The 3'UTR 188 C>T Polymorphism of the OLR1 Gene: Effects in Serum Oxidized LDL Levels and Metabolic Parameters in Patients with Coronary Artery Disease

Fidan Malikova; Hülya Yılmaz Aydoğan; Oğuz Öztürk; Zehra Buğra; Özlem Kurnaz Gömleksiz

Makale detayları

The 3'UTR 188 C>T Polymorphism of the OLR1 Gene: Effects in Serum Oxidized LDL Levels and Metabolic Parameters in Patients with Coronary Artery Disease

Yazarlar:

Fidan Malikova | Hülya Yılmaz Aydoğan | Oğuz Öztürk | Zehra Buğra | Özlem Kurnaz Gömleksiz

Yayıncı:

İstanbul Gelisim University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

İstanbul Gelişim Üniversitesi Sağlık Bilimleri Dergisi Issue 19, pp.82-97

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001288269

pISSN:

2536-4499

Anahtar kelimeler:

Özet:

 Aim: Oxidized LDL, which stimulates signaling pathways that activate inflammatory reactions in endothelial cells during atherosclerosis, is defined as an important pathogenic factor in the development of cardiovascular diseases. The oxidized LDL receptor LOX-1, which is the main receptor for oxidized LDL in endothelial cells, has the ability to specifically bind, internalize and degrade oxidized LDLs in endothelial cells. In this study, it was aimed to investigate the effect of 3UTR188CT polymorphism in the OLR1 gene encoding LOX-1 on serum oxidized LDL and lipid levels in the study groups consisting of coronary artery disease CAD patients and healthy controls, and to determine its relationship with lipid parameters in patients with CAD. Methods: Polymerase Chain Reaction and Restriction Fragment Length Polymorphism methods were used to detect the OLR1 gene 3UTR188CT polymorphism in groups of 50 CAD patients and 34 healthy controls. Statistical analyzes were performed with SPSS 20.0. Results: In the CAD group, oxidized LDL p0.001, total-cholesterol p0.020, Body Mass Indeks BMI p0.015, systolic p0.001 and diastolic p0.002 blood pressure levels and smoking frequency p0.002 were higher, and serum HDL-C levels p0.001 were lower compared to healthy controls. OLR1 3UTR188CT genotype and allele distributions were found to be similar between the groups p0.05. The 188T allele was associated with higher oxidized LDL levels in both CAD p0.001 and control p0.013 groups. Patients with the 188TT genotype had high oxidized LDL p0.001, total cholesterol, LDL-cholesterol, triglyceride, and VLDL-cholesterol levels. Similarly, in healthy controls, the 188TT genotype was associated with high oxidized LDL, total- and LDL-cholesterol, triglyceride, VLDL-cholesterol, systolic blood pressure, and low serum HDL-cholesterol. Conclusion: Our findings indicate that the OLR1 3UTR188CT polymorphism may be associated with the development of CAD with its effects in favor of both serum oxidized LDL, which is a proatherogenic molecule, and an atherogenic lipid profile.

 Aim: Oxidized LDL, which stimulates signaling pathways that activate inflammatory reactions in endothelial cells during atherosclerosis, is defined as an important pathogenic factor in the development of cardiovascular diseases. The oxidized LDL receptor LOX-1, which is the main receptor for oxidized LDL in endothelial cells, has the ability to specifically bind, internalize and degrade oxidized LDLs in endothelial cells. In this study, it was aimed to investigate the effect of 3UTR188CT polymorphism in the OLR1 gene encoding LOX-1 on serum oxidized LDL and lipid levels in the study groups consisting of coronary artery disease CAD patients and healthy controls, and to determine its relationship with lipid parameters in patients with CAD. Methods: Polymerase Chain Reaction and Restriction Fragment Length Polymorphism methods were used to detect the OLR1 gene 3UTR188CT polymorphism in groups of 50 CAD patients and 34 healthy controls. Statistical analyzes were performed with SPSS 20.0. Results: In the CAD group, oxidized LDL p0.001, total-cholesterol p0.020, Body Mass Indeks BMI p0.015, systolic p0.001 and diastolic p0.002 blood pressure levels and smoking frequency p0.002 were higher, and serum HDL-C levels p0.001 were lower compared to healthy controls. OLR1 3UTR188CT genotype and allele distributions were found to be similar between the groups p0.05. The 188T allele was associated with higher oxidized LDL levels in both CAD p0.001 and control p0.013 groups. Patients with the 188TT genotype had high oxidized LDL p0.001, total cholesterol, LDL-cholesterol, triglyceride, and VLDL-cholesterol levels. Similarly, in healthy controls, the 188TT genotype was associated with high oxidized LDL, total- and LDL-cholesterol, triglyceride, VLDL-cholesterol, systolic blood pressure, and low serum HDL-cholesterol. Conclusion: Our findings indicate that the OLR1 3UTR188CT polymorphism may be associated with the development of CAD with its effects in favor of both serum oxidized LDL, which is a proatherogenic molecule, and an atherogenic lipid profile.

