Production of recombinant expansin and detection by SDS page analysis in Escherichia coli

Serap Güneş; Demet Ektiren; Mehmet Karaaslan; Hasan Vardin

Makale detayları

Production of recombinant expansin and detection by SDS page analysis in Escherichia coli

Yazarlar:

Serap Güneş | Demet Ektiren | Mehmet Karaaslan | Hasan Vardin

Yayıncı:

Gültekin ÖZDEMİR

Ana Başlıklar:

Food Engineering

Dergi:

International Journal of Agriculture Environment and Food Sciences Vol.7 Issue 1, pp.117-121

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282196

pISSN:

2618-5946

Anahtar kelimeler:

Özet:

 The study aims to produce Expansin protein isolated from a young tomato plant by using Escherichia coli which is used in recombinant protein production. Continuous culture is the most common method used to grow cells for recombinant protein production. In the study, the K12 strain of E. coli was used as a culture for the production of Expansin protein. The used LeExp1 gene was isolated from a young tomato plant. Since the related gene is found in very small amounts in plants, it has been reproduced using the PCR method and has been made workable with this method. T17 vector T7 RNA polymerase system, which is frequently used in the production of recombinant protein, was used as the bacterial expression vector. The T7 RNA polymerase system is a commonly used vector in E. coli. With the transfer, the E. coli bacterium was given the ability to produce recombinant protein. Whether the obtained recombinant protein expressed the appropriate protein was determined by SDS Page analysis.

 The study aims to produce Expansin protein isolated from a young tomato plant by using Escherichia coli which is used in recombinant protein production. Continuous culture is the most common method used to grow cells for recombinant protein production. In the study, the K12 strain of E. coli was used as a culture for the production of Expansin protein. The used LeExp1 gene was isolated from a young tomato plant. Since the related gene is found in very small amounts in plants, it has been reproduced using the PCR method and has been made workable with this method. T17 vector T7 RNA polymerase system, which is frequently used in the production of recombinant protein, was used as the bacterial expression vector. The T7 RNA polymerase system is a commonly used vector in E. coli. With the transfer, the E. coli bacterium was given the ability to produce recombinant protein. Whether the obtained recombinant protein expressed the appropriate protein was determined by SDS Page analysis.

 The study aims to produce Expansin protein isolated from a young tomato plant by using Escherichia coli which is used in recombinant protein production. Continuous culture is the most common method used to grow cells for recombinant protein production. In the study, the K12 strain of E. coli was used as a culture for the production of Expansin protein. The used LeExp1 gene was isolated from a young tomato plant. Since the related gene is found in very small amounts in plants, it has been reproduced using the PCR method and has been made workable with this method. T17 vector T7 RNA polymerase system, which is frequently used in the production of recombinant protein, was used as the bacterial expression vector. The T7 RNA polymerase system is a commonly used vector in E. coli. With the transfer, the E. coli bacterium was given the ability to produce recombinant protein. Whether the obtained recombinant protein expressed the appropriate protein was determined by SDS Page analysis.

 The study aims to produce Expansin protein isolated from a young tomato plant by using Escherichia coli which is used in recombinant protein production. Continuous culture is the most common method used to grow cells for recombinant protein production. In the study, the K12 strain of E. coli was used as a culture for the production of Expansin protein. The used LeExp1 gene was isolated from a young tomato plant. Since the related gene is found in very small amounts in plants, it has been reproduced using the PCR method and has been made workable with this method. T17 vector T7 RNA polymerase system, which is frequently used in the production of recombinant protein, was used as the bacterial expression vector. The T7 RNA polymerase system is a commonly used vector in E. coli. With the transfer, the E. coli bacterium was given the ability to produce recombinant protein. Whether the obtained recombinant protein expressed the appropriate protein was determined by SDS Page analysis.

Makale detayları

Production of recombinant expansin and detection by SDS page analysis in Escherichia coli

Yazarlar:

Serap Güneş | Demet Ektiren | Mehmet Karaaslan | Hasan Vardin

Yayıncı:

Gültekin ÖZDEMİR

Ana Başlıklar:

Food Engineering

Dergi:

International Journal of Agriculture Environment and Food Sciences Vol.7 Issue 1, pp.117-121

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282196

pISSN:

2618-5946

Recombinant protein

Expansin protein

LeExp1 gene

Escherichia coli

PCR

Sayı

Makale detayları

Production of recombinant expansin and detection by SDS page analysis in Escherichia coli

Yazarlar: Serap Güneş | Demet Ektiren | Mehmet Karaaslan | Hasan Vardin

Yayıncı: Gültekin ÖZDEMİR

Ana Başlıklar: Food Engineering

Dergi: International Journal of Agriculture Environment and Food Sciences Vol.7 Issue 1, pp.117-121

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001282196

pISSN: 2618-5946

Recombinant protein

Expansin protein

LeExp1 gene

Escherichia coli

PCR

Sayı

Yazarlar:

Serap Güneş | Demet Ektiren | Mehmet Karaaslan | Hasan Vardin

Yayıncı:

Gültekin ÖZDEMİR

Ana Başlıklar:

Food Engineering

Dergi:

International Journal of Agriculture Environment and Food Sciences Vol.7 Issue 1, pp.117-121

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282196

pISSN:

2618-5946