Molecular Cloning and Expression Analysis of Extra Sex Combs Gene  during Development in Macrobrachium nipponense

Yanping ZHANG; Sufei JİANG; Yiwei XİONG; Shengming SUN; Hui QİAO; Shubo JİN; Yongsheng GONG; Hongtuo FU

Makale detayları

Molecular Cloning and Expression Analysis of Extra Sex Combs Gene during Development in Macrobrachium nipponense

Yazarlar:

Yanping ZHANG | Sufei JİANG | Yiwei XİONG | Shengming SUN | Hui QİAO | Shubo JİN | Yongsheng GONG | Hongtuo FU

Yayıncı:

Trabzon Su Ürünleri Merkez Araştırma Enstitüsü

Dergi:

Turkish Journal of Fisheries and Aquatic Sciences 2013 Vol.13 Issue 2

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000838218

Sıklık:

Üç Aylık

pISSN:

1303-2712

Anahtar kelimeler:

Özet:

 

The extra sex combs (Esc) gene first identified in Drosophia encodes a transcriptional repressor of homeotic genes  belonging to the polycomb group (PcG) gene and is primarily expressed in the early embryos. In this study, we have isolated  the full-length cDNA of an Esc gene from the testis of oriental river prawn (Macmbrachium nipponense) according to the  established expressed sequence tags (ESTs) information using Rapid Amplification of the cDNA Ends (RACE) technique,  designated as MnEsc. The full-length cDNA of MnEsc is 1,461bp in size and has an open reading frame (ORF) of 1,068bp,  encoding a 355-amino acid protein. Real-time quantitative PCR analyses revealed that the expression level of MnEsc varied  significantly in the developing embryo, postembryonic larval and adult tissues. Real-time quantitative PCR showed the  MnEsc gene was expressed in testis, liver, ovary, brain, abdominal ganglion, heart, intestine and muscle with the highest level  of expression in ovary and brain. In vivo silencing of the gene, the dsRNA of MnEsc caused a significantly decrease in target  gene expression level in brain and ovary tissues, but no exterior appearance change of experimental shrimp was observed. 	 	 		 .

 

The extra sex combs (Esc) gene first identified in Drosophia encodes a transcriptional repressor of homeotic genes  belonging to the polycomb group (PcG) gene and is primarily expressed in the early embryos. In this study, we have isolated  the full-length cDNA of an Esc gene from the testis of oriental river prawn (Macmbrachium nipponense) according to the  established expressed sequence tags (ESTs) information using Rapid Amplification of the cDNA Ends (RACE) technique,  designated as MnEsc. The full-length cDNA of MnEsc is 1,461bp in size and has an open reading frame (ORF) of 1,068bp,  encoding a 355-amino acid protein. Real-time quantitative PCR analyses revealed that the expression level of MnEsc varied  significantly in the developing embryo, postembryonic larval and adult tissues. Real-time quantitative PCR showed the  MnEsc gene was expressed in testis, liver, ovary, brain, abdominal ganglion, heart, intestine and muscle with the highest level  of expression in ovary and brain. In vivo silencing of the gene, the dsRNA of MnEsc caused a significantly decrease in target  gene expression level in brain and ovary tissues, but no exterior appearance change of experimental shrimp was observed. 	 	 		 .

Makale detayları

Molecular Cloning and Expression Analysis of Extra Sex Combs Gene during Development in Macrobrachium nipponense

Yazarlar:

Yanping ZHANG | Sufei JİANG | Yiwei XİONG | Shengming SUN | Hui QİAO | Shubo JİN | Yongsheng GONG | Hongtuo FU

Yayıncı:

Trabzon Su Ürünleri Merkez Araştırma Enstitüsü

Dergi:

Turkish Journal of Fisheries and Aquatic Sciences 2013 Vol.13 Issue 2

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000838218

Sıklık:

Üç Aylık

pISSN:

1303-2712

Macrobrachium nipponense

extra sex combs

real-time quantitative PCR

RNAi

Sayı

Makale detayları

Molecular Cloning and Expression Analysis of Extra Sex Combs Gene during Development in Macrobrachium nipponense

Yazarlar: Yanping ZHANG | Sufei JİANG | Yiwei XİONG | Shengming SUN | Hui QİAO | Shubo JİN | Yongsheng GONG | Hongtuo FU

Yayıncı: Trabzon Su Ürünleri Merkez Araştırma Enstitüsü

Dergi: Turkish Journal of Fisheries and Aquatic Sciences 2013 Vol.13 Issue 2

Belge Tipi: Makale

Yayın Tarihi: 3/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000838218

Sıklık: Üç Aylık

pISSN: 1303-2712

Macrobrachium nipponense

extra sex combs

real-time quantitative PCR

RNAi

Sayı

Yazarlar:

Yanping ZHANG | Sufei JİANG | Yiwei XİONG | Shengming SUN | Hui QİAO | Shubo JİN | Yongsheng GONG | Hongtuo FU

Yayıncı:

Trabzon Su Ürünleri Merkez Araştırma Enstitüsü

Dergi:

Turkish Journal of Fisheries and Aquatic Sciences 2013 Vol.13 Issue 2

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000838218

Sıklık:

Üç Aylık

pISSN:

1303-2712