Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation

Kevser EROL; Semra YİĞİTASLAN; Çiğdem ÜNEL; Bilgin KAYGISIZ; Engin YILDIRIM

Makale detayları

Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation

Yazarlar:

Kevser EROL | Semra YİĞİTASLAN | Çiğdem ÜNEL | Bilgin KAYGISIZ | Engin YILDIRIM

Yayıncı:

Trakya University

Dergi:

Balkan Medical Journal 2016 Vol.33 Issue 2, pp.0-0

Belge Tipi:

Makale

Yayın Tarihi:

2/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000759618

Sıklık:

İki Aylık

pISSN:

2146-3123

Özet:

 

Background: Calcium homeostasis is considered to be important in antineoplastic as well as in neurotoxic adverse effects of cisplatin.      Aims: This study aimed to investigate the role of Ca2+ in cisplatin neurotoxicity in cultured rat dorsal root ganglia (DRG) cells.      Study Design: Cell culture study.     Methods: DRG cells prepared from 1-day old Sprague-Dawley rats were used to determine the role of Ca2+ in the cisplatin (10-600 μM) neurotoxicity. The cells were incubated with cisplatin plus nimodipine (1-3 μM), dizocilpine (MK-801) (1-3 μM) or thapsigargin (100-300 nM). Toxicity of cisplatinon DRG cells was determined by the MTT assay.      Results: The neurotoxicity of cisplatin was significant when used in high concentrations (100-600 μM). Nimodipine (1 μM) but not MK-801 or thapsigargin prevented the neurotoxic effects of 200 μM of cisplatin.      Conclusion: Voltage-dependent calcium channels may play a role in cisplatin neurotoxicity.

Makale detayları

Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation

Yazarlar: Kevser EROL | Semra YİĞİTASLAN | Çiğdem ÜNEL | Bilgin KAYGISIZ | Engin YILDIRIM

Yayıncı: Trakya University

Dergi: Balkan Medical Journal 2016 Vol.33 Issue 2, pp.0-0

Belge Tipi: Makale

Yayın Tarihi: 2/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000759618

Sıklık: İki Aylık

pISSN: 2146-3123

Sayı

Yazarlar:

Kevser EROL | Semra YİĞİTASLAN | Çiğdem ÜNEL | Bilgin KAYGISIZ | Engin YILDIRIM

Yayıncı:

Trakya University

Dergi:

Balkan Medical Journal 2016 Vol.33 Issue 2, pp.0-0

Belge Tipi:

Makale

Yayın Tarihi:

2/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000759618

Sıklık:

İki Aylık

pISSN:

2146-3123