A loop-mediated isothermal amplification (LAMP) assay for rapid detection of Anaplasma phagocytophilum infection in dogs

Chung-Chan LEE; Yung-Chang LIN; Chau-Loong TSANG; Yang-Tsung CHUNG

Makale detayları

A loop-mediated isothermal amplification (LAMP) assay for rapid detection of Anaplasma phagocytophilum infection in dogs

Yazarlar:

Chung-Chan LEE | Yung-Chang LIN | Chau-Loong TSANG | Yang-Tsung CHUNG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2012 Vol.36 Issue 2, pp.205-210

Belge Tipi:

Makale

Yayın Tarihi:

1/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000767006

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

A simple and rapid diagnostic method of Anaplasma phagocytophilum infection was established, employing the loop-mediated isothermal amplification (LAMP) reaction. With 1 set of 4 specific primers targeting the citrate synthase (gltA) gene of A. phagocytophilum, the LAMP assay rapidly amplified the target DNA sequence in a single tube in 1 h, requiring only a water bath for reaction. When visualized by gel electrophoresis, the LAMP products appeared as a ladder-like pattern, with many bands of different sizes from 180 base pairs up to the loading well. The results obtained from testing 32 clinical blood samples of dogs demonstrated that the LAMP method was more sensitive than nested PCR in the diagnosis reaction for the detection of A. phagocytophilum DNA.

 

A simple and rapid diagnostic method of Anaplasma phagocytophilum infection was established, employing the loop-mediated isothermal amplification (LAMP) reaction. With 1 set of 4 specific primers targeting the citrate synthase (gltA) gene of A. phagocytophilum, the LAMP assay rapidly amplified the target DNA sequence in a single tube in 1 h, requiring only a water bath for reaction. When visualized by gel electrophoresis, the LAMP products appeared as a ladder-like pattern, with many bands of different sizes from 180 base pairs up to the loading well. The results obtained from testing 32 clinical blood samples of dogs demonstrated that the LAMP method was more sensitive than nested PCR in the diagnosis reaction for the detection of A. phagocytophilum DNA.

Makale detayları

A loop-mediated isothermal amplification (LAMP) assay for rapid detection of Anaplasma phagocytophilum infection in dogs

Yazarlar:

Chung-Chan LEE | Yung-Chang LIN | Chau-Loong TSANG | Yang-Tsung CHUNG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2012 Vol.36 Issue 2, pp.205-210

Belge Tipi:

Makale

Yayın Tarihi:

1/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000767006

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Key words: Anaplasma phagocytophilum

gltA gene

LAMP assay

dogs

Sayı

Makale detayları

A loop-mediated isothermal amplification (LAMP) assay for rapid detection of Anaplasma phagocytophilum infection in dogs

Yazarlar: Chung-Chan LEE | Yung-Chang LIN | Chau-Loong TSANG | Yang-Tsung CHUNG

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2012 Vol.36 Issue 2, pp.205-210

Belge Tipi: Makale

Yayın Tarihi: 1/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000767006

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Key words: Anaplasma phagocytophilum

gltA gene

LAMP assay

dogs

Sayı

Yazarlar:

Chung-Chan LEE | Yung-Chang LIN | Chau-Loong TSANG | Yang-Tsung CHUNG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2012 Vol.36 Issue 2, pp.205-210

Belge Tipi:

Makale

Yayın Tarihi:

1/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000767006

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128