De novo Transcriptomic Analysis in Salvia officinalis L. Exposed to Nitric Oxide

Hakan Terzi; Mustafa Yıldız; Emre Pehlivan; Züleyha Kara; Mısra Taşdelen; Zeliha Atar

Makale detayları

De novo Transcriptomic Analysis in Salvia officinalis L. Exposed to Nitric Oxide

Yazarlar:

Hakan Terzi | Mustafa Yıldız | Emre Pehlivan | Züleyha Kara | Mısra Taşdelen | Zeliha Atar

Yayıncı:

Hacettepe University

Ana Başlıklar:

Systems Biology

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.53 Issue 4, pp.21-31

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359694

pISSN:

2687-475X

Anahtar kelimeler:

Özet:

 Common sage Salvia officinalis L. is a medicinal and aromatic plant that belongs to the Lamiaceae family. Nitric oxide NO is a key signaling molecules in cells whose level are increased in response to various stimuli. Therefore, in order to identify the genes involved in NO signaling, de novo RNA sequencing RNA-seq strategy was employed to evaluate the transcriptional profiles in sage leaves. In this study, sage plants were foliar sprayed with 100 µM NO donor diethylenetriamine NONOate. The leaf samples were then harvested after 24 hours of treatment for analyses. De novo assembly was performed on transcriptomic data using Trinity. Functional annotation and classification of the genes were performed by Gene Ontology GO and Kyoto Encyclopedia of Genes and Genomes KEGG analyses. As a result, 69 genes were differentially expressed in NO-treated leaves as compared to control. Among them, 9 genes were up-regulated, while 60 genes were down-regulated. GO analysis of the differentially expressed genes DEGs indicated significant enrichment in categories such as response to stress, defense response, and response to external stimuli between the control and NO-treated seedlings. RNA-seq analysis provided basic data on which molecular mechanisms may be involved depending on the DEGs in sage exposed to NO.

 Common sage Salvia officinalis L. is a medicinal and aromatic plant that belongs to the Lamiaceae family. Nitric oxide NO is a key signaling molecules in cells whose level are increased in response to various stimuli. Therefore, in order to identify the genes involved in NO signaling, de novo RNA sequencing RNA-seq strategy was employed to evaluate the transcriptional profiles in sage leaves. In this study, sage plants were foliar sprayed with 100 µM NO donor diethylenetriamine NONOate. The leaf samples were then harvested after 24 hours of treatment for analyses. De novo assembly was performed on transcriptomic data using Trinity. Functional annotation and classification of the genes were performed by Gene Ontology GO and Kyoto Encyclopedia of Genes and Genomes KEGG analyses. As a result, 69 genes were differentially expressed in NO-treated leaves as compared to control. Among them, 9 genes were up-regulated, while 60 genes were down-regulated. GO analysis of the differentially expressed genes DEGs indicated significant enrichment in categories such as response to stress, defense response, and response to external stimuli between the control and NO-treated seedlings. RNA-seq analysis provided basic data on which molecular mechanisms may be involved depending on the DEGs in sage exposed to NO.

 Common sage Salvia officinalis L. is a medicinal and aromatic plant that belongs to the Lamiaceae family. Nitric oxide NO is a key signaling molecules in cells whose level are increased in response to various stimuli. Therefore, in order to identify the genes involved in NO signaling, de novo RNA sequencing RNA-seq strategy was employed to evaluate the transcriptional profiles in sage leaves. In this study, sage plants were foliar sprayed with 100 µM NO donor diethylenetriamine NONOate. The leaf samples were then harvested after 24 hours of treatment for analyses. De novo assembly was performed on transcriptomic data using Trinity. Functional annotation and classification of the genes were performed by Gene Ontology GO and Kyoto Encyclopedia of Genes and Genomes KEGG analyses. As a result, 69 genes were differentially expressed in NO-treated leaves as compared to control. Among them, 9 genes were up-regulated, while 60 genes were down-regulated. GO analysis of the differentially expressed genes DEGs indicated significant enrichment in categories such as response to stress, defense response, and response to external stimuli between the control and NO-treated seedlings. RNA-seq analysis provided basic data on which molecular mechanisms may be involved depending on the DEGs in sage exposed to NO.

 Common sage Salvia officinalis L. is a medicinal and aromatic plant that belongs to the Lamiaceae family. Nitric oxide NO is a key signaling molecules in cells whose level are increased in response to various stimuli. Therefore, in order to identify the genes involved in NO signaling, de novo RNA sequencing RNA-seq strategy was employed to evaluate the transcriptional profiles in sage leaves. In this study, sage plants were foliar sprayed with 100 µM NO donor diethylenetriamine NONOate. The leaf samples were then harvested after 24 hours of treatment for analyses. De novo assembly was performed on transcriptomic data using Trinity. Functional annotation and classification of the genes were performed by Gene Ontology GO and Kyoto Encyclopedia of Genes and Genomes KEGG analyses. As a result, 69 genes were differentially expressed in NO-treated leaves as compared to control. Among them, 9 genes were up-regulated, while 60 genes were down-regulated. GO analysis of the differentially expressed genes DEGs indicated significant enrichment in categories such as response to stress, defense response, and response to external stimuli between the control and NO-treated seedlings. RNA-seq analysis provided basic data on which molecular mechanisms may be involved depending on the DEGs in sage exposed to NO.

Makale detayları

De novo Transcriptomic Analysis in Salvia officinalis L. Exposed to Nitric Oxide

Yazarlar:

Hakan Terzi | Mustafa Yıldız | Emre Pehlivan | Züleyha Kara | Mısra Taşdelen | Zeliha Atar

Yayıncı:

Hacettepe University

Ana Başlıklar:

Systems Biology

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.53 Issue 4, pp.21-31

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359694

pISSN:

2687-475X

Nitric oxide

RNA-seq

Salvia officinalis

transcriptomics

Sayı

Makale detayları

De novo Transcriptomic Analysis in Salvia officinalis L. Exposed to Nitric Oxide

Yazarlar: Hakan Terzi | Mustafa Yıldız | Emre Pehlivan | Züleyha Kara | Mısra Taşdelen | Zeliha Atar

Yayıncı: Hacettepe University

Ana Başlıklar: Systems Biology

Dergi: Hacettepe Journal of Biology and Chemistry Vol.53 Issue 4, pp.21-31

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001359694

pISSN: 2687-475X

Nitric oxide

RNA-seq

Salvia officinalis

transcriptomics

Sayı

Yazarlar:

Hakan Terzi | Mustafa Yıldız | Emre Pehlivan | Züleyha Kara | Mısra Taşdelen | Zeliha Atar

Yayıncı:

Hacettepe University

Ana Başlıklar:

Systems Biology

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.53 Issue 4, pp.21-31

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359694

pISSN:

2687-475X