Effect of Ricinus Communis on the Viability of Human Gingival Fibroblast Cells: An In Vitro Study

Zeynep Ezgi Akan; Sema Tuğçe Aydın; Ayşegül Tiryaki; Dilruba Baykara; Canan Ekinci Doğan; Oğuzhan Gündüz; Turgut Taşkın; Umut Ulaş Tosun; Ömer Birkan Ağralı

Makale detayları

Effect of Ricinus Communis on the Viability of Human Gingival Fibroblast Cells: An In Vitro Study

Yazarlar:

Zeynep Ezgi Akan | Sema Tuğçe Aydın | Ayşegül Tiryaki | Dilruba Baykara | Canan Ekinci Doğan | Oğuzhan Gündüz | Turgut Taşkın | Umut Ulaş Tosun | Ömer Birkan Ağralı

Yayıncı:

Marmara University

Ana Başlıklar:

Periodontics

Dergi:

European Journal of Research in Dentistry Vol.9 Issue 1, pp.33-38

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001341435

pISSN:

2630-6247

Anahtar kelimeler:

Özet:

 Objective: The aim of this study is to evaluate the potential effects of Ricinus communis extract on cell viability in human gingival fibroblast cells iDEF. Materials and Methods: Ricinus communis seeds were used to prepare the extract using ultrasonic extraction and methanol solvent. The prepared extract was diluted to concentrations of 5 µgml, 10 µgml, and 15 µgml. iDEF cells were treated with Ricinus communis extracts for 24 hours. Cell viability was assessed using the 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide MTT assay. The data were analyzed statistically using the SPSS software, and statistical significance was set at p0.05. Results: No statistically significant differences were observed between the Ricinus communis treated groups and the control group at all concentrations. Conclusion: These findings suggest that Ricinus communis extract did not have harmful effects on iDEF cells, but did not show a significant positive effect on cell viability. Therefore, further research is required to comprehensively assess the potential effects of Ricinus communis extract on wound healing.

 Objective: The aim of this study is to evaluate the potential effects of Ricinus communis extract on cell viability in human gingival fibroblast cells iDEF. Materials and Methods: Ricinus communis seeds were used to prepare the extract using ultrasonic extraction and methanol solvent. The prepared extract was diluted to concentrations of 5 µgml, 10 µgml, and 15 µgml. iDEF cells were treated with Ricinus communis extracts for 24 hours. Cell viability was assessed using the 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide MTT assay. The data were analyzed statistically using the SPSS software, and statistical significance was set at p0.05. Results: No statistically significant differences were observed between the Ricinus communis treated groups and the control group at all concentrations. Conclusion: These findings suggest that Ricinus communis extract did not have harmful effects on iDEF cells, but did not show a significant positive effect on cell viability. Therefore, further research is required to comprehensively assess the potential effects of Ricinus communis extract on wound healing.

 Objective: The aim of this study is to evaluate the potential effects of Ricinus communis extract on cell viability in human gingival fibroblast cells iDEF. Materials and Methods: Ricinus communis seeds were used to prepare the extract using ultrasonic extraction and methanol solvent. The prepared extract was diluted to concentrations of 5 µgml, 10 µgml, and 15 µgml. iDEF cells were treated with Ricinus communis extracts for 24 hours. Cell viability was assessed using the 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide MTT assay. The data were analyzed statistically using the SPSS software, and statistical significance was set at p0.05. Results: No statistically significant differences were observed between the Ricinus communis treated groups and the control group at all concentrations. Conclusion: These findings suggest that Ricinus communis extract did not have harmful effects on iDEF cells, but did not show a significant positive effect on cell viability. Therefore, further research is required to comprehensively assess the potential effects of Ricinus communis extract on wound healing.

 Amaç: Bu çalışmanın amacı, Ricinus communis ekstresinin insan diş eti fibroblastlarında iDEF hücre canlılığı üzerindeki potansiyel etkilerini değerlendirmektir. Gereç ve Yöntemler: Ricinus communis tohumlarından ultrasonik yöntem ve metanol çözücüsü kullanılarak ekstre elde edilmiştir. Hazırlanan ekstre 5 µgml, 10 µgml ve 15 µgml olacak şekilde üç farklı konsantrasyonlara seyreltildi. iDEF hücrelerine 24 saat süreyle Ricinus communis ekstreleri uygulandı. Canlılık değerlendirmesi için 3-4,5-dimetiltiyazol-2-il-2-5-difeniltetrazolyum bromür MTT analizi uygulandı. Verilerin istatistiksel analizleri SPSS paket programı kullanılarak değerlendirildi. İstatistiksel anlamlılık p0.05 olarak kabul edildi. Bulgular: Ricinus communisin uygulandığı tüm konsantrasyonlarda kontrol grubuna kıyasla istatistiksel olarak anlamlı fark tespit edilmedi. Sonuç: Bu bulgular, Ricinus communis ekstresinin iDEF hücrelerinde zararlı etkiler yaratmadığını, ancak hücre canlılığı üzerinde belirgin bir olumlu etki potansiyeline sahip olmadığını göstermektedir. Dolayısıyla, Ricinus communis ekstresinin yara iyileşmesi üzerindeki potansiyel etkilerini daha kapsamlı şekilde değerlendirebilmek için ek araştırmalar gerekmektedir.

Makale detayları

Effect of Ricinus Communis on the Viability of Human Gingival Fibroblast Cells: An In Vitro Study

Yazarlar:

Zeynep Ezgi Akan | Sema Tuğçe Aydın | Ayşegül Tiryaki | Dilruba Baykara | Canan Ekinci Doğan | Oğuzhan Gündüz | Turgut Taşkın | Umut Ulaş Tosun | Ömer Birkan Ağralı

Yayıncı:

Marmara University

Ana Başlıklar:

Periodontics

Dergi:

European Journal of Research in Dentistry Vol.9 Issue 1, pp.33-38

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001341435

pISSN:

2630-6247

Ricinus communis

fibroblasts

cell viabilityRicinus communis

fibroblastlar

hücre canlılığı

Sayı

Makale detayları

Effect of Ricinus Communis on the Viability of Human Gingival Fibroblast Cells: An In Vitro Study

Yazarlar: Zeynep Ezgi Akan | Sema Tuğçe Aydın | Ayşegül Tiryaki | Dilruba Baykara | Canan Ekinci Doğan | Oğuzhan Gündüz | Turgut Taşkın | Umut Ulaş Tosun | Ömer Birkan Ağralı

Yayıncı: Marmara University

Ana Başlıklar: Periodontics

Dergi: European Journal of Research in Dentistry Vol.9 Issue 1, pp.33-38

Belge Tipi: Makale

Yayın Tarihi: 4/2025

Belge Dili: Türkçe

Document ID (Org No.): 2001341435

pISSN: 2630-6247

Ricinus communis

fibroblasts

cell viabilityRicinus communis

fibroblastlar

hücre canlılığı

Sayı

Yazarlar:

Zeynep Ezgi Akan | Sema Tuğçe Aydın | Ayşegül Tiryaki | Dilruba Baykara | Canan Ekinci Doğan | Oğuzhan Gündüz | Turgut Taşkın | Umut Ulaş Tosun | Ömer Birkan Ağralı

Yayıncı:

Marmara University

Ana Başlıklar:

Periodontics

Dergi:

European Journal of Research in Dentistry Vol.9 Issue 1, pp.33-38

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001341435

pISSN:

2630-6247