The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats

Aysun TOKER; Fatih AKÇAY; Hülya AKSOY; Halis SÜLEYMAN; Ufuk ÖZGEN; Havva ERDEM

Makale detayları

The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats

Yazarlar:

Aysun TOKER | Fatih AKÇAY | Hülya AKSOY | Halis SÜLEYMAN | Ufuk ÖZGEN | Havva ERDEM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 2, pp.315-320

Belge Tipi:

Makale

Yayın Tarihi:

1/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000753823

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

We investigated the effects of acetyl shikonin isolated from Onosma armeniacum on ethanol-induced gastric injury (ulcer) and on oxidant-antioxidant parameters in the gastric tissue of rats.   Materials and methods: Eighteen albino Wistar male rats were divided into 3 different experimental groups. Group 1 (n = 6) was the control group, group 2 (n = 6) was the ethanol group, and group 3 (n = 6) was the acetyl shikonin + ethanol group. The animals in group 3 were given ethanol (1 mL, 50% v/v) 30 min after acetyl shikonin (40 mg/kg) administration by orogastric tube.   Results: The lowest mean nitric oxide level; the lowest superoxide dismutase, glutathione peroxidase, and glutathione S-transferase activities; and the highest malondialdehyde level and myeloperoxidase activity were obtained in group 2. It was observed that acetyl shikonin increased the activities of antioxidant enzymes and the level of nitric oxide and decreased the level of malondialdehyde and the activity of myeloperoxidase in group 3. In histopathological evaluation, minimal lymphocyte infiltration and edema were seen but no ulcerations were observed in group 3.   Conclusion: The data revealed that the antiulcer effect of acetyl shikonin might be due to an increase in antioxidant enzyme activity and nitric oxide levels, and a decrease in malondialdehyde levels and myeloperoxidase activity.

 

We investigated the effects of acetyl shikonin isolated from Onosma armeniacum on ethanol-induced gastric injury (ulcer) and on oxidant-antioxidant parameters in the gastric tissue of rats.   Materials and methods: Eighteen albino Wistar male rats were divided into 3 different experimental groups. Group 1 (n = 6) was the control group, group 2 (n = 6) was the ethanol group, and group 3 (n = 6) was the acetyl shikonin + ethanol group. The animals in group 3 were given ethanol (1 mL, 50% v/v) 30 min after acetyl shikonin (40 mg/kg) administration by orogastric tube.   Results: The lowest mean nitric oxide level; the lowest superoxide dismutase, glutathione peroxidase, and glutathione S-transferase activities; and the highest malondialdehyde level and myeloperoxidase activity were obtained in group 2. It was observed that acetyl shikonin increased the activities of antioxidant enzymes and the level of nitric oxide and decreased the level of malondialdehyde and the activity of myeloperoxidase in group 3. In histopathological evaluation, minimal lymphocyte infiltration and edema were seen but no ulcerations were observed in group 3.   Conclusion: The data revealed that the antiulcer effect of acetyl shikonin might be due to an increase in antioxidant enzyme activity and nitric oxide levels, and a decrease in malondialdehyde levels and myeloperoxidase activity.

Makale detayları

The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats

Yazarlar:

Aysun TOKER | Fatih AKÇAY | Hülya AKSOY | Halis SÜLEYMAN | Ufuk ÖZGEN | Havva ERDEM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 2, pp.315-320

Belge Tipi:

Makale

Yayın Tarihi:

1/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000753823

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Acetyl shikonin

oxidative stress

ethanol-induced ulcer

Sayı

Makale detayları

The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats

Yazarlar: Aysun TOKER | Fatih AKÇAY | Hülya AKSOY | Halis SÜLEYMAN | Ufuk ÖZGEN | Havva ERDEM

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2013 Vol.43 Issue 2, pp.315-320

Belge Tipi: Makale

Yayın Tarihi: 1/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000753823

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Acetyl shikonin

oxidative stress

ethanol-induced ulcer

Sayı

Yazarlar:

Aysun TOKER | Fatih AKÇAY | Hülya AKSOY | Halis SÜLEYMAN | Ufuk ÖZGEN | Havva ERDEM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 2, pp.315-320

Belge Tipi:

Makale

Yayın Tarihi:

1/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000753823

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144