Investigation of Glutathione S-Transferase-Alpha and Glutathione S-Transferase-Pi Expression Levels in Spermophilus xanthoprymnus and Meriones tristrami in Terms of Living Conditions and Natural Habitat Differences in Kırıkkale Province

Nahit Pamukoğlu; Serpil Oğuztüzün; Onur Dirican; Sezen Yılmaz Sarıaltın

Makale detayları

Investigation of Glutathione S-Transferase-Alpha and Glutathione S-Transferase-Pi Expression Levels in Spermophilus xanthoprymnus and Meriones tristrami in Terms of Living Conditions and Natural Habitat Differences in Kırıkkale Province

Yazarlar:

Nahit Pamukoğlu | Serpil Oğuztüzün | Onur Dirican | Sezen Yılmaz Sarıaltın

Yayıncı:

Bolu Abant Izzet Baysal University

Ana Başlıklar:

Hayvan Koruma | Hayvan Üretiminde Çevre Çalışmaları | Hayvan Yönetimi

Dergi:

Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi Vol.9 Issue 3, pp.448-459

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001305212

pISSN:

2149-8245

Anahtar kelimeler:

Özet:

 Glutathione S-transferase GST is a multifunctional enzyme that provides homeostasis by catalyzing the first step in the formation of the end product mercapturic acid in the detoxification metabolic pathway. Being found in mammals, insects, fish, birds, annelids, molluscs, and many microorganisms, GST takes part the elimination of toxic substances taken into body by consuming food, and their transport by binding non-substrate ligands e.g. heme and bilirubin with GSH. In addition, it can prevent reactive electrophilic compounds from harming the body by covalent bonding similar compounds to each other. These xenobiotic acceptors affected by GST include nitrogen halogen compounds, organophosphates, and polycyclic aromatic hydrocarbons. Xenobiotics are oxygenated by this enzyme system, the next mechanism of oxygenated products is more oxygenation, and these products become more easily soluble in water. In this study, Glutathione S-Transferase was detected in the liver tissue of Spermophilus xanthoprymnus and Meriones tristrami and its characteristic features were determined. For this purpose, the animals were anesthetized with sodium pentobarbital and their liver tissues were harvested. After necessary preparations were completed, the samples were analyzed by using immunohistochemical staining method and the expressions of GST isozymes were determined. As a result, glutathione s-transferase-alpha and glutathione s-transferase-pi expression levels were found to differ in Spermophilus xanthoprymnus and Meriones tristrami samples obtained from different localities of Kırıkkale province. Differences in GST enzyme expression in these species indicate that both species differ in their detoxification capacity and response to xenobiotics.

 Glutathione S-transferase GST is a multifunctional enzyme that provides homeostasis by catalyzing the first step in the formation of the end product mercapturic acid in the detoxification metabolic pathway. Being found in mammals, insects, fish, birds, annelids, molluscs, and many microorganisms, GST takes part the elimination of toxic substances taken into body by consuming food, and their transport by binding non-substrate ligands e.g. heme and bilirubin with GSH. In addition, it can prevent reactive electrophilic compounds from harming the body by covalent bonding similar compounds to each other. These xenobiotic acceptors affected by GST include nitrogen halogen compounds, organophosphates, and polycyclic aromatic hydrocarbons. Xenobiotics are oxygenated by this enzyme system, the next mechanism of oxygenated products is more oxygenation, and these products become more easily soluble in water. In this study, Glutathione S-Transferase was detected in the liver tissue of Spermophilus xanthoprymnus and Meriones tristrami and its characteristic features were determined. For this purpose, the animals were anesthetized with sodium pentobarbital and their liver tissues were harvested. After necessary preparations were completed, the samples were analyzed by using immunohistochemical staining method and the expressions of GST isozymes were determined. As a result, glutathione s-transferase-alpha and glutathione s-transferase-pi expression levels were found to differ in Spermophilus xanthoprymnus and Meriones tristrami samples obtained from different localities of Kırıkkale province. Differences in GST enzyme expression in these species indicate that both species differ in their detoxification capacity and response to xenobiotics.

 Glutathione S-transferase GST is a multifunctional enzyme that provides homeostasis by catalyzing the first step in the formation of the end product mercapturic acid in the detoxification metabolic pathway. Being found in mammals, insects, fish, birds, annelids, molluscs, and many microorganisms, GST takes part the elimination of toxic substances taken into body by consuming food, and their transport by binding non-substrate ligands e.g. heme and bilirubin with GSH. In addition, it can prevent reactive electrophilic compounds from harming the body by covalent bonding similar compounds to each other. These xenobiotic acceptors affected by GST include nitrogen halogen compounds, organophosphates, and polycyclic aromatic hydrocarbons. Xenobiotics are oxygenated by this enzyme system, the next mechanism of oxygenated products is more oxygenation, and these products become more easily soluble in water. In this study, Glutathione S-Transferase was detected in the liver tissue of Spermophilus xanthoprymnus and Meriones tristrami and its characteristic features were determined. For this purpose, the animals were anesthetized with sodium pentobarbital and their liver tissues were harvested. After necessary preparations were completed, the samples were analyzed by using immunohistochemical staining method and the expressions of GST isozymes were determined. As a result, glutathione s-transferase-alpha and glutathione s-transferase-pi expression levels were found to differ in Spermophilus xanthoprymnus and Meriones tristrami samples obtained from different localities of Kırıkkale province. Differences in GST enzyme expression in these species indicate that both species differ in their detoxification capacity and response to xenobiotics.

