Effects of L-2-ox&#1086;thiazolidine-4-carboxylic acid on the lung antioxidant defense system in an asthma mouse model

Lyudmil GEORGIEV TERZIEV; Veneta LYUBENOVA SHOPOVA; Violeta YORDANOVA DANCHEVA; Galya TZVETANOVA STAVREVA; Angelina MILCHEVA STOYANOVA

Makale detayları

Effects of L-2-oxоthiazolidine-4-carboxylic acid on the lung antioxidant defense system in an asthma mouse model

Yazarlar:

Lyudmil GEORGIEV TERZIEV | Veneta LYUBENOVA SHOPOVA | Violeta YORDANOVA DANCHEVA | Galya TZVETANOVA STAVREVA | Angelina MILCHEVA STOYANOVA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2012 Vol.42 Issue 5, pp.901-905

Belge Tipi:

Makale

Yayın Tarihi:

4/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000754048

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

We aim ed to study the effect of a glutathione precursor, L-2-oxothiazolidine-4-carboxylic acid (OTCA), on the lung antioxidant defense system in an animal asthma model.  Materials and methods: The study was carried out on 24 female C57BL/6 mice. The mice were divided into 4 treatment groups: group 1 - control group; group 2 - injected with ovalbumin (OVA) and given an OVA inhalant; group 3 - treated with OTCA and phosphate-buffered saline inhalant; and group 4 -injected with OVA and OTCA and given an OVA inhalant. Under sodium pentobarbital anesthesia the animals were killed by exsanguination 48 h after the last inhalation to obtain a lung homogenate. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GP) and the content of nonprotein sulfhydryl (NPSH) groups in lung homogenate were investigated.  Results: OVA decreased the activities of SOD (P = 0.007), CAT (P = 0.004), and GP (P = 0.05) and the NPSH content (P = 0.0008) in the lung homogenate compared with the control animals. Treatment with OVA and OTCA (group 4) resulted in a significant increase in the activities of CAT (P = 0.01) and GP (P = 0.05) and the NPSH content (P = 0.002) compared to the OVA group (group 2).  Conclusion: OTCA (160 mg/kg) restored the activities of basic enzymes in the lung antioxidant defense system in an OVA-induced asthma mouse model 48 h after the last nebulization.

 

We aim ed to study the effect of a glutathione precursor, L-2-oxothiazolidine-4-carboxylic acid (OTCA), on the lung antioxidant defense system in an animal asthma model.  Materials and methods: The study was carried out on 24 female C57BL/6 mice. The mice were divided into 4 treatment groups: group 1 - control group; group 2 - injected with ovalbumin (OVA) and given an OVA inhalant; group 3 - treated with OTCA and phosphate-buffered saline inhalant; and group 4 -injected with OVA and OTCA and given an OVA inhalant. Under sodium pentobarbital anesthesia the animals were killed by exsanguination 48 h after the last inhalation to obtain a lung homogenate. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GP) and the content of nonprotein sulfhydryl (NPSH) groups in lung homogenate were investigated.  Results: OVA decreased the activities of SOD (P = 0.007), CAT (P = 0.004), and GP (P = 0.05) and the NPSH content (P = 0.0008) in the lung homogenate compared with the control animals. Treatment with OVA and OTCA (group 4) resulted in a significant increase in the activities of CAT (P = 0.01) and GP (P = 0.05) and the NPSH content (P = 0.002) compared to the OVA group (group 2).  Conclusion: OTCA (160 mg/kg) restored the activities of basic enzymes in the lung antioxidant defense system in an OVA-induced asthma mouse model 48 h after the last nebulization.

Makale detayları

Effects of L-2-oxоthiazolidine-4-carboxylic acid on the lung antioxidant defense system in an asthma mouse model

Yazarlar:

Lyudmil GEORGIEV TERZIEV | Veneta LYUBENOVA SHOPOVA | Violeta YORDANOVA DANCHEVA | Galya TZVETANOVA STAVREVA | Angelina MILCHEVA STOYANOVA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2012 Vol.42 Issue 5, pp.901-905

Belge Tipi:

Makale

Yayın Tarihi:

4/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000754048

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Asthma

L-2-oхоthiazolidine-4-carboxylic acid

lung antioxidant defense system

Sayı

Makale detayları

Effects of L-2-ox&#1086;thiazolidine-4-carboxylic acid on the lung antioxidant defense system in an asthma mouse model

Yazarlar: Lyudmil GEORGIEV TERZIEV | Veneta LYUBENOVA SHOPOVA | Violeta YORDANOVA DANCHEVA | Galya TZVETANOVA STAVREVA | Angelina MILCHEVA STOYANOVA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2012 Vol.42 Issue 5, pp.901-905

Belge Tipi: Makale

Yayın Tarihi: 4/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000754048

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Asthma

L-2-o&#1093;&#1086;thiazolidine-4-carboxylic acid

lung antioxidant defense system

Sayı

Effects of L-2-oxоthiazolidine-4-carboxylic acid on the lung antioxidant defense system in an asthma mouse model

Yazarlar:

Lyudmil GEORGIEV TERZIEV | Veneta LYUBENOVA SHOPOVA | Violeta YORDANOVA DANCHEVA | Galya TZVETANOVA STAVREVA | Angelina MILCHEVA STOYANOVA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2012 Vol.42 Issue 5, pp.901-905

Belge Tipi:

Makale

Yayın Tarihi:

4/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000754048

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

L-2-oхоthiazolidine-4-carboxylic acid