.26 Issue 6, pp.877-888

Belge Tipi: Makale

Yayın Tarihi: 5/2002

Belge Dili: Türkçe

Document ID (Org No.): 2000729723

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

Mehmet DÜZENLİ and Kutlu Ö. ÜLGEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2002 Vol.26 Issue 6, pp.877-888

Belge Tipi:

Makale

Yayın Tarihi:

5/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000729723

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527