Effect of Transport Condition on the Structural Integrity of Ovarian Tissue and the Development of Sheep Embryos In-Vitro

Saıf Al-hafedh; Yahy Abood Kareem Alesawi; Fatin Cedden

Makale detayları

Effect of Transport Condition on the Structural Integrity of Ovarian Tissue and the Development of Sheep Embryos In-Vitro

Yazarlar:

Saıf Al-hafedh | Yahy Abood Kareem Alesawi | Fatin Cedden

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Veterinary Sciences (Other)

Dergi:

Yuzuncu Yıl University Journal of Agricultural Sciences Vol.34 Issue 3, pp.505-514

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326934

pISSN:

1308-7576

Anahtar kelimeler:

Özet:

 The oocyte quality decreases during ovarian tissue transport to the laboratories of in vitro embryo production. To provide additional information on how the conditions of transporting sheep ovaries impact the ovarian tissue and oocytes ability to develop into blastocyst stages, we have studied new transport media Ankara University Zootekni AUZ1, AUZ2 supplemented with antioxidants melatonin, Vit E, and Vit A, buffer solution, and energy substrates, and compared them with the traditional transport media: Phosphate-Buffered Saline PBS, and Charles Rosenkrans 1 CR1, Normal Saline NS at different temperatures -6 to 30 C. We also studied and compared how well different transport media preserve the ovarian tissues structural integrity while transporting sheep ovaries at 4C. Our findings indicated that various temperatures and transport media play critical roles in embryo development. The embryo development rates showed that when sheep ovaries are transported in AUZ1, they produce oocytes with a higher embryo development rate than other transport media at any temperature. In addition, histology examination revealed that the transport of sheep ovarian tissue in any medium at a temperature of 4 C did not negatively impact the viability and histomorphology of the primordial, primary, and secondary follicles. In contrast to other transport media, the AUZ1 medium maintained the normal morphology of antral follicles, Graafian follicles, and the cumulus oophorus of sheep ovarian tissue. In conclusion, adding melatonin, buffer solution, and energy substrates to the transportation medium of ovarian tissues has a beneficial and positive role in maintaining ovarian tissue and increasing the rates of embryonic development.

 The oocyte quality decreases during ovarian tissue transport to the laboratories of in vitro embryo production. To provide additional information on how the conditions of transporting sheep ovaries impact the ovarian tissue and oocytes ability to develop into blastocyst stages, we have studied new transport media Ankara University Zootekni AUZ1, AUZ2 supplemented with antioxidants melatonin, Vit E, and Vit A, buffer solution, and energy substrates, and compared them with the traditional transport media: Phosphate-Buffered Saline PBS, and Charles Rosenkrans 1 CR1, Normal Saline NS at different temperatures -6 to 30 C. We also studied and compared how well different transport media preserve the ovarian tissues structural integrity while transporting sheep ovaries at 4C. Our findings indicated that various temperatures and transport media play critical roles in embryo development. The embryo development rates showed that when sheep ovaries are transported in AUZ1, they produce oocytes with a higher embryo development rate than other transport media at any temperature. In addition, histology examination revealed that the transport of sheep ovarian tissue in any medium at a temperature of 4 C did not negatively impact the viability and histomorphology of the primordial, primary, and secondary follicles. In contrast to other transport media, the AUZ1 medium maintained the normal morphology of antral follicles, Graafian follicles, and the cumulus oophorus of sheep ovarian tissue. In conclusion, adding melatonin, buffer solution, and energy substrates to the transportation medium of ovarian tissues has a beneficial and positive role in maintaining ovarian tissue and increasing the rates of embryonic development.

 The oocyte quality decreases during ovarian tissue transport to the laboratories of in vitro embryo production. To provide additional information on how the conditions of transporting sheep ovaries impact the ovarian tissue and oocytes ability to develop into blastocyst stages, we have studied new transport media Ankara University Zootekni AUZ1, AUZ2 supplemented with antioxidants melatonin, Vit E, and Vit A, buffer solution, and energy substrates, and compared them with the traditional transport media: Phosphate-Buffered Saline PBS, and Charles Rosenkrans 1 CR1, Normal Saline NS at different temperatures -6 to 30 C. We also studied and compared how well different transport media preserve the ovarian tissues structural integrity while transporting sheep ovaries at 4C. Our findings indicated that various temperatures and transport media play critical roles in embryo development. The embryo development rates showed that when sheep ovaries are transported in AUZ1, they produce oocytes with a higher embryo development rate than other transport media at any temperature. In addition, histology examination revealed that the transport of sheep ovarian tissue in any medium at a temperature of 4 C did not negatively impact the viability and histomorphology of the primordial, primary, and secondary follicles. In contrast to other transport media, the AUZ1 medium maintained the normal morphology of antral follicles, Graafian follicles, and the cumulus oophorus of sheep ovarian tissue. In conclusion, adding melatonin, buffer solution, and energy substrates to the transportation medium of ovarian tissues has a beneficial and positive role in maintaining ovarian tissue and increasing the rates of embryonic development.

 The oocyte quality decreases during ovarian tissue transport to the laboratories of in vitro embryo production. To provide additional information on how the conditions of transporting sheep ovaries impact the ovarian tissue and oocytes ability to develop into blastocyst stages, we have studied new transport media Ankara University Zootekni AUZ1, AUZ2 supplemented with antioxidants melatonin, Vit E, and Vit A, buffer solution, and energy substrates, and compared them with the traditional transport media: Phosphate-Buffered Saline PBS, and Charles Rosenkrans 1 CR1, Normal Saline NS at different temperatures -6 to 30 C. We also studied and compared how well different transport media preserve the ovarian tissues structural integrity while transporting sheep ovaries at 4C. Our findings indicated that various temperatures and transport media play critical roles in embryo development. The embryo development rates showed that when sheep ovaries are transported in AUZ1, they produce oocytes with a higher embryo development rate than other transport media at any temperature. In addition, histology examination revealed that the transport of sheep ovarian tissue in any medium at a temperature of 4 C did not negatively impact the viability and histomorphology of the primordial, primary, and secondary follicles. In contrast to other transport media, the AUZ1 medium maintained the normal morphology of antral follicles, Graafian follicles, and the cumulus oophorus of sheep ovarian tissue. In conclusion, adding melatonin, buffer solution, and energy substrates to the transportation medium of ovarian tissues has a beneficial and positive role in maintaining ovarian tissue and increasing the rates of embryonic development.

Makale detayları

Effect of Transport Condition on the Structural Integrity of Ovarian Tissue and the Development of Sheep Embryos In-Vitro

Yazarlar:

Saıf Al-hafedh | Yahy Abood Kareem Alesawi | Fatin Cedden

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Veterinary Sciences (Other)

Dergi:

Yuzuncu Yıl University Journal of Agricultural Sciences Vol.34 Issue 3, pp.505-514

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326934

pISSN:

1308-7576

Sheep

Embryo development

Ovarian tissue

Transport medium

Melatonin

Sayı

Makale detayları

Effect of Transport Condition on the Structural Integrity of Ovarian Tissue and the Development of Sheep Embryos In-Vitro

Yazarlar: Saıf Al-hafedh | Yahy Abood Kareem Alesawi | Fatin Cedden

Yayıncı: Van Yuzuncu Yıl University

Ana Başlıklar: Veterinary Sciences (Other)

Dergi: Yuzuncu Yıl University Journal of Agricultural Sciences Vol.34 Issue 3, pp.505-514

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001326934

pISSN: 1308-7576

Sheep

Embryo development

Ovarian tissue

Transport medium

Melatonin

Sayı

Yazarlar:

Saıf Al-hafedh | Yahy Abood Kareem Alesawi | Fatin Cedden

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Veterinary Sciences (Other)

Dergi:

Yuzuncu Yıl University Journal of Agricultural Sciences Vol.34 Issue 3, pp.505-514

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326934

pISSN:

1308-7576