 Glutatyon S-transferaz GST çok fonksiyonlu bir enzim olup detoksifikasyon metabolik yolunda son ürün merkaptürik asit oluşumundaki ilk adımı katalize ederek homeostaz sağlar. Memeliler, böcekler, balıklar, kuşlar, annelidler, yumuşakçalar ve birçok mikroorganizmada bulunan GST, besinlerin tüketilmesiyle vücuda alınan toksik maddelerin vücuttan atılmasında ve substrat olmayan ligandlara örn. heme ve bilirubin bağlanarak taşınmasında görev alır. GSH. Ayrıca benzer bileşikleri birbirine kovalent yolla bağlayarak reaktif elektrofilik bileşiklerin vücuda zarar vermesini engelleyebilmektedir. GSTden etkilenen bu ksenobiyotik alıcılar, nitrojen halojen bileşikleri, organofosfatlar ve polisiklik aromatik hidrokarbonları içerir. Ksenobiyotikler bu enzim sistemi tarafından oksijenlenir, oksijenli ürünlerin bir sonraki mekanizması daha fazla oksijenlenme olur ve bu ürünler suda daha kolay çözünür hale gelir. Bu çalışmada Spermophilus xanthoprymnus ve Meriones tristraminin karaciğer dokusunda Glutatyon S-Transferaz saptanmış ve karakteristik özellikleri belirlenmiştir. Bu amaçla hayvanlara sodyum pentobarbital ile anestezi uygulandı ve karaciğer dokuları alındı. Gerekli hazırlıklar tamamlandıktan sonra örnekler immunohistokimyasal boyama yöntemi kullanılarak analiz edildi ve GST izozimlerinin ifadeleri belirlendi. Sonuç olarak, Kırıkkale ilinin farklı lokalitelerinden temin edilen Spermophilus xanthoprymnus ve Meriones tristrami örneklerinde glutatyon s-transferaz alfa ve glutatyon s-transferaz-pi ifade düzeylerinin farklı olduğu bulundu. Bu türlerdeki GST enzim ekspresyon farklılıkları her iki türün detoksifikasyon kapasitesinin ve ksenobiyotiklere vereceği cevabın farklı olduğunu gösterir.

Makale detayları

Investigation of Glutathione S-Transferase-Alpha and Glutathione S-Transferase-Pi Expression Levels in Spermophilus xanthoprymnus and Meriones tristrami in Terms of Living Conditions and Natural Habitat Differences in Kırıkkale Province

Yazarlar:

Nahit Pamukoğlu | Serpil Oğuztüzün | Onur Dirican | Sezen Yılmaz Sarıaltın

Yayıncı:

Bolu Abant Izzet Baysal University

Ana Başlıklar:

Hayvan Koruma | Hayvan Üretiminde Çevre Çalışmaları | Hayvan Yönetimi

Dergi:

Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi Vol.9 Issue 3, pp.448-459

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001305212

pISSN:

2149-8245

Glutathione S-transferase

habitat differences

immunohistochemistry

Meriones tristrami

Spermophilus xanthoprymnusGlutathione S-transferase

habitat farklılıkları

immünohistokimya

Meriones tristrami

Spermophilus xanthoprymnus

Sayı

Makale detayları

Yazarlar: Nahit Pamukoğlu | Serpil Oğuztüzün | Onur Dirican | Sezen Yılmaz Sarıaltın

Yayıncı: Bolu Abant Izzet Baysal University

Ana Başlıklar: Hayvan Koruma | Hayvan Üretiminde Çevre Çalışmaları | Hayvan Yönetimi

Dergi: Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi Vol.9 Issue 3, pp.448-459

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001305212

pISSN: 2149-8245

Sayı

Yazarlar:

Nahit Pamukoğlu | Serpil Oğuztüzün | Onur Dirican | Sezen Yılmaz Sarıaltın

Yayıncı:

Bolu Abant Izzet Baysal University

Ana Başlıklar:

Hayvan Koruma | Hayvan Üretiminde Çevre Çalışmaları | Hayvan Yönetimi

Dergi:

Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi Vol.9 Issue 3, pp.448-459

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001305212

pISSN:

2149-8245