Rapid identification of Klebsiella pneumoniae isolates from various samples with biosensor and genotyping

Sümeyra SAVAŞ

doi:https://doi.org/10.33988/auvfd.568339

Makale detayları

Rapid identification of Klebsiella pneumoniae isolates from various samples with biosensor and genotyping

Yazarlar:

Sümeyra SAVAŞ

Ana Başlıklar:

Hayvancılık

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2019 Vol.66 Issue 4, pp.397-405

Belge Tipi:

Makale

Yayın Tarihi:

9/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000849594

DOI:

https://doi.org/10.33988/auvfd.568339

Özet:

 


The most
important cause of Klebsiella spp.
contamination of drinking water is the leakage of animal faeces into drinking
water sources. Recently, the biosensor technology has quickly begun to replace
other methods with its faster finding and reliability. The aim of this study
was to investigate the reliability of the biosensor technology in the rapid
detection of Klebsiella pneumoniae (K. pneumoniae) and to determine the
presence of the relationship between K.
pneumoniae isolates isolated from the drinking water thought to be
contaminated by animal faeces and the clinical isolates. For this purpose,
portable, microfluidic electrochemical sensor device version 2 (V2) was used
for the detection of K. pneumoniae
and results were confirmed with VITEK MALDI-TOF Mass Spectrometry (VITEK MS)
automated system. For the molecular typing of K. pneumoniae isolates, pulsed-field gel electrophoresis (PFGE) and
multiple locus variable-number tandem repeat analysis (MLVA) methods were
employed and the results were compared. For these bacteria, the most appropriate
typing method was tried to be determined comparatively. PFGE analysis indicated
the presence of six different strains, while MLVA divided them into 23
clusters. Clonal relationships were viewed between environmental and clinical
isolates. The main goal of this paper is to present, the detailed report of the
comparison of the samples isolated from drinking water, animal and human faeces
for K. pneumoniae. To accomplish of
this goal we introduced that MLVA and PFGE methods. Also, gold nanoparticies
enhanced electrochemical biosensor device is used for the determination of K. pneumoniae for the first time.



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Makale detayları

Rapid identification of Klebsiella pneumoniae isolates from various samples with biosensor and genotyping

Yazarlar: Sümeyra SAVAŞ

Ana Başlıklar: Hayvancılık

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi 2019 Vol.66 Issue 4, pp.397-405

Belge Tipi: Makale

Yayın Tarihi: 9/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000849594

DOI: https://doi.org/10.33988/auvfd.568339

Sayı

Yazarlar:

Sümeyra SAVAŞ

Ana Başlıklar:

Hayvancılık

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2019 Vol.66 Issue 4, pp.397-405

Belge Tipi:

Makale

Yayın Tarihi:

9/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000849594

DOI:

https://doi.org/10.33988/auvfd.568339