The protective effects of vitamin D3 on histopathology of pancreas and liver in streptozotocin-induced diabetic rats

Muhamed Focak; Damir Suljevic

Makale detayları

The protective effects of vitamin D3 on histopathology of pancreas and liver in streptozotocin-induced diabetic rats

Yazarlar:

Muhamed Focak | Damir Suljevic

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Sciences

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 2, pp.325-333

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346340

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 The purpose of this study was to explore possible protective effects of vitamin D3 on serum glucose concentration, body weight and histopathology of pancreas and liver. Animals were divided into 3 groups: Control group n6, streptozotocin STZ group n6 and streptozotocin  vitamin D3 STZD3 group n6. Rats in the STZD3 group starting from the 7th day of experiment were given vitamin D3 for 14 days. Glucose levels and body weight were measured on the 1, 7, 14 and 21st day of experiment. Qualitative histological analysis of pancreas and liver was done using the light microscope with a digital camera. Differences between the groups were tested by one-way analysis of variance ANOVA followed by Dunnetts posttest. Differences in repeated measures were tested using paired t-test. On day 14 and 21, blood glucose level in STZD3 group was significantly higher compared to the control group of animals but significantly lower than the glucose level registered in STZ group of rats. On day 14 and day 21, body weight in STZ rats was significantly lower compared to weight in STZD3 and control groups of rats. Morphological changes, such as shrinkage of islets, vacuolation of both endocrine and exocrine cells, were observed in pancreas of STZ group of animals but were nearly absent in STZD3 rats. Similarly, STZD3 group of rats showed preserved liver histoarchitecture. Obtained results suggest that vitamin D3 treatment reduces hyperglycemia, exerts beneficial effects on body weight and alleviates histopathological changes in pancreas and liver in STZ-induced diabetic rats.

 The purpose of this study was to explore possible protective effects of vitamin D3 on serum glucose concentration, body weight and histopathology of pancreas and liver. Animals were divided into 3 groups: Control group n6, streptozotocin STZ group n6 and streptozotocin  vitamin D3 STZD3 group n6. Rats in the STZD3 group starting from the 7th day of experiment were given vitamin D3 for 14 days. Glucose levels and body weight were measured on the 1, 7, 14 and 21st day of experiment. Qualitative histological analysis of pancreas and liver was done using the light microscope with a digital camera. Differences between the groups were tested by one-way analysis of variance ANOVA followed by Dunnetts posttest. Differences in repeated measures were tested using paired t-test. On day 14 and 21, blood glucose level in STZD3 group was significantly higher compared to the control group of animals but significantly lower than the glucose level registered in STZ group of rats. On day 14 and day 21, body weight in STZ rats was significantly lower compared to weight in STZD3 and control groups of rats. Morphological changes, such as shrinkage of islets, vacuolation of both endocrine and exocrine cells, were observed in pancreas of STZ group of animals but were nearly absent in STZD3 rats. Similarly, STZD3 group of rats showed preserved liver histoarchitecture. Obtained results suggest that vitamin D3 treatment reduces hyperglycemia, exerts beneficial effects on body weight and alleviates histopathological changes in pancreas and liver in STZ-induced diabetic rats.

 The purpose of this study was to explore possible protective effects of vitamin D3 on serum glucose concentration, body weight and histopathology of pancreas and liver. Animals were divided into 3 groups: Control group n6, streptozotocin STZ group n6 and streptozotocin  vitamin D3 STZD3 group n6. Rats in the STZD3 group starting from the 7th day of experiment were given vitamin D3 for 14 days. Glucose levels and body weight were measured on the 1, 7, 14 and 21st day of experiment. Qualitative histological analysis of pancreas and liver was done using the light microscope with a digital camera. Differences between the groups were tested by one-way analysis of variance ANOVA followed by Dunnetts posttest. Differences in repeated measures were tested using paired t-test. On day 14 and 21, blood glucose level in STZD3 group was significantly higher compared to the control group of animals but significantly lower than the glucose level registered in STZ group of rats. On day 14 and day 21, body weight in STZ rats was significantly lower compared to weight in STZD3 and control groups of rats. Morphological changes, such as shrinkage of islets, vacuolation of both endocrine and exocrine cells, were observed in pancreas of STZ group of animals but were nearly absent in STZD3 rats. Similarly, STZD3 group of rats showed preserved liver histoarchitecture. Obtained results suggest that vitamin D3 treatment reduces hyperglycemia, exerts beneficial effects on body weight and alleviates histopathological changes in pancreas and liver in STZ-induced diabetic rats.

 The purpose of this study was to explore possible protective effects of vitamin D3 on serum glucose concentration, body weight and histopathology of pancreas and liver. Animals were divided into 3 groups: Control group n6, streptozotocin STZ group n6 and streptozotocin  vitamin D3 STZD3 group n6. Rats in the STZD3 group starting from the 7th day of experiment were given vitamin D3 for 14 days. Glucose levels and body weight were measured on the 1, 7, 14 and 21st day of experiment. Qualitative histological analysis of pancreas and liver was done using the light microscope with a digital camera. Differences between the groups were tested by one-way analysis of variance ANOVA followed by Dunnetts posttest. Differences in repeated measures were tested using paired t-test. On day 14 and 21, blood glucose level in STZD3 group was significantly higher compared to the control group of animals but significantly lower than the glucose level registered in STZ group of rats. On day 14 and day 21, body weight in STZ rats was significantly lower compared to weight in STZD3 and control groups of rats. Morphological changes, such as shrinkage of islets, vacuolation of both endocrine and exocrine cells, were observed in pancreas of STZ group of animals but were nearly absent in STZD3 rats. Similarly, STZD3 group of rats showed preserved liver histoarchitecture. Obtained results suggest that vitamin D3 treatment reduces hyperglycemia, exerts beneficial effects on body weight and alleviates histopathological changes in pancreas and liver in STZ-induced diabetic rats.

Makale detayları

The protective effects of vitamin D3 on histopathology of pancreas and liver in streptozotocin-induced diabetic rats

Yazarlar:

Muhamed Focak | Damir Suljevic

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Sciences

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 2, pp.325-333

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346340

pISSN:

2630-6344

Diabetes mellitus

streptozotocin

vitamin D3

pancreas

liver

Sayı

Makale detayları

The protective effects of vitamin D3 on histopathology of pancreas and liver in streptozotocin-induced diabetic rats

Yazarlar: Muhamed Focak | Damir Suljevic

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Sciences

Dergi: Journal of Research in Pharmacy Vol.26 Issue 2, pp.325-333

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346340

pISSN: 2630-6344

Diabetes mellitus

streptozotocin

vitamin D3

pancreas

liver

Sayı

Yazarlar:

Muhamed Focak | Damir Suljevic

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Sciences

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 2, pp.325-333

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346340

pISSN:

2630-6344