In Silico Analyzing 37 kDa Salivary Protein D7 and Discovering Vaccine Candidate and Diagnostic Epitopes to Develop a Transmission-blocking Multi-epitope Vector Vaccine Against Aedes Species (A. aegypti and A. albopictus) Transmitting Several Arboviral Pathogens

Ahmet Köseoğlu; Buminhan Özgültekin; Yusuf Şeflekçi

Makale detayları

In Silico Analyzing 37 kDa Salivary Protein D7 and Discovering Vaccine Candidate and Diagnostic Epitopes to Develop a Transmission-blocking Multi-epitope Vector Vaccine Against Aedes Species (A. aegypti and A. albopictus) Transmitting Several Arboviral Pathogens

Yazarlar:

Ahmet Köseoğlu | Buminhan Özgültekin | Yusuf Şeflekçi

Yayıncı:

Tokat Gaziosmanpasa University

Ana Başlıklar:

Bioinformatics and Computational Biology (Other) | Entomology | Parasitology

Dergi:

Journal of New Results in Science Vol.14 Issue 2, pp.152-165

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357695

pISSN:

1304-7981

Anahtar kelimeler:

Özet:

 Aedes mosquitoes, primarily Aedes aegypti and Aedes albopictus, are significant vectors of arboviral diseases, including dengue, chikungunya, Zika, and yellow fever. With expanding geographical distributions due to climate change and urbanization, these species increasingly threaten public health. Current vector control strategies rely heavily on insecticides, which face challenges due to increased resistance. An alternative approach involves developing transmission-blocking vaccines targeting mosquito vector proteins rather than individual pathogens. This study performed an in-silico analysis of the 37 kDa salivary protein D7, a highly conserved and abundantly secreted protein in Aedes saliva, to identify potential vaccine and diagnostic epitopes. Through sequence comparison, structural modeling, and immunoinformatics approaches, B-cell, MHC-I, and MHC-II epitopes with high antigenicity, non-allergenicity, and non-toxicity were predicted. Selected epitopes were incorporated into multi-epitope vaccine constructs against A. aegypti and A. albopictus vectors, which were subsequently modeled, validated, and simulated to induce IgM, IgG, and IFN-g. The present studys findings suggest that the 37 kDa salivary protein D7 is a promising candidate for a transmission-blocking vector vaccine targeting Aedes mosquitoes, offering a novel strategy for controlling mosquito-borne diseases. Further experimental validation is necessary to confirm the immunogenicity and protective potential of the identified epitopes.

 Aedes mosquitoes, primarily Aedes aegypti and Aedes albopictus, are significant vectors of arboviral diseases, including dengue, chikungunya, Zika, and yellow fever. With expanding geographical distributions due to climate change and urbanization, these species increasingly threaten public health. Current vector control strategies rely heavily on insecticides, which face challenges due to increased resistance. An alternative approach involves developing transmission-blocking vaccines targeting mosquito vector proteins rather than individual pathogens. This study performed an in-silico analysis of the 37 kDa salivary protein D7, a highly conserved and abundantly secreted protein in Aedes saliva, to identify potential vaccine and diagnostic epitopes. Through sequence comparison, structural modeling, and immunoinformatics approaches, B-cell, MHC-I, and MHC-II epitopes with high antigenicity, non-allergenicity, and non-toxicity were predicted. Selected epitopes were incorporated into multi-epitope vaccine constructs against A. aegypti and A. albopictus vectors, which were subsequently modeled, validated, and simulated to induce IgM, IgG, and IFN-g. The present studys findings suggest that the 37 kDa salivary protein D7 is a promising candidate for a transmission-blocking vector vaccine targeting Aedes mosquitoes, offering a novel strategy for controlling mosquito-borne diseases. Further experimental validation is necessary to confirm the immunogenicity and protective potential of the identified epitopes.

