is HPV16 E7 Oncoprotein Labile if it is Unliganded?

Özlem Cesur Günay; Nicola Stonehouse

Makale detayları

is HPV16 E7 Oncoprotein Labile if it is Unliganded?

Yazarlar:

Özlem Cesur Günay | Nicola Stonehouse

Yayıncı:

Marmara University

Ana Başlıklar:

Molecular Targets | Medical Biochemistry - Proteins | Peptides and Proteomics | Gene and Molecular Therapy

Dergi:

Clinical and Experimental Health Sciences Vol.15 Issue 3, pp.489-494

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359593

pISSN:

2459-1459

Anahtar kelimeler:

Özet:

 Objective: Human papillomavirus HPV infection is a major global health concern due to its association with several types of cancer, particularly cervical cancer. Among the various viral proteins produced by HPV, E6 and E7 oncoproteins have been identified as key players in the oncogenic transformation of infected cells. HPV E7 oncoprotein primarily targets the retinoblastoma protein pRb and related family members, retinoblastoma-like protein 2 p130 and retinoblastoma-like protein 1 p107 and disrupts their association with E2F transcription factors, releasing the E2F for transcriptional activation of genes required for cell cycle progression. E7 is an unstable protein with a half-life of less than 2 hours. We aimed to investigate whether the stability of the E7 protein would change when it has a binding partner such as pRb, p130 and p107. Methods: We transfected HEK293T cells with either Green Fluorescent Protein tagged E7 GFP-E7 construct alone or together with one of the pRb RB1, p130 RBL2 or p107 RBL1 constructs and observed the expression of GFP-E7 under a fluorescent microscope. We have also analysed the expression levels of E7, pRb, p130 and p107 by western blotting using specific antibodies. Results: We showed that E7 expression was more prominent in human embryonic kidney 293T HEK293T cells where one of the cellular target proteins was co-expressed. Conclusion: HPV E7 oncoprotein is an indispensable component for the pathogenesis of cervical cancer, and understanding the molecular mechanisms of how E7 maintains its stability in the cell may enable the development of targeted treatment approaches.

 Objective: Human papillomavirus HPV infection is a major global health concern due to its association with several types of cancer, particularly cervical cancer. Among the various viral proteins produced by HPV, E6 and E7 oncoproteins have been identified as key players in the oncogenic transformation of infected cells. HPV E7 oncoprotein primarily targets the retinoblastoma protein pRb and related family members, retinoblastoma-like protein 2 p130 and retinoblastoma-like protein 1 p107 and disrupts their association with E2F transcription factors, releasing the E2F for transcriptional activation of genes required for cell cycle progression. E7 is an unstable protein with a half-life of less than 2 hours. We aimed to investigate whether the stability of the E7 protein would change when it has a binding partner such as pRb, p130 and p107. Methods: We transfected HEK293T cells with either Green Fluorescent Protein tagged E7 GFP-E7 construct alone or together with one of the pRb RB1, p130 RBL2 or p107 RBL1 constructs and observed the expression of GFP-E7 under a fluorescent microscope. We have also analysed the expression levels of E7, pRb, p130 and p107 by western blotting using specific antibodies. Results: We showed that E7 expression was more prominent in human embryonic kidney 293T HEK293T cells where one of the cellular target proteins was co-expressed. Conclusion: HPV E7 oncoprotein is an indispensable component for the pathogenesis of cervical cancer, and understanding the molecular mechanisms of how E7 maintains its stability in the cell may enable the development of targeted treatment approaches.

 Objective: Human papillomavirus HPV infection is a major global health concern due to its association with several types of cancer, particularly cervical cancer. Among the various viral proteins produced by HPV, E6 and E7 oncoproteins have been identified as key players in the oncogenic transformation of infected cells. HPV E7 oncoprotein primarily targets the retinoblastoma protein pRb and related family members, retinoblastoma-like protein 2 p130 and retinoblastoma-like protein 1 p107 and disrupts their association with E2F transcription factors, releasing the E2F for transcriptional activation of genes required for cell cycle progression. E7 is an unstable protein with a half-life of less than 2 hours. We aimed to investigate whether the stability of the E7 protein would change when it has a binding partner such as pRb, p130 and p107. Methods: We transfected HEK293T cells with either Green Fluorescent Protein tagged E7 GFP-E7 construct alone or together with one of the pRb RB1, p130 RBL2 or p107 RBL1 constructs and observed the expression of GFP-E7 under a fluorescent microscope. We have also analysed the expression levels of E7, pRb, p130 and p107 by western blotting using specific antibodies. Results: We showed that E7 expression was more prominent in human embryonic kidney 293T HEK293T cells where one of the cellular target proteins was co-expressed. Conclusion: HPV E7 oncoprotein is an indispensable component for the pathogenesis of cervical cancer, and understanding the molecular mechanisms of how E7 maintains its stability in the cell may enable the development of targeted treatment approaches.

 Objective: Human papillomavirus HPV infection is a major global health concern due to its association with several types of cancer, particularly cervical cancer. Among the various viral proteins produced by HPV, E6 and E7 oncoproteins have been identified as key players in the oncogenic transformation of infected cells. HPV E7 oncoprotein primarily targets the retinoblastoma protein pRb and related family members, retinoblastoma-like protein 2 p130 and retinoblastoma-like protein 1 p107 and disrupts their association with E2F transcription factors, releasing the E2F for transcriptional activation of genes required for cell cycle progression. E7 is an unstable protein with a half-life of less than 2 hours. We aimed to investigate whether the stability of the E7 protein would change when it has a binding partner such as pRb, p130 and p107. Methods: We transfected HEK293T cells with either Green Fluorescent Protein tagged E7 GFP-E7 construct alone or together with one of the pRb RB1, p130 RBL2 or p107 RBL1 constructs and observed the expression of GFP-E7 under a fluorescent microscope. We have also analysed the expression levels of E7, pRb, p130 and p107 by western blotting using specific antibodies. Results: We showed that E7 expression was more prominent in human embryonic kidney 293T HEK293T cells where one of the cellular target proteins was co-expressed. Conclusion: HPV E7 oncoprotein is an indispensable component for the pathogenesis of cervical cancer, and understanding the molecular mechanisms of how E7 maintains its stability in the cell may enable the development of targeted treatment approaches.

Makale detayları

is HPV16 E7 Oncoprotein Labile if it is Unliganded?

Yazarlar:

Özlem Cesur Günay | Nicola Stonehouse

Yayıncı:

Marmara University

Ana Başlıklar:

Molecular Targets | Medical Biochemistry - Proteins | Peptides and Proteomics | Gene and Molecular Therapy

Dergi:

Clinical and Experimental Health Sciences Vol.15 Issue 3, pp.489-494

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359593

pISSN:

2459-1459

Human papilloma viruses

Uterine cervical neoplasms

Protein stability

Papillomavirus E7 proteins

Green fluorescent proteins

Sayı

Makale detayları

is HPV16 E7 Oncoprotein Labile if it is Unliganded?

Yazarlar: Özlem Cesur Günay | Nicola Stonehouse

Yayıncı: Marmara University

Ana Başlıklar: Molecular Targets | Medical Biochemistry - Proteins | Peptides and Proteomics | Gene and Molecular Therapy

Dergi: Clinical and Experimental Health Sciences Vol.15 Issue 3, pp.489-494

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001359593

pISSN: 2459-1459

Human papilloma viruses

Uterine cervical neoplasms

Protein stability

Papillomavirus E7 proteins

Green fluorescent proteins

Sayı

Yazarlar:

Özlem Cesur Günay | Nicola Stonehouse

Yayıncı:

Marmara University

Ana Başlıklar:

Molecular Targets | Medical Biochemistry - Proteins | Peptides and Proteomics | Gene and Molecular Therapy

Dergi:

Clinical and Experimental Health Sciences Vol.15 Issue 3, pp.489-494

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359593

pISSN:

2459-1459