Saponin rich Gypsophila eriocalyx Boiss methanolic extract (GEME) exhibit anticancerogenic effect against neuroblastoma cancer cell line (SH-SY5Y)

Serap Niğdelioğlu Dolanbay

Makale detayları

Saponin rich Gypsophila eriocalyx Boiss methanolic extract (GEME) exhibit anticancerogenic effect against neuroblastoma cancer cell line (SH-SY5Y)

Yazarlar:

Serap Niğdelioğlu Dolanbay

Yayıncı:

Cukurova University

Ana Başlıklar:

Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç) | Moleküler Hedefler

Dergi:

Cukurova Medical Journal Vol.49 Issue 1, pp.31-39

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314675

pISSN:

2602-3032

Anahtar kelimeler:

Özet:

 Purpose: Herein, we sought to determine whether of saponin rich Gypsophila eriocalyx methanol extract GEME would exhibit any anti-carcinogenic activity on neuroblastoma cancer cell line SH-SY5Y. Materials and Methods: We therefore determined GEMEs saponin composition using LC-MS analysis, its impact on cell viability using MTT analysis, flow cytometry, and to find out its impact on apoptosis using qRT-PCR analysis. Results: In the LC-MS analysis we determined that GEME contained high amounts of saponin 0.05-0.48 µgg. We determined that GEME had an IC50 dose of 100 μgmL at 48 hours. GEME had the effect of substantially increasing the percentage of apoptotic cells 5.19 and 65.21 and disruption of mitochondria 46.18. We also demonstrated that BCL2 gene expression 2.76 fold was significantly reduced than that of the control while BAX 2.21 fold, CASP3 2.43 fold, CASP7 2.98 fold, CASP8 2.23 fold, CASP9 2.78 fold, and CYCS 2.12 fold genes were expressed significantly higher than those of the control. Conclusion: Considering the findings, it becomes clear that saponin-rich GEME stands out as a significant anticarcinogenic agent. Its remarkable efficacy is demonstrated through its capabilities to notably reduce cell viability, effectively trigger apoptosis, and significantly increase the rate of mitochondrial disruption in cancer cells.

 Purpose: Herein, we sought to determine whether of saponin rich Gypsophila eriocalyx methanol extract GEME would exhibit any anti-carcinogenic activity on neuroblastoma cancer cell line SH-SY5Y. Materials and Methods: We therefore determined GEMEs saponin composition using LC-MS analysis, its impact on cell viability using MTT analysis, flow cytometry, and to find out its impact on apoptosis using qRT-PCR analysis. Results: In the LC-MS analysis we determined that GEME contained high amounts of saponin 0.05-0.48 µgg. We determined that GEME had an IC50 dose of 100 μgmL at 48 hours. GEME had the effect of substantially increasing the percentage of apoptotic cells 5.19 and 65.21 and disruption of mitochondria 46.18. We also demonstrated that BCL2 gene expression 2.76 fold was significantly reduced than that of the control while BAX 2.21 fold, CASP3 2.43 fold, CASP7 2.98 fold, CASP8 2.23 fold, CASP9 2.78 fold, and CYCS 2.12 fold genes were expressed significantly higher than those of the control. Conclusion: Considering the findings, it becomes clear that saponin-rich GEME stands out as a significant anticarcinogenic agent. Its remarkable efficacy is demonstrated through its capabilities to notably reduce cell viability, effectively trigger apoptosis, and significantly increase the rate of mitochondrial disruption in cancer cells.

 Purpose: Herein, we sought to determine whether of saponin rich Gypsophila eriocalyx methanol extract GEME would exhibit any anti-carcinogenic activity on neuroblastoma cancer cell line SH-SY5Y. Materials and Methods: We therefore determined GEMEs saponin composition using LC-MS analysis, its impact on cell viability using MTT analysis, flow cytometry, and to find out its impact on apoptosis using qRT-PCR analysis. Results: In the LC-MS analysis we determined that GEME contained high amounts of saponin 0.05-0.48 µgg. We determined that GEME had an IC50 dose of 100 μgmL at 48 hours. GEME had the effect of substantially increasing the percentage of apoptotic cells 5.19 and 65.21 and disruption of mitochondria 46.18. We also demonstrated that BCL2 gene expression 2.76 fold was significantly reduced than that of the control while BAX 2.21 fold, CASP3 2.43 fold, CASP7 2.98 fold, CASP8 2.23 fold, CASP9 2.78 fold, and CYCS 2.12 fold genes were expressed significantly higher than those of the control. Conclusion: Considering the findings, it becomes clear that saponin-rich GEME stands out as a significant anticarcinogenic agent. Its remarkable efficacy is demonstrated through its capabilities to notably reduce cell viability, effectively trigger apoptosis, and significantly increase the rate of mitochondrial disruption in cancer cells.

 Amaç: Bu çalışmada saponinden zengini Gypsophila eriocalyx metanol ekstraktı GEMEnın nöroblastom kanserr hücre hattı SH-SY5Y üzerinde herhangi bir anti-karsinojenik aktivite gösterip göstermediğini belirlemeyi amaçladık. Gereç ve Yöntem: Bu nedenle GEMEnin saponin kompozisyonunu LC-MS analizi kullanarak, hücre canlılığı üzerindeki etkisini MTT analizi, apoptozis üzerindeki etkisini akış sitometrisi ve qRT-PCR analizi ile belirledik. Bulgular: LC-MS analizinde, GEMEnin yüksek miktarda saponin 0,05-0,48 µgg içerdiğini belirledik. GEMEnin 48 saatte 100 μgmL IC50 dozuna sahip olduğunu belirledik. GEMEnin apoptotik hücrelerin yüzdesini 5,19 ve 65,21 önemli ölçüde arttırma ve mitokondrilerin bozulmasına 46,18 etkisi oldu. Ayrıca, BCL2 2,76 kat gen ekspresyonunun kontrol grubundan önemli ölçüde düşük olduğunu; BAX 2,21 kat, CASP3 2,43 kat, CASP7 2,98 kat, CASP8 2,23 kat, CASP9 2,78 kat ve CYCS 2,12 kat genlerinin ise kontrol grubundan anlamlı derecede yüksek olduğunu gösterdik. Sonuç: Bulgulara bakıldığında saponin açısından zengin GEMEnin önemli bir antikarsinojenik ajan olarak öne çıktığı ortaya çıkmaktadır. Dikkate değer etkinliği, hücre canlılığını önemli ölçüde azaltma, apoptozu etkili bir şekilde tetikleme ve kanser hücrelerinde mitokondriyal bozulma oranını önemli ölçüde artırma yetenekleriyle kanıtlanmıştır.

Makale detayları

Saponin rich Gypsophila eriocalyx Boiss methanolic extract (GEME) exhibit anticancerogenic effect against neuroblastoma cancer cell line (SH-SY5Y)

Yazarlar:

Serap Niğdelioğlu Dolanbay

Yayıncı:

Cukurova University

Ana Başlıklar:

Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç) | Moleküler Hedefler

Dergi:

Cukurova Medical Journal Vol.49 Issue 1, pp.31-39

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314675

pISSN:

2602-3032

Anticancerogenic effect

Gypsophila eriocalyx Boiss

methanolic extract

neuroblastoma cancer cell line (SH-SY5Y)Antikarsinojenik etki

Gypsophila eriocalyx Boiss

metanol ekstraktı

nöroblastom kanser hücre hattı (SH-SY5Y)

Sayı

Makale detayları

Yazarlar: Serap Niğdelioğlu Dolanbay

Yayıncı: Cukurova University

Ana Başlıklar: Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç) | Moleküler Hedefler

Dergi: Cukurova Medical Journal Vol.49 Issue 1, pp.31-39

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001314675

pISSN: 2602-3032

Sayı

Yazarlar:

Serap Niğdelioğlu Dolanbay

Yayıncı:

Cukurova University

Ana Başlıklar:

Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç) | Moleküler Hedefler

Dergi:

Cukurova Medical Journal Vol.49 Issue 1, pp.31-39

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314675

pISSN:

2602-3032