Stereological Examination of Parietal and Temporal Regions of the Brain in Rats Induced with Epilepsy with Pentylenetetrazole

Veysel Akyol; Gamze Çakmak

Makale detayları

Stereological Examination of Parietal and Temporal Regions of the Brain in Rats Induced with Epilepsy with Pentylenetetrazole

Yazarlar:

Veysel Akyol | Gamze Çakmak

Yayıncı:

Kyrgyz-Turkish Manas University

Ana Başlıklar:

Veteriner Anatomi ve Fizyoloji

Dergi:

Manas Journal of Agriculture Veterinary and Life Sciences Vol.14 Issue 2, pp.187-200

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001335383

pISSN:

1694-7932

Anahtar kelimeler:

Özet:

 The aim of this study was to investigate the parietal and temporal regions of the brain stereologically after epilepsy induced by pentylenetetrazole PTZ. 20 healthy male Wistar Albino rats, 3 months old, weighing approximately 250 gr, were chosen for the study. The rats were divided into PTZ and control groups, 10 in each group. 80 mgkg PTZ was mixed into 0.5 ml of 0.9 physiological saline and administered intraperitoneally as a single dose injection to the PTZ group. Behavioral changes specified in six stages were determined by following the Racine scale within a 30-minute observation period. After perfusion, the rats brains were removed. After tissue tracking, an average of 10 sections were obtained for each animal, with a thickness of 175 of 5µm. Sections were stained with Hematoxylin-Eosin. The preparations were photographed with a 1.25 objective. For each brain tissue, the total lobus parietalis, lobus temporalis, the grey matter volume and the total number of neurons in the lobus parietalis and lobus temporalis were calculated. Mann-Whitney U test was used for statistical analysis IBM SPSS for Windows, ver.25 No statistically significant difference could be detected in the total lobus parietalis, lobus temporalis and the grey matter volume, volume values of the brain and the number of neurons determined between the groups p0.05. However, when evaluated according to groups, a significant difference was obtained in the volume ratio values, the grey matter volumetotal lobus parietalis and the grey matter volumetotal lobus temporalis ratio p0.05.

 The aim of this study was to investigate the parietal and temporal regions of the brain stereologically after epilepsy induced by pentylenetetrazole PTZ. 20 healthy male Wistar Albino rats, 3 months old, weighing approximately 250 gr, were chosen for the study. The rats were divided into PTZ and control groups, 10 in each group. 80 mgkg PTZ was mixed into 0.5 ml of 0.9 physiological saline and administered intraperitoneally as a single dose injection to the PTZ group. Behavioral changes specified in six stages were determined by following the Racine scale within a 30-minute observation period. After perfusion, the rats brains were removed. After tissue tracking, an average of 10 sections were obtained for each animal, with a thickness of 175 of 5µm. Sections were stained with Hematoxylin-Eosin. The preparations were photographed with a 1.25 objective. For each brain tissue, the total lobus parietalis, lobus temporalis, the grey matter volume and the total number of neurons in the lobus parietalis and lobus temporalis were calculated. Mann-Whitney U test was used for statistical analysis IBM SPSS for Windows, ver.25 No statistically significant difference could be detected in the total lobus parietalis, lobus temporalis and the grey matter volume, volume values of the brain and the number of neurons determined between the groups p0.05. However, when evaluated according to groups, a significant difference was obtained in the volume ratio values, the grey matter volumetotal lobus parietalis and the grey matter volumetotal lobus temporalis ratio p0.05.