 Aim: Oxidized LDL, which stimulates signaling pathways that activate inflammatory reactions in endothelial cells during atherosclerosis, is defined as an important pathogenic factor in the development of cardiovascular diseases. The oxidized LDL receptor LOX-1, which is the main receptor for oxidized LDL in endothelial cells, has the ability to specifically bind, internalize and degrade oxidized LDLs in endothelial cells. In this study, it was aimed to investigate the effect of 3UTR188CT polymorphism in the OLR1 gene encoding LOX-1 on serum oxidized LDL and lipid levels in the study groups consisting of coronary artery disease CAD patients and healthy controls, and to determine its relationship with lipid parameters in patients with CAD. Methods: Polymerase Chain Reaction and Restriction Fragment Length Polymorphism methods were used to detect the OLR1 gene 3UTR188CT polymorphism in groups of 50 CAD patients and 34 healthy controls. Statistical analyzes were performed with SPSS 20.0. Results: In the CAD group, oxidized LDL p0.001, total-cholesterol p0.020, Body Mass Indeks BMI p0.015, systolic p0.001 and diastolic p0.002 blood pressure levels and smoking frequency p0.002 were higher, and serum HDL-C levels p0.001 were lower compared to healthy controls. OLR1 3UTR188CT genotype and allele distributions were found to be similar between the groups p0.05. The 188T allele was associated with higher oxidized LDL levels in both CAD p0.001 and control p0.013 groups. Patients with the 188TT genotype had high oxidized LDL p0.001, total cholesterol, LDL-cholesterol, triglyceride, and VLDL-cholesterol levels. Similarly, in healthy controls, the 188TT genotype was associated with high oxidized LDL, total- and LDL-cholesterol, triglyceride, VLDL-cholesterol, systolic blood pressure, and low serum HDL-cholesterol. Conclusion: Our findings indicate that the OLR1 3UTR188CT polymorphism may be associated with the development of CAD with its effects in favor of both serum oxidized LDL, which is a proatherogenic molecule, and an atherogenic lipid profile.

 Amaç: Ateroskleroz sürecinde endotelyal hücrelerde enflamatuvar tepkimeleri aktive eden sinyal yolaklarını uyaran okside LDL, kardiyovasküler hastalıkların gelişiminde önemli bir patojenik faktör olarak tanımlanmaktadır. Endotel hücrelerinde okside LDL için başlıca reseptör olan okside LDL reseptörü LOX-1, okside LDLleri endotel hücrelerinde özgül olarak bağlayabilme, hücre içine alabilme ve degrade edebilme özelliğine sahiptir. Bu çalışmada LOX-1i kodlayan OLR1 geninde 3UTR188CT polimorfizminin koroner arter hastaları ve sağlıklı kontrollerden oluşan çalışma gruplarında serum okside LDL ve lipid düzeylerine etkisinin araştırılarak KAH hastalarında lipid parametrelerle ilişkisinin belirlenmesi amaçlanmıştır. Yöntem: Çalışmaya katılan 50 KAH hastası ve 34 sağlıklı kontrolden oluşan gruplarda OLR1 geni 3UTR188CT polimorfizminin tespiti için Polimeraz Zincir Reaksiyonu ve Restriksiyon Fragman Uzunluk Polimorfizmi yöntemleri kullanılmıştır. İstatistiksel analiz SPSS 20.0 ile yapılmıştır. Bulgular: KAH grubunda okside LDL p0,001, total-kolesterol p0,020, Beden Kütle İndeksi BKİ p0,015, sistolik p0,001 ve diastolik p0,002 kan basıncı değerleri ve sigara kullanım sıklığı p0,002 sağlıklı kontrollere kıyasla yüksek ve serum HDL-K düzeyi düşük gözlenmiştir p0,001. OLR1 3UTR188CT genotip ve allel dağılımları gruplar arasında benzer bulunmuştur p0,05. 188T alleli hem KAH p0,001 hem de kontrol p0,013 gruplarında yüksek okside LDL düzeyleri ile ilişkili gözlenmiştir. 188TT genotipi taşıyan hastalarda okside LDL p0,001, total-kolesterol, LDL-kolesterol, trigliserid ve VLDL-kolesterol düzeyleri yüksekti. Benzer şekilde sağlıklı kontrollerde de 188TT genotipi yüksek okside LDL, total ve LDL-kolesterol, trigliserid, VLDL-kolesterol, sistolik kan basıncı ve düşük serum HDL-kolesterol düzeyi ile ilişkiliydi. Sonuç: Bulgular OLR1 3UTR 188 CT polimorfizminin hem proaterojenik bir molekül olan serum okside LDL hem de aterojenik lipid profili lehine etkileriyle KAH gelişimiyle ilişkili olabileceğine işaret etmektedir.

Makale detayları

The 3'UTR 188 C>T Polymorphism of the OLR1 Gene: Effects in Serum Oxidized LDL Levels and Metabolic Parameters in Patients with Coronary Artery Disease

Yazarlar:

Fidan Malikova | Hülya Yılmaz Aydoğan | Oğuz Öztürk | Zehra Buğra | Özlem Kurnaz Gömleksiz

Yayıncı:

İstanbul Gelisim University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

İstanbul Gelişim Üniversitesi Sağlık Bilimleri Dergisi Issue 19, pp.82-97

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001288269

pISSN:

2536-4499

Oxidized LDL

LOX-1

OLR1

gene

lipid

coronary artery diseaseOkside LDL

LOX-1

OLR1

gen

lipid

koroner arter hastalığı

Sayı

Makale detayları

Yazarlar: Fidan Malikova | Hülya Yılmaz Aydoğan | Oğuz Öztürk | Zehra Buğra | Özlem Kurnaz Gömleksiz

Yayıncı: İstanbul Gelisim University

Ana Başlıklar: Klinik Tıp Bilimleri

Dergi: İstanbul Gelişim Üniversitesi Sağlık Bilimleri Dergisi Issue 19, pp.82-97

Belge Tipi: Makale

Yayın Tarihi: 4/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001288269

pISSN: 2536-4499

Sayı

Yazarlar:

Fidan Malikova | Hülya Yılmaz Aydoğan | Oğuz Öztürk | Zehra Buğra | Özlem Kurnaz Gömleksiz

Yayıncı:

İstanbul Gelisim University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

İstanbul Gelişim Üniversitesi Sağlık Bilimleri Dergisi Issue 19, pp.82-97

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001288269

pISSN:

2536-4499