Morphological and molecular identification and determination of host status of Cuscuta campestris Yunck. in Thrace Region of Türkiye

Bahadır Şin; Lerzan Öztürk; Zeliha Orhan Özalp; İzzet Kadıoğlu

Makale detayları

Morphological and molecular identification and determination of host status of Cuscuta campestris Yunck. in Thrace Region of Türkiye

Yazarlar:

Bahadır Şin | Lerzan Öztürk | Zeliha Orhan Özalp | İzzet Kadıoğlu

Yayıncı:

Hatay Mustafa Kemal University

Ana Başlıklar:

Bitki Koruma (Diğer)

Dergi:

Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi Vol.29 Issue 3, pp.755-768

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001332643

pISSN:

2667-7733

Anahtar kelimeler:

Özet:

 Field dodder Cuscuta campestris Yunck. is a weed species that parasitizes many cultivated plants and has been reported in 40 provinces in Türkiye. Despite being found on so many plants, cases of parasitism in the Poaceae family are limited worldwide and there is no evidence in Türkiye. In this study, weed parasitism was observed in monocot plants of the Poaceae family such as Setaria viridis, Sorghum halepense, Alopecurus myosuroides, Avena fatua, Avena sterilis, Eleusine indica, Echinochloa crus-galli, Bromus tectorum, Hordeum murinum, Elymus repens, Lolium perenne, Secale cereale and Triticum aestivum growing in Thrace Region in Northwestern part of Türkiye. According to morphological identification parameters, the parasitic weed was identified as C. campestris. C. campestris was observed to cause high damage to Echinochloa crus-galli and Sorghum halepense weed species. A molecular study was conducted to identify the weed on a molecular basis and analyze its molecular phylogeny. For this purpose, DNA was extracted, amplified with specific primers, sequenced and subjected to GenBank sequence comparison using BLAST. In BLAST, the local population showed higher similarity 99.13 with other C. campestris accessions KJ400050 and EU883527 and clustered in the closest class with the same species in the Maximum Likelihood tree generated using Mega 7 software.

 Field dodder Cuscuta campestris Yunck. is a weed species that parasitizes many cultivated plants and has been reported in 40 provinces in Türkiye. Despite being found on so many plants, cases of parasitism in the Poaceae family are limited worldwide and there is no evidence in Türkiye. In this study, weed parasitism was observed in monocot plants of the Poaceae family such as Setaria viridis, Sorghum halepense, Alopecurus myosuroides, Avena fatua, Avena sterilis, Eleusine indica, Echinochloa crus-galli, Bromus tectorum, Hordeum murinum, Elymus repens, Lolium perenne, Secale cereale and Triticum aestivum growing in Thrace Region in Northwestern part of Türkiye. According to morphological identification parameters, the parasitic weed was identified as C. campestris. C. campestris was observed to cause high damage to Echinochloa crus-galli and Sorghum halepense weed species. A molecular study was conducted to identify the weed on a molecular basis and analyze its molecular phylogeny. For this purpose, DNA was extracted, amplified with specific primers, sequenced and subjected to GenBank sequence comparison using BLAST. In BLAST, the local population showed higher similarity 99.13 with other C. campestris accessions KJ400050 and EU883527 and clustered in the closest class with the same species in the Maximum Likelihood tree generated using Mega 7 software.

 Field dodder Cuscuta campestris Yunck. is a weed species that parasitizes many cultivated plants and has been reported in 40 provinces in Türkiye. Despite being found on so many plants, cases of parasitism in the Poaceae family are limited worldwide and there is no evidence in Türkiye. In this study, weed parasitism was observed in monocot plants of the Poaceae family such as Setaria viridis, Sorghum halepense, Alopecurus myosuroides, Avena fatua, Avena sterilis, Eleusine indica, Echinochloa crus-galli, Bromus tectorum, Hordeum murinum, Elymus repens, Lolium perenne, Secale cereale and Triticum aestivum growing in Thrace Region in Northwestern part of Türkiye. According to morphological identification parameters, the parasitic weed was identified as C. campestris. C. campestris was observed to cause high damage to Echinochloa crus-galli and Sorghum halepense weed species. A molecular study was conducted to identify the weed on a molecular basis and analyze its molecular phylogeny. For this purpose, DNA was extracted, amplified with specific primers, sequenced and subjected to GenBank sequence comparison using BLAST. In BLAST, the local population showed higher similarity 99.13 with other C. campestris accessions KJ400050 and EU883527 and clustered in the closest class with the same species in the Maximum Likelihood tree generated using Mega 7 software.