 Aedes mosquitoes, primarily Aedes aegypti and Aedes albopictus, are significant vectors of arboviral diseases, including dengue, chikungunya, Zika, and yellow fever. With expanding geographical distributions due to climate change and urbanization, these species increasingly threaten public health. Current vector control strategies rely heavily on insecticides, which face challenges due to increased resistance. An alternative approach involves developing transmission-blocking vaccines targeting mosquito vector proteins rather than individual pathogens. This study performed an in-silico analysis of the 37 kDa salivary protein D7, a highly conserved and abundantly secreted protein in Aedes saliva, to identify potential vaccine and diagnostic epitopes. Through sequence comparison, structural modeling, and immunoinformatics approaches, B-cell, MHC-I, and MHC-II epitopes with high antigenicity, non-allergenicity, and non-toxicity were predicted. Selected epitopes were incorporated into multi-epitope vaccine constructs against A. aegypti and A. albopictus vectors, which were subsequently modeled, validated, and simulated to induce IgM, IgG, and IFN-g. The present studys findings suggest that the 37 kDa salivary protein D7 is a promising candidate for a transmission-blocking vector vaccine targeting Aedes mosquitoes, offering a novel strategy for controlling mosquito-borne diseases. Further experimental validation is necessary to confirm the immunogenicity and protective potential of the identified epitopes.

 Aedes mosquitoes, primarily Aedes aegypti and Aedes albopictus, are significant vectors of arboviral diseases, including dengue, chikungunya, Zika, and yellow fever. With expanding geographical distributions due to climate change and urbanization, these species increasingly threaten public health. Current vector control strategies rely heavily on insecticides, which face challenges due to increased resistance. An alternative approach involves developing transmission-blocking vaccines targeting mosquito vector proteins rather than individual pathogens. This study performed an in-silico analysis of the 37 kDa salivary protein D7, a highly conserved and abundantly secreted protein in Aedes saliva, to identify potential vaccine and diagnostic epitopes. Through sequence comparison, structural modeling, and immunoinformatics approaches, B-cell, MHC-I, and MHC-II epitopes with high antigenicity, non-allergenicity, and non-toxicity were predicted. Selected epitopes were incorporated into multi-epitope vaccine constructs against A. aegypti and A. albopictus vectors, which were subsequently modeled, validated, and simulated to induce IgM, IgG, and IFN-g. The present studys findings suggest that the 37 kDa salivary protein D7 is a promising candidate for a transmission-blocking vector vaccine targeting Aedes mosquitoes, offering a novel strategy for controlling mosquito-borne diseases. Further experimental validation is necessary to confirm the immunogenicity and protective potential of the identified epitopes.

Makale detayları

In Silico Analyzing 37 kDa Salivary Protein D7 and Discovering Vaccine Candidate and Diagnostic Epitopes to Develop a Transmission-blocking Multi-epitope Vector Vaccine Against Aedes Species (A. aegypti and A. albopictus) Transmitting Several Arboviral Pathogens

Yazarlar:

Ahmet Köseoğlu | Buminhan Özgültekin | Yusuf Şeflekçi

Yayıncı:

Tokat Gaziosmanpasa University

Ana Başlıklar:

Bioinformatics and Computational Biology (Other) | Entomology | Parasitology

Dergi:

Journal of New Results in Science Vol.14 Issue 2, pp.152-165

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357695

pISSN:

1304-7981

Aedes

salivary protein D7

epitope

vaccine

in silico

Sayı

Makale detayları

In Silico Analyzing 37 kDa Salivary Protein D7 and Discovering Vaccine Candidate and Diagnostic Epitopes to Develop a Transmission-blocking Multi-epitope Vector Vaccine Against Aedes Species (A. aegypti and A. albopictus) Transmitting Several Arboviral Pathogens

Yazarlar: Ahmet Köseoğlu | Buminhan Özgültekin | Yusuf Şeflekçi

Yayıncı: Tokat Gaziosmanpasa University

Ana Başlıklar: Bioinformatics and Computational Biology (Other) | Entomology | Parasitology

Dergi: Journal of New Results in Science Vol.14 Issue 2, pp.152-165

Belge Tipi: Makale

Yayın Tarihi: 8/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001357695

pISSN: 1304-7981

Aedes

salivary protein D7

epitope

vaccine

in silico

Sayı

Yazarlar:

Ahmet Köseoğlu | Buminhan Özgültekin | Yusuf Şeflekçi

Yayıncı:

Tokat Gaziosmanpasa University

Ana Başlıklar:

Bioinformatics and Computational Biology (Other) | Entomology | Parasitology

Dergi:

Journal of New Results in Science Vol.14 Issue 2, pp.152-165

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357695

pISSN:

1304-7981