 The aim of this study was to investigate the parietal and temporal regions of the brain stereologically after epilepsy induced by pentylenetetrazole PTZ. 20 healthy male Wistar Albino rats, 3 months old, weighing approximately 250 gr, were chosen for the study. The rats were divided into PTZ and control groups, 10 in each group. 80 mgkg PTZ was mixed into 0.5 ml of 0.9 physiological saline and administered intraperitoneally as a single dose injection to the PTZ group. Behavioral changes specified in six stages were determined by following the Racine scale within a 30-minute observation period. After perfusion, the rats brains were removed. After tissue tracking, an average of 10 sections were obtained for each animal, with a thickness of 175 of 5µm. Sections were stained with Hematoxylin-Eosin. The preparations were photographed with a 1.25 objective. For each brain tissue, the total lobus parietalis, lobus temporalis, the grey matter volume and the total number of neurons in the lobus parietalis and lobus temporalis were calculated. Mann-Whitney U test was used for statistical analysis IBM SPSS for Windows, ver.25 No statistically significant difference could be detected in the total lobus parietalis, lobus temporalis and the grey matter volume, volume values of the brain and the number of neurons determined between the groups p0.05. However, when evaluated according to groups, a significant difference was obtained in the volume ratio values, the grey matter volumetotal lobus parietalis and the grey matter volumetotal lobus temporalis ratio p0.05.

 Bu çalışmanın amacı, pentilentetrazol PTZ ile oluşturulan epilepsi sonrası beynin pariyetal ve temporal bölgelerini stereolojik olarak incelemektir. Çalışma için yaklaşık 250 gr ağırlığında, 3 aylık, 20 adet sağlıklı erkek Wistar Albino sıçan seçildi. Sıçanlar her grupta 10 adet olacak şekilde PTZ ve kontrol gruplarına ayrıldı. 80 mgkg PTZ, 0.5 ml 0.9 fizyolojik salin içerisine karıştırıldı ve PTZ grubuna tek doz enjeksiyon halinde intraperitoneal olarak uygulandı. Altı aşamada belirlenen davranış değişiklikleri, 30 dakikalık gözlem süresi içerisinde Racine skalası takip edilerek belirlendi. Perfüzyonun ardından sıçanların beyinleri çıkarıldı. Doku takibi sonrasında her hayvan için 175 5 µm kalınlığında ortalama 10 adet kesit elde edildi. Kesitler Hematoksilen-Eozin ile boyanarak 1.25 objektifle fotoğraflandı. Her beyin dokusu için lobus parietalis, lobus temporalis, gri madde hacmi ve lobus parietalis ve lobus temporalisteki toplam nöron sayısı hesaplandı. İstatistiksel analizde Mann-Whitney U testi kullanıldı IBM SPSS for Windows, ver.25.Lobus parietalis, lobus temporalis ve beyindeki gri madde hacmi, hacim değerleri ve gruplar arasında nöron sayısı değerlerinde istatistiksel olarak anlamlı bir fark saptanmadı. p0.05. Ancak gruplara göre değerlendirildiğinde hacim oranı değerlerinde yani gri madde hacmitoplam lobus parietalis ve gri madde hacmitoplam lobus temporalis oranında anlamlı farklılık elde edildi p0.05.

Makale detayları

Stereological Examination of Parietal and Temporal Regions of the Brain in Rats Induced with Epilepsy with Pentylenetetrazole

Yazarlar:

Veysel Akyol | Gamze Çakmak

Yayıncı:

Kyrgyz-Turkish Manas University

Ana Başlıklar:

Veteriner Anatomi ve Fizyoloji

Dergi:

Manas Journal of Agriculture Veterinary and Life Sciences Vol.14 Issue 2, pp.187-200

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001335383

pISSN:

1694-7932

Epilepsy

Parietal lobe

Pentylenetetrazole (PTZ)

Stereology

Temporal lobe.Epilepsi

Parietal lob

Pentylenetetrazol (PTZ)

Stereoloji

Temporal lob.

Sayı

Makale detayları

Yazarlar: Veysel Akyol | Gamze Çakmak

Yayıncı: Kyrgyz-Turkish Manas University

Ana Başlıklar: Veteriner Anatomi ve Fizyoloji

Dergi: Manas Journal of Agriculture Veterinary and Life Sciences Vol.14 Issue 2, pp.187-200

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001335383

pISSN: 1694-7932

Sayı

Yazarlar:

Veysel Akyol | Gamze Çakmak

Yayıncı:

Kyrgyz-Turkish Manas University

Ana Başlıklar:

Veteriner Anatomi ve Fizyoloji

Dergi:

Manas Journal of Agriculture Veterinary and Life Sciences Vol.14 Issue 2, pp.187-200

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001335383

pISSN:

1694-7932