 Tarla küskütü Cuscuta campestris Yunck. birçok kültür bitkisinde parazit olan bir yabancı ot türüdür ve Türkiyede 40 ilde rapor edilmiştir. Bu kadar çok bitkide bulunmasına rağmen Poaceae familyasında parazitizm vakaları dünya çapında sınırlı olup, Türkiyede de herhangi bir kanıt bulunmamaktadır. Türkiyenin kuzeybatısındaki Trakya bölgesinde yetişen Setaria viridis, Sorghum halepense, Alopecurus myosuroides, Avena fatua, Avena sterilis, Eleusine indica, Echinochloa crus-galli, Bromus tectorum, Hordeum murinum, Elymus repens, Lolium perenne, Secale cereale ve Triticum aestivum dahil olmak üzere Poaceae familyasındaki monokotiledon bitkilerinde küskütün parazit olduğu gözlemlenmiştir. Morfolojik tanımlama parametrelerine göre parazitik olan yabancı ot C. campestris olarak tanımlanmıştır. C. campestrisin Echinochloa crus-galli ve Sorghum halepense yabancı ot türlerinde yüksek oranda zarar meydana getirdiği gözlenmiştir. Yabancı otun moleküler bazda tanımlanması ve moleküler filogenisinin analiz edilmesi amacıyla moleküler çalışma yürütülmüştür. Bu amaçla küsküt DNAsı ekstrakte edilmiş, spesifik primerlerle amplifiye edilmiş, sekanslans yapılmış ve BLAST kullanılarak GenBank sekans karşılaştırmasına tabi tutulmuştur. Blastnda yerel popülasyon, diğer C. campestris aksesyonları KJ400050 ve EU883527 ile daha yüksek benzerlik 99.13 göstermiş ve Mega 7 yazılımı kullanılarak oluşturulan Maksimum Likelihood ağacında aynı türlerle en yakın sınıfta kümelenmiştir.

Makale detayları

Morphological and molecular identification and determination of host status of Cuscuta campestris Yunck. in Thrace Region of Türkiye

Yazarlar:

Bahadır Şin | Lerzan Öztürk | Zeliha Orhan Özalp | İzzet Kadıoğlu

Yayıncı:

Hatay Mustafa Kemal University

Ana Başlıklar:

Bitki Koruma (Diğer)

Dergi:

Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi Vol.29 Issue 3, pp.755-768

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001332643

pISSN:

2667-7733

Field dodder

molecular identification

host plants

distribution

TürkiyeTarla küskütü

moleküler teşhis

konukçu bitkiler

yaygınlık

Türkiye

Sayı

Makale detayları

Yazarlar: Bahadır Şin | Lerzan Öztürk | Zeliha Orhan Özalp | İzzet Kadıoğlu

Yayıncı: Hatay Mustafa Kemal University

Ana Başlıklar: Bitki Koruma (Diğer)

Dergi: Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi Vol.29 Issue 3, pp.755-768

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001332643

pISSN: 2667-7733

Sayı

Yazarlar:

Bahadır Şin | Lerzan Öztürk | Zeliha Orhan Özalp | İzzet Kadıoğlu

Yayıncı:

Hatay Mustafa Kemal University

Ana Başlıklar:

Bitki Koruma (Diğer)

Dergi:

Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi Vol.29 Issue 3, pp.755-768

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001332643

pISSN:

2667-7733