Evaluation of Total and Lipid-Bound Sialic Acids, Trace and Macro Elements, and Some Biochemical Parameters in Dogs with Babesiosis

Ugur Ozdek; Yeter Değer; Bekir Oğuz

Makale detayları

Evaluation of Total and Lipid-Bound Sialic Acids, Trace and Macro Elements, and Some Biochemical Parameters in Dogs with Babesiosis

Yazarlar:

Ugur Ozdek | Yeter Değer | Bekir Oğuz

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Veteriner Biyokimya

Dergi:

Van Veterinary Journal Vol.34 Issue 3, pp.224-229

Belge Tipi:

Makale

Yayın Tarihi:

11/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001304289

pISSN:

2149-3359

Anahtar kelimeler:

Özet:

 The present study was designed to investigate the changes at the levels of total sialic acid TSA, lipid-bound sialic acid LSA, trace and macroelements, and some biochemical parameters in dogs naturally infected with babesiosis. While babesiosis group consisted of seven dogs which were diagnosed with babesiosis clinically and parasitologically ELISA, control group consisted of seven healthy dogs. Serum TSA and LSA levels in blood samples were measured spectrophotometrically by Sydow and Katapodis methods, respectively. Some biochemical parameters and macroelement measurements were performed using a modular autoanalyzer device. Trace mineral measurements were performed by ICP-MS technique. Compared to the healthy group, dogs with babesiosis had considerably higher TSA and LSA levels. Serum AST, ALP, LDH and CK enzyme activities and CRP, glucose, globulin, total bilirubin, urea, uric acid, creatinine and BUN levels of the babesiosis group significantly increased, while total protein level significantly decreased. The changes in ALT enzyme activity and triglyceride, cholesterol, HDL, LDL, HDL-CDL and ferritin levels were not statistically significant. Zinc, copper, magnesium, sodium, and potassium levels of the babesiosis group decreased significantly, while iron and chlorine levels increased significantly p0.05. Changes in calcium and phosphorus levels were not statistically significant. In conclusion, babesiosis caused significant changes in the levels of sialic acid SA, biochemical parameters and elements in dogs.

 The present study was designed to investigate the changes at the levels of total sialic acid TSA, lipid-bound sialic acid LSA, trace and macroelements, and some biochemical parameters in dogs naturally infected with babesiosis. While babesiosis group consisted of seven dogs which were diagnosed with babesiosis clinically and parasitologically ELISA, control group consisted of seven healthy dogs. Serum TSA and LSA levels in blood samples were measured spectrophotometrically by Sydow and Katapodis methods, respectively. Some biochemical parameters and macroelement measurements were performed using a modular autoanalyzer device. Trace mineral measurements were performed by ICP-MS technique. Compared to the healthy group, dogs with babesiosis had considerably higher TSA and LSA levels. Serum AST, ALP, LDH and CK enzyme activities and CRP, glucose, globulin, total bilirubin, urea, uric acid, creatinine and BUN levels of the babesiosis group significantly increased, while total protein level significantly decreased. The changes in ALT enzyme activity and triglyceride, cholesterol, HDL, LDL, HDL-CDL and ferritin levels were not statistically significant. Zinc, copper, magnesium, sodium, and potassium levels of the babesiosis group decreased significantly, while iron and chlorine levels increased significantly p0.05. Changes in calcium and phosphorus levels were not statistically significant. In conclusion, babesiosis caused significant changes in the levels of sialic acid SA, biochemical parameters and elements in dogs.

 The present study was designed to investigate the changes at the levels of total sialic acid TSA, lipid-bound sialic acid LSA, trace and macroelements, and some biochemical parameters in dogs naturally infected with babesiosis. While babesiosis group consisted of seven dogs which were diagnosed with babesiosis clinically and parasitologically ELISA, control group consisted of seven healthy dogs. Serum TSA and LSA levels in blood samples were measured spectrophotometrically by Sydow and Katapodis methods, respectively. Some biochemical parameters and macroelement measurements were performed using a modular autoanalyzer device. Trace mineral measurements were performed by ICP-MS technique. Compared to the healthy group, dogs with babesiosis had considerably higher TSA and LSA levels. Serum AST, ALP, LDH and CK enzyme activities and CRP, glucose, globulin, total bilirubin, urea, uric acid, creatinine and BUN levels of the babesiosis group significantly increased, while total protein level significantly decreased. The changes in ALT enzyme activity and triglyceride, cholesterol, HDL, LDL, HDL-CDL and ferritin levels were not statistically significant. Zinc, copper, magnesium, sodium, and potassium levels of the babesiosis group decreased significantly, while iron and chlorine levels increased significantly p0.05. Changes in calcium and phosphorus levels were not statistically significant. In conclusion, babesiosis caused significant changes in the levels of sialic acid SA, biochemical parameters and elements in dogs.

 Bu çalışma doğal olarak babesiosis ile enfekte köpeklerde, total sialik asit TSA, lipide-bağlı sialik asit LSA, iz ve makro elementler ile bazı biyokimyasal parametrelerin seviyelerinde meydana gelen değişiklikleri araştırmak için planlandı. Klinik ve parazitolojik olarak ELISA babesiosis tanısı konulan 7adet köpek hasta grubunu, 7 adet sağlıklı köpekte kontrol grubuoluşturdu. Kan örneklerinde serum TSA ve LSA düzeyleri sırasıyla Sydow ve Katapodis metotları ile spektrofotometrik olarak ölçüldü. Bazı biyokimyasal parametre ve makro element ölçümleri modüler oto analizör cihazında gerçekleştirildi. İz mineral ölçümleri ICPMS tekniği ile çalışıldı. Sağlıklı grup ile karşılaştırıldığında babesiosisli köpeklerde serum TSA ve LSA seviyelerinin önemli derecede yüksek olduğu belirlendi p0.05. Babesiosisli grupta serum AST, ALP, LDH ve CK enzim aktiviteleri ile CRP, glukoz, globulin, total bilirubin, üre, ürik asit, kreatinin ve BUN seviyelerinde önemli derecede yükselme, total protein seviyesinde ise önemli düzeyde azalma olduğu saptandı p0.05. ALT enzim aktivitesi ile trigliserit, kolesterol, HDL, LDL, TIBC ve ferritin seviyelerindeki değişikliklerin istatistiki olarak anlamlı olmadığı belirlendi p0.05. Babesiosisli grupta çinko, bakır, magnezyum, sodyum ve potasyum seviyelerinin önemli derecede azaldığı, demir ve klor seviyelerinin ise önemli derecede arttığı tespit edildi p0.05. Kalsiyum ve fosfor seviyelerindeki değişikliklerin istatistiki olarak anlamlı olmadığı belirlendi p0.05. Sonuç olarak, babesiosisin köpeklerde serum sialik asit, biyokimyasal parametreler ve elementlerin seviyelerinde önemli değişikliklere yol açtığı görüldü.

Makale detayları

Evaluation of Total and Lipid-Bound Sialic Acids, Trace and Macro Elements, and Some Biochemical Parameters in Dogs with Babesiosis

Yazarlar:

Ugur Ozdek | Yeter Değer | Bekir Oğuz

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Veteriner Biyokimya

Dergi:

Van Veterinary Journal Vol.34 Issue 3, pp.224-229

Belge Tipi:

Makale

Yayın Tarihi:

11/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001304289

pISSN:

2149-3359

Biomarker

Dogs

Minerals

Sialic acidsBabeziyoz

Biyobelirteçler

Köpek

Mineraller

Sialikasidler

Sayı

Makale detayları

Yazarlar: Ugur Ozdek | Yeter Değer | Bekir Oğuz

Yayıncı: Van Yuzuncu Yıl University

Ana Başlıklar: Veteriner Biyokimya

Dergi: Van Veterinary Journal Vol.34 Issue 3, pp.224-229

Belge Tipi: Makale

Yayın Tarihi: 11/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001304289

pISSN: 2149-3359

Sayı

Yazarlar:

Ugur Ozdek | Yeter Değer | Bekir Oğuz

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Veteriner Biyokimya

Dergi:

Van Veterinary Journal Vol.34 Issue 3, pp.224-229

Belge Tipi:

Makale

Yayın Tarihi:

11/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001304289

pISSN:

2149-3359