Nucleic Acid Extraction from Clinical Specimens for PCR Applications

Ashabil AYGAN

Makale detayları

Nucleic Acid Extraction from Clinical Specimens for PCR Applications

Yazarlar:

Ashabil AYGAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2006 Vol.30 Issue 2, pp.107-120

Belge Tipi:

Makale

Yayın Tarihi:

1/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000813401

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Polymerase chain reaction (PCR) has been widely used due to its high specificity, sensitivity, and rapid turn-around time. However, the major limitation of PCR based diagnosis is inhibition of the reaction caused by a variety of components within clinical specimens. The demand for PCR diagnosis in medical microbiology has highlighted the need for efficient methods of nucleic acid extraction. Although there has been progress in the simplification and purification of microbial nucleic acids, many researchers' procedures are still inconvenient for routine use with any specimen and a universal method has not been devised yet. In this review, some of the basic principles for extraction procedures that have been used to date and the present situation of nucleic acid extraction are summarized.

 

Polymerase chain reaction (PCR) has been widely used due to its high specificity, sensitivity, and rapid turn-around time. However, the major limitation of PCR based diagnosis is inhibition of the reaction caused by a variety of components within clinical specimens. The demand for PCR diagnosis in medical microbiology has highlighted the need for efficient methods of nucleic acid extraction. Although there has been progress in the simplification and purification of microbial nucleic acids, many researchers' procedures are still inconvenient for routine use with any specimen and a universal method has not been devised yet. In this review, some of the basic principles for extraction procedures that have been used to date and the present situation of nucleic acid extraction are summarized.

Makale detayları

Nucleic Acid Extraction from Clinical Specimens for PCR Applications

Yazarlar:

Ashabil AYGAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2006 Vol.30 Issue 2, pp.107-120

Belge Tipi:

Makale

Yayın Tarihi:

1/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000813401

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

PCR

Clinical Specimens

Inhibitors

DNA extraction

Sayı

Makale detayları

Nucleic Acid Extraction from Clinical Specimens for PCR Applications

Yazarlar: Ashabil AYGAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2006 Vol.30 Issue 2, pp.107-120

Belge Tipi: Makale

Yayın Tarihi: 1/2006

Belge Dili: Türkçe

Document ID (Org No.): 2000813401

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

PCR

Clinical Specimens

Inhibitors

DNA extraction

Sayı

Yazarlar:

Ashabil AYGAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2006 Vol.30 Issue 2, pp.107-120

Belge Tipi:

Makale

Yayın Tarihi:

1/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000813401

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152