The role of outer inflammatory protein A (OipA) in vaccination of the  C57BL/6 mouse model infected by Helicobacter pylori

Mohaddese MAHBOUBI; Tahereh FALSAFI; Majid SADEGHIZADEH; Fatemeh MAHJOUB

Makale detayları

The role of outer inflammatory protein A (OipA) in vaccination of the C57BL/6 mouse model infected by Helicobacter pylori

Yazarlar:

Mohaddese MAHBOUBI | Tahereh FALSAFI | Majid SADEGHIZADEH | Fatemeh MAHJOUB

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2017 Vol.47 Issue 1, pp.326-333

Belge Tipi:

Makale

Yayın Tarihi:

1/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000751628

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Özet:

 

Background/aim: Outer inflammatory protein A (OipA) is an important adhesin of Helicobacter pylori. Our goal was to assess the role of OipA in protection of C57BL/6 mice against H. pylori.   Materials and methods: C57BL/6 mice were mucosally immunized with recombinant OipA protein, OipA + propolis, propolis, and phosphate-buffered saline. After vaccination, anti-OipA IgA was measured. Mice were challenged three times with 5 x 107 CFU of the H. pylori B19 strain. Two weeks later, bacterial colonization and inflammation in the stomach was analyzed using standard methods.   Results: The CFU number in the OipA group was significantly (P < 0.05) lower than that of the control. The CFU number in the OipA + propolis group was higher than those of the OipA and propolis groups. IgA titers were significantly higher (P < 0.0001) in the OipA group compared to the control and OipA + propolis groups. Propolis did not play an adjuvant effect but it interfered with the efficient vaccine effect of OipA.  Conclusion: Results show the effect of vaccination by OipA in protection of the mouse model and the importance of OipA in H. pylori pathogenesis. OipA may be proposed as a suitable oral vaccine candidate against H. pylori infection; however, further study is required to determine adjuvant or adverse effects of propolis toward OipA.

Makale detayları

The role of outer inflammatory protein A (OipA) in vaccination of the C57BL/6 mouse model infected by Helicobacter pylori

Yazarlar: Mohaddese MAHBOUBI | Tahereh FALSAFI | Majid SADEGHIZADEH | Fatemeh MAHJOUB

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2017 Vol.47 Issue 1, pp.326-333

Belge Tipi: Makale

Yayın Tarihi: 1/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000751628

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Sayı

Yazarlar:

Mohaddese MAHBOUBI | Tahereh FALSAFI | Majid SADEGHIZADEH | Fatemeh MAHJOUB

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2017 Vol.47 Issue 1, pp.326-333

Belge Tipi:

Makale

Yayın Tarihi:

1/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000751628

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144