Eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes

Ayse Tarbin Jannuzzı

Makale detayları

Eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes

Yazarlar:

Ayse Tarbin Jannuzzı

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Toxicology

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 2, pp.354-361

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346343

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Eugenol is a phenolic compound isolated from clove essential oil. It is used in dentistry, fragrance, cosmetic, and food industries. According to the fragrance ingredient safety assessment report, eugenol does not cause phototoxic reactions and genotoxicity. However, its effect on UV-induced cytotoxicity and genotoxicity has not been well examined. Here in this study, cytotoxic and genotoxic effects of eugenol are investigated on UVA-induced damage using human keratinocyte cells HaCaT. HaCaT cells were treated with increasing concentrations of eugenol 10-500 µM for 1 hour and irradiated with 5-10-15 Jcm2 UVA. 24 hours later the neutral red uptake NRU assay was used to evaluate cytotoxicity. For genotoxicity assay cells were exposed to 1-10 µM eugenol for one hour and non-cytotoxic UVA irradiation doses 1, 2.5 Jcm2 were used. The alkaline comet assay was carried out immediately after the UVA irradiation to measure the genotoxic potential of eugenol. The cytotoxicity assay results indicate that eugenol caused a cytotoxic effect in a dose-dependent manner in HaCaT cells and increasing doses of UVA-irradiation enhanced the cytotoxic effect of eugenol. The alkaline comet assay results showed that eugenol causes DNA single-strand breaks and increasing doses of UVA-irradiation aggravates the genotoxic potential of eugenol. These data demonstrate that eugenol has cytotoxic and genotoxic potential and eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes.

 Eugenol is a phenolic compound isolated from clove essential oil. It is used in dentistry, fragrance, cosmetic, and food industries. According to the fragrance ingredient safety assessment report, eugenol does not cause phototoxic reactions and genotoxicity. However, its effect on UV-induced cytotoxicity and genotoxicity has not been well examined. Here in this study, cytotoxic and genotoxic effects of eugenol are investigated on UVA-induced damage using human keratinocyte cells HaCaT. HaCaT cells were treated with increasing concentrations of eugenol 10-500 µM for 1 hour and irradiated with 5-10-15 Jcm2 UVA. 24 hours later the neutral red uptake NRU assay was used to evaluate cytotoxicity. For genotoxicity assay cells were exposed to 1-10 µM eugenol for one hour and non-cytotoxic UVA irradiation doses 1, 2.5 Jcm2 were used. The alkaline comet assay was carried out immediately after the UVA irradiation to measure the genotoxic potential of eugenol. The cytotoxicity assay results indicate that eugenol caused a cytotoxic effect in a dose-dependent manner in HaCaT cells and increasing doses of UVA-irradiation enhanced the cytotoxic effect of eugenol. The alkaline comet assay results showed that eugenol causes DNA single-strand breaks and increasing doses of UVA-irradiation aggravates the genotoxic potential of eugenol. These data demonstrate that eugenol has cytotoxic and genotoxic potential and eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes.

 Eugenol is a phenolic compound isolated from clove essential oil. It is used in dentistry, fragrance, cosmetic, and food industries. According to the fragrance ingredient safety assessment report, eugenol does not cause phototoxic reactions and genotoxicity. However, its effect on UV-induced cytotoxicity and genotoxicity has not been well examined. Here in this study, cytotoxic and genotoxic effects of eugenol are investigated on UVA-induced damage using human keratinocyte cells HaCaT. HaCaT cells were treated with increasing concentrations of eugenol 10-500 µM for 1 hour and irradiated with 5-10-15 Jcm2 UVA. 24 hours later the neutral red uptake NRU assay was used to evaluate cytotoxicity. For genotoxicity assay cells were exposed to 1-10 µM eugenol for one hour and non-cytotoxic UVA irradiation doses 1, 2.5 Jcm2 were used. The alkaline comet assay was carried out immediately after the UVA irradiation to measure the genotoxic potential of eugenol. The cytotoxicity assay results indicate that eugenol caused a cytotoxic effect in a dose-dependent manner in HaCaT cells and increasing doses of UVA-irradiation enhanced the cytotoxic effect of eugenol. The alkaline comet assay results showed that eugenol causes DNA single-strand breaks and increasing doses of UVA-irradiation aggravates the genotoxic potential of eugenol. These data demonstrate that eugenol has cytotoxic and genotoxic potential and eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes.

 Eugenol is a phenolic compound isolated from clove essential oil. It is used in dentistry, fragrance, cosmetic, and food industries. According to the fragrance ingredient safety assessment report, eugenol does not cause phototoxic reactions and genotoxicity. However, its effect on UV-induced cytotoxicity and genotoxicity has not been well examined. Here in this study, cytotoxic and genotoxic effects of eugenol are investigated on UVA-induced damage using human keratinocyte cells HaCaT. HaCaT cells were treated with increasing concentrations of eugenol 10-500 µM for 1 hour and irradiated with 5-10-15 Jcm2 UVA. 24 hours later the neutral red uptake NRU assay was used to evaluate cytotoxicity. For genotoxicity assay cells were exposed to 1-10 µM eugenol for one hour and non-cytotoxic UVA irradiation doses 1, 2.5 Jcm2 were used. The alkaline comet assay was carried out immediately after the UVA irradiation to measure the genotoxic potential of eugenol. The cytotoxicity assay results indicate that eugenol caused a cytotoxic effect in a dose-dependent manner in HaCaT cells and increasing doses of UVA-irradiation enhanced the cytotoxic effect of eugenol. The alkaline comet assay results showed that eugenol causes DNA single-strand breaks and increasing doses of UVA-irradiation aggravates the genotoxic potential of eugenol. These data demonstrate that eugenol has cytotoxic and genotoxic potential and eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes.

Makale detayları

Eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes

Yazarlar:

Ayse Tarbin Jannuzzı

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Toxicology

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 2, pp.354-361

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346343

pISSN:

2630-6344

Eugenol

UVA

DNA damage

human keratinocyte

cytotoxicity

genotoxicity

Sayı

Makale detayları

Eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes

Yazarlar: Ayse Tarbin Jannuzzı

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Toxicology

Dergi: Journal of Research in Pharmacy Vol.26 Issue 2, pp.354-361

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346343

pISSN: 2630-6344

Eugenol

UVA

DNA damage

human keratinocyte

cytotoxicity

genotoxicity

Sayı

Yazarlar:

Ayse Tarbin Jannuzzı

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Toxicology

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 2, pp.354-361

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346343

pISSN:

2630